High-throughput micro-plate HCl-vanillin assay for screening tannin content in sorghum grain

Journal of the Science of Food and Agriculture

Volumn 94, Issue 10, 2014, Pages 2133-2136

  Herald, Thomas J ^a   Gadgil, Priyadarshini ^a   Perumal, Ramasamy ^b   Bean, Scott R ^a   Wilson, Jeff D ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b KANSAS STATE UNIVERSITY   (United States)

Author keywords

96 well plate; Sorghum; Tannin; Vanillin

Indexed keywords

BENZALDEHYDE DERIVATIVE; TANNIN DERIVATIVE; VANILLIN;

CEREAL; CHEMISTRY; HIGH THROUGHPUT SCREENING; HUMAN; PLANT SEED; PROCEDURES; REPRODUCIBILITY; SORGHUM; STANDARDS; VALIDATION STUDY;

BENZALDEHYDES; CEREALS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; REPRODUCIBILITY OF RESULTS; SEEDS; SORGHUM; TANNINS;

EID: 84901913892     PISSN: 00225142     EISSN: 10970010     Source Type: Journal    
DOI: 10.1002/jsfa.6538     Document Type: Article

References (11)

1. Austin DL, Turner, ND, Mcdonough CM and Rooney LW, Effects of brans from specialty sorghum varieties on in vitro starch digestibility of soft and hard endosperm porridges. Cereal Chem 89:190-197 (2012).
2. Burdette A, Garner PL, Mayer EP, Hargorve JL, Hartle DK and Greenspan P, Anti-inflammatory activity of select sorghum (Sorghum bicolor) brans.J Med Food 13:879-887 (2010).
3. Awika JM and Rooney LW, Sorghum phytochemicals and their potential impact on human health. Phytochemistry 65:1199-1221 (2004).
4. Hagerman AE, Riedl KM, Jones GA, Sovik K., Ritchard NT, Hartzfeld PW et al., High molecular weight plant polyphenolics (tannins) as biological antioxidants. J Agric Food Chem 46:1887-189 (1998).
5. Waniska R, Hugo LF and Rooney LW, Practical methods to determine the presence of tannins in sorghum. J Appl Poult Res 1:122-128 (1992).
6. Burns RE, Method of estimation of tannin in grain sorghum. J Agron 63:511-512 (1971).
7. Earp CF, Akingbala JO, Ring SH and Rooney LW, Evaluation of several methods to determine tannins in sorghums with varying kernel characteristics. Cereal Chem 58:224-238 (1981).
8. Sarkar SK and Howarth RE, Specificity of the vanillin test for flavanols. J Agric Food Chem 24:317-320 (1976).
9. Price ML, Van Scoyoc S and Butler LG, A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain. J Agric Food Chem 26:1214-1218 (1978).
10. Cheng Z, Moore J and Yu L, High-throughput relative DPPH radical scavenging capacity assay J Agric Food Chem 54:7429-7436 (2006).
11. Herald TJ, Gadgil P and Tilley M, High-throughput micro plate assays for screening flavonoid content and DPPH-scavenging activity in sorghum bran and flour. J Sci Food Agric 92:2326-2331 (2012).