Real-time PCR for detection of NDM-1 carbapenemase genes from spiked stool samples

Antimicrobial Agents and Chemotherapy

Volumn 55, Issue 9, 2011, Pages 4038-4043

  Naas, Thierry ^a,b   Ergani, Ayla ^a,b   Carred̈r, Ameĺie ^a,b   Nordmann, Patrice ^a,b  

^a BICÊTRE HOSPITAL   (France)

^b ASSISTANCE PUBLIQUE HÔPITAUX DE PARIS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; BETA LACTAM; CARBAPENEMASE; NDM 1 PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENOME; BACTERIUM DETECTION; CITROBACTER FREUNDII; COLONY FORMING UNIT; CONTROLLED STUDY; CROSS REACTION; CULTURE MEDIUM; DILUTION; DNA EXTRACTION; ENTEROBACTER CLOACAE; ENTEROBACTERIACEAE; ESCHERICHIA COLI; FECES ANALYSIS; GENE AMPLIFICATION; GENETIC ANALYSIS; GENOTYPE; KLEBSIELLA PNEUMONIAE; LIMIT OF DETECTION; MINIMUM INHIBITORY CONCENTRATION; MULTIDRUG RESISTANCE; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEUS MIRABILIS; PROVIDENCIA STUARTII; PSEUDOMONAS AERUGINOSA; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SERRATIA MARCESCENS; VALIDATION PROCESS;

BACTERIAL PROTEINS; BETA-LACTAMASES; FECES; HUMANS; PLASMIDS; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 80051816745     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.01734-10     Document Type: Article

References (33)

1. Birkett, C I, et al. 2007. Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum β-lactamases. J. Med. Microbiol. 56:52-55. (Pubitemid 46036275)
2. Bisiklis, A., F. Papageorgiou, F. Frantzidou, and S. Alexiou-Daniel. 2007. Specific detection of blaVIM and blaIMP metallo-beta-lactamase genes in a single real-time PCR. Clin. Microbiol. Infect. 13:1201-1203. (Pubitemid 350056126)
3. Bush, K. 2010. Alarming β-lactamase-mediated resistance in multidrugresistant Enterobacteriaceae. Curr. Opin. Microbiol. 13:558-564.
4. Carre¨r, A., N. Fortineau, and P. Nordmann. 2010. Use of ChromID ESBL medium for detecting carbapenemase-producing Enterobacteriaceae. J. Clin. Microbiol. 48:1913-1914.
5. Clinical and Laboratory Standards Institute. 2010. Performance standards for antimicrobial susceptibility testing. CLSI M100-S20U. Update June 2010. Clinical and Laboratory Standards Institute, Wayne, PA.
6. Cole, J M, A N Schuetz, C E Hill, and F S Nolte. 2009. Development and evaluation of a real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase genes. J. Clin. Microbiol. 47:322-326.
7. Cuzon, G., T. Naas, A. Lesenne, M. Benhamou, and P. Nordmann. 2010. Plasmid-mediated carbapenem-hydrolysing OXA-48 beta-lactamase in Klebsiella pneumoniae from Tunisia. Int. J. Antimicrob. Agents 36:91-93.
8. KPC carbapenemase genes by real-time PCR. J. Clin. Microbiol. 46:2879-2883.
9. Kaase, M., et al. 2011. NDM-2 carbapenemase in Acinetobacter baumannii from Egypt. J. Antimicrob. Chemother. 66:1260-1262.
10. Kumarasamy, K K, et al. 2010. Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: A molecular, biological, and epidemiological study. Lancet Infect. Dis. 10:597-602.
11. Livermore, D M, et al. 2007. CTX-M: Changing the face of ESBLs in Europe. J. Antimicrob. Chemother. 59:165-174. (Pubitemid 47072053)
12. Mendes, R E, et al. 2007. Rapid detection and identification of metallobeta- lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis. J. Clin. Microbiol. 45:544-547.
13. Miriagou, V., et al. 2010. Acquired carbapenemases in Gram-negative bacterial pathogens: Detection and surveillance issues. Clin. Microbiol. Infect. 16:112-122.
14. Naas, T., G. Cuzon, H. Truong, S. Bernabeu, and P. Nordmann. 2010. Evaluation of a DNA microarray, the check-points ESBL/KPC array, for rapid detection of TEM, SHV, and CTX-M extended-spectrum beta-lactamases and KPC carbapenemases. Antimicrob. Agents Chemother. 54:3086-3092.
15. Naas, T., C. Oxacelay, and P. Nordmann. 2007. Identification of CTX-M-type extended-spectrum-β-lactamase genes using real-time PCR and pyrosequencing. Antimicrob. Agents Chemother. 51:223-230. (Pubitemid 46047710)
16. Naas, T., L. Poirel, and P. Nordmann. 2008. Minor extended-spectrum β-lactamases. Clin. Microbiol. Infect. 14(Suppl. 1):42-52.
17. Nordmann, P., G. Cuzon, and T. Naas. 2009. The real threat of KPC carbapenemase- producing bacteria. Lancet Infect. Dis. 9:321-331.
18. Nordmann, P., L. Poirel, A. Carre¨r, M. A. Toleman, and T. R. Walsh. 2011. How to detect NDM-1 producers. J. Clin. Microbiol. 49:718-721.
19. Oxacelay, C., A. Ergani, T. Naas, and P. Nordmann. 2007. Rapid detection of CTX-M-producing Enterobacteriaceae in urines samples. J. Antimicrob. Chemother. 64:986-989.
20. Paterson, D L, and R A Bonomo. 2005. Extended-spectrum β-lactamases: A clinical update. Clin. Microbiol. Rev. 18:657-686.
21. Pitout, J D, N. Hamilton, D L Church, P. Nordmann, and L. Poirel. 2007. Development and clinical validation of a molecular diagnostic assay to detect CTX-M-type β-lactamases in Enterobacteriaceae. Clin. Microbiol. Infect. 13:291-297. (Pubitemid 46603360)
22. Poirel, L., E. Lagrutta, P. Taylor, J. Pham, and P. Nordmann. 2010. Emergence of metallo-β-lactamase NDM-1-producing multidrug-resistant Escherichia coli in Australia. Antimicrob. Agents Chemother. 54:4914-4916.
23. Poirel, L., et al. 2011. Extremely drug-resistant Citrobacter freundii isolate producing NDM-1 and other carbapenemases identified in a patient returning from India. Antimicrob. Agents Chemother. 55:447-448.
24. Queenan, A M, and K. Bush. 2007. Carbapenemases: The versatile β-lactamases. Clin. Microbiol. Rev. 20:440-458.
25. Randegger, C C, and H. Hachler. 2001. Real-time PCR and melting curve analysis for reliable and rapid detection of SHV extended-spectrum β-lactamases. Antimicrob. Agents Chemother. 45:1730-1736. (Pubitemid 32466341)
26. Réglier-Poupet, H., et al. 2008. Performance of ChromID ESBL, a chromogenic medium for detection of Enterobacteriaceae producing extended-spectrum β-lactamases. J. Med. Microbiol. 57:310-315. (Pubitemid 351358533)
27. Rodriguez-Martinez, J M, P. Nordmann, N. Fortineau, and L. Poirel. 2010. VIM-19, