Rapid detection of new delhi metallo-β-lactamase gene and variants coding for carbapenemases with different activities by use of a PCR-based in vitro protein expression method

Journal of Clinical Microbiology

Volumn 52, Issue 6, 2014, Pages 1947-1953

  Huang, Li ^a,c   Hu, Xiumei ^b,d   Zhou, Man ^a   Yang, Yinmei ^b   Qiao, Jinjuan ^a,c   Wang, Dianbing ^a   Yu, Junping ^a   Cui, Zongqiang ^a   Zhang, Zhiping ^a   Zhang, Xian En ^a   Wei, Hongping ^a  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

^b GUANGZHOU FIRST PEOPLE'S HOSPITAL   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^d SOUTHERN MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CARBAPENEMASE; METALLO BETA LACTAMASE; BETA LACTAMASE; BETA-LACTAMASE NDM-1;

AMPLICON; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATE; CARBAPENEMASE PRODUCING ENTEROBACTERIACEAE; CELL FREE SYSTEM; CONTROLLED STUDY; ENZYME ASSAY; GENE ACTIVITY; GENE DOSAGE; GENETIC VARIABILITY; IN VITRO STUDY; LIMIT OF DETECTION; LONG FRAGMENT REAL TIME POLYMERASE CHAIN REACTION; MOLECULAR CLONING; NEW DELHI METALLO BETA LACTAMASE 1 GENE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REACTION TIME; REAL TIME POLYMERASE CHAIN REACTION; SCREENING TEST; BIOSYNTHESIS; ENZYMOLOGY; EVALUATION STUDY; GENE EXPRESSION PROFILING; GENETICS; GRAM NEGATIVE BACTERIUM; HUMAN; ISOLATION AND PURIFICATION; PROCEDURES; SENSITIVITY AND SPECIFICITY; TIME;

BETA-LACTAMASES; GENE EXPRESSION PROFILING; GRAM-NEGATIVE BACTERIA; HUMANS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 84901649090     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.03363-13     Document Type: Article

References (33)

1. Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, Walsh TR. 2009. Characterization of a new metallo-lactamase gene, blaNDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob. Agents Chemother. 53:5046-5054. http://dx.doi.org/10.1128/AAC.00774-09.
2. Walsh TR, Weeks J, Livermore DM, Toleman MA. 2011. Dissemination of NDM-1 positive bacteria in the New Delhi environment and its implications for human health: an environmental point prevalence study. Lancet Infect. Dis. 11: 355-362. http://dx.doi.org/10.1016/S1473-3099(11)70059-7.
3. Moellering RC, Jr. 2010. NDM-1-a cause for worldwide concern. N. Engl. J. Med. 363:2377-2379. http://dx.doi.org/10.1056/NEJMp1011715.
4. Kumarasamy KK, Toleman MA, Walsh TR, Bagaria J, Butt F, Balakrishnan R, Chaudhary U, Doumith M, Giske CG, Irfan S, Krishnan P, Kumar AV, Maharjan S, Mushtaq S, Noorie T, Paterson DL, Pearson A, Perry C, Pike R, Rao B, Ray U, Sarma JB, Sharma M, Sheridan E, Thirunarayan MA, Turton J, Upadhyay S, Warner M, Welfare W, Livermore DM, Woodford N. 2010. Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect. Dis. 10:597-602. http://dx.doi.org/10. 1016/S1473-3099(10)70143-2.
5. Chen Y, Zhou Z, Jiang Y, Yu Y. 2011. Emergence of NDM-1-producing Acinetobacter baumannii in China. J. Antimicrob. Chemother. 66:1255-1259. http://dx.doi.org/10.1093/jac/dkr082.
6. Shibl AM, Memish ZA, Al-Agamy MH, Senok AC, Begum S, Assiri A. 2013. Emergence of OXA-48 and NDM-1 positive Klebsiella pneumoniae in Riyadh, Saudi Arabia. Int. J. Antimicrob. Agents 42:S83-S84. http://dx.doi.org/10.1016/S0924- 8579(13)70375-3.
7. Kaase M, Nordmann P, Wichelhaus TA, Gatermann SG, Bonnin RA, Poirel L. 2011. NDM-2 carbapenemase in Acinetobacter baumannii from Egypt. J. Antimicrob. Chemother. 66:1260-1262. http://dx.doi.org/10.1093/jac/dkr135.
8. Rogers BA, Sidjabat HE, Silvey A, Anderson TL, Perera S, Li J, Paterson DL. 2013. Treatment options for New Delhi metallo-beta-lactamaseharboring enterobacteriaceae. Microb. Drug Resist. 19:100-103. http://dx.doi.org/10.1089/ mdr.2012.0063.
9. Nordmann P, Boulanger AE, Poirel L. 2012. NDM-4 metallo-lactamase with increased carbapenemase activity from Escherichia coli. Antimicrob. Agents Chemother. 56:2184-2186. http://dx.doi.org/10.1128/AAC.05961-11.
10. Hornsey M, Phee L, Wareham DW. 2011. A novel variant, NDM-5, of the New Delhi metallo-beta-lactamase in a multidrug-resistant Escherichia coli ST648 isolate recovered from a patient in the United Kingdom. Antimicrob. Agents Chemother. 55:5952-5954. http://dx.doi.org/10.1128/AAC.05108-11.
11. Williamson DA, Sidjabat HE, Freeman JT, Roberts SA, Silvey A, Woodhouse R, Mowat E, Dyet K, Paterson DL, Blackmore T, Burns A, Heffernan H. 2012. Identification and molecular characterisation of New Delhi metallo-beta- lactamase-1 (NDM-1)-and NDM-6-producing Enterobacteriaceae from New Zealand hospitals. Int. J. Antimicrob. Agents 39:529-533. http://dx.doi.org/10.1016/j. ijantimicag.2012.02.017.
12. Gottig S, Hamprecht AG, Christ S, Kempf VA, Wichelhaus TA. 2013. Detection of NDM-7 in Germany, a new variant of the New Delhi metallobeta-lactamase with increased carbapenemase activity. J. Antimicrob. Chemother. 68:1737-1740. http://dx.doi.org/10.1093/jac/dkt088.
13. Tada T, Miyoshi-Akiyama T, Dahal RK, Sah MK, Ohara H, Kirikae T, Pokhrel BM. 2013. NDM-8 metallo-beta-lactamase in a multidrugresistant Escherichia coli strain isolated in Nepal. Antimicrob. Agents Chemother. 57:2394-2396. http://dx.doi.org/10.1128/AAC.02553-12.
14. Mochon AB, Garner OB, Hindler JA, Krogstad P, Ward KW, Lewinski MA, Rasheed JK, Anderson KF, Limbago BM, Humphries RM. 2011. New Delhi metallo-lactamase (NDM-1)-producing Klebsiella pneumoniae: case report and laboratory detection strategies. J. Clin. Microbiol. 49:1667-1670. http://dx.doi.org/10.1128/JCM.00183-11.
15. Girlich D, Poirel L, Nordmann P. 2012. Value of the modified Hodge test for detection of emerging carbapenemases in Enterobacteriaceae. J. Clin. Microbiol. 50:477-479. http://dx.doi.org/10.1128/JCM.05247-11.
16. Nordmann P, Poirel L, Dortet L. 2012. Rapid detection of carbapenemase-producing Enterobacteriaceae. Emerg. Infect. Dis. 18:1503-1507. http://dx.doi.org/10.3201/eid1809.120355.
17. Diene SM, Bruder N, Raoult D, Rolain JM. 2011. Real-time PCR assay allows detection of the New Delhi metallo-beta-lactamase (NDM-1)-encoding gene in France. Int. J. Antimicrob. Agents 37:544-546. http://dx.doi.org/10.1016/j. ijantimicag.2011.02.006.
18. Liu W, Zou D, Li Y, Wang X, He X, Wei X, Shao C, Li X, Shang W, Yu K, Liu D, Guo J, Yin Z, Yuan J. 2012. Sensitive and rapid detection of the new Delhi metallo-beta-lactamase gene by loop-mediated isothermal am-plification. J. Clin. Microbiol. 50:1580-1585. http://dx.doi.org/10.1128/JCM.06647-11.
19. Naas T, Ergani A, Carrer A, Nordmann P. 2011. Real-time PCR for detection of NDM-1 carbapenemase genes from spiked stool samples. Antimicrob. Agents Chemother. 55:4038-4043. http://dx.doi.org/10.1128/AAC.01734-10.
20. Cuzon G, Naas T, Bogaerts P, Glupczynski Y, Nordmann P. 2012. Evaluation of a DNA microarray for the rapid detection of extendedspectrum beta-lactamases (TEM, SHV and CTX-M), plasmid-mediated cephalosporinases (CMY-2-like, DHA, FOX, ACC-1, ACT/MIR and CMY-1-like/MOX) and carbapenemases (KPC, OXA-48, VIM, IMP and NDM). J. Antimicrob. Chemother. 67:1865-1869. http://dx.doi.org/10.1093/jac/ dks156.
21. Vasoo S, Cunningham SA, Kohner PC, Mandrekar JN, Lolans K, Hayden MK, Patel R. 2013. Rapid and direct real-time detection of blaKPC and blaNDM from surveillance samples. J. Clin. Microbiol. 51:3609-3615. http://dx.doi.org/10. 1128/JCM.01731-13.
22. Yang YM, Ye HF, Zhang WH, Chen HL, Zhou XM. 2011. Detection of New Delhi metallo-lactamase 1 gene in Klebsiella ozaenae and Acinetobacter baumannii. Int. J. Lab. Med. 32:1407-1409 (In Chinese.)
23. Zhou XM, Liu D, Huang HL. 2011. NDM-1(-) bacteria screening using capillary PCR and direct PCR amplification method. Int. J. Lab. Med. 32:1404-1406 (In Chinese.)
24. Clinical and Laboratory Standards Institute. 2012. Performance standards for antimicrobial susceptibility testing: 22nd informational supplement. CLSI document M100-S22. CLSI, Wayne, PA.
25. Bartual SG, Seifert H, Hippler C, Luzon MA, Wisplinghoff H, Rodriguez-Valera F. 2005. Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii. J. Clin. Microbiol. 43:4382-4390. http://dx.doi.org/10.1128/JCM.43.9.4382-4390.2005. (Pubitemid 41298217)
26. Adams-Haduch JM, Paterson DL, Sidjabat HE, Pasculle AW, Potoski BA, Muto CA, Harrison LH, Doi Y. 2008. Genetic basis of multidrug resistance in Acinetobacter baumannii clinical isolates at a tertiary medical center in Pennsylvania. Antimicrob. Agents Chemother. 52:3837-3843. http://dx.doi.org/10. 1128/AAC.00570-08.
27. Zhang C, Yuan J.-f., Shi Z.-l. 2007. Molecular epidemiological investigation of infectious hypodermal and hematopoietic necrosis virus and taura syndrome virus in Penaeus vannamei cultured in China. Virologica Sinica 22:380-388. http://dx.doi.org/10.1007/s12250-007-0036-x. (Pubitemid 350066051)
28. Akbergenov R, Zhanybekova S, Kryldakov RV, Zhigailov A, Polimbetova NS, Hohn T, Iskakov BK. 2004. ARC-1, a sequence element complementary to an internal 18S rRNA segment, enhances translation efficiency in plants when present in the leader or intercistronic region of mRNAs. Nucleic Acids Res. 32:239-247. http://dx.doi.org/10.1093/nar/gkh176. (Pubitemid 38228622)
29. Zhou M, Geng X, Chen J, Wang X, Wang D, Deng J, Zhang Z, Wang W, Zhang XE, Wei H. 2011. Rapid colorimetric testing for pyrazinamide susceptibility of M. tuberculosis by a PCR-based in-vitro synthesized pyrazinamidase method. PLoS One 6:e27654. http://dx.doi.org/10.1371/journal.pone.0027654.
30. Liang Z, Li L, Wang Y, Chen L, Kong X, Hong Y, Lan L, Zheng M, Guang-Yang C, Liu H, Shen X, Luo C, Li KK, Chen K, Jiang H. 2011. Molecular basis of NDM-1, a new antibiotic resistance determinant. PLoS One 6:e23606. http://dx.doi.org/10.1371/journal.pone.0023606.
31. Barczak AK, Gomez JE, Kaufmann BB, Hinson ER, Cosimi L, Borowsky ML, Onderdonk AB, Stanley SA, Kaur D, Bryant KF, Knipe DM, Sloutsky A, Hung DT. 2012. RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities. Proc. Natl. Acad. Sci. U. S. A. 109:6217-6222. http://dx.doi.org/10.1073/pnas.1119540109.
32. Geyer CN, Reisbig MD, Hanson ND. 2012. Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC-lactamase genes. J. Clin. Microbiol. 50:3722-3725. http://dx.doi.org/10.1128/JCM.02038-12.
33. Pollini S, Maradei S, Pecile P, Olivo G, Luzzaro F, Docquier JD, Rossolini GM. 2013. FIM-1, a new acquired metallo-beta-lactamase from a Pseudomonas aeruginosa clinical isolate from Italy. Antimicrob. Agents Chemother. 57:410-416. http://dx.doi.org/10.1128/AAC.01953-12.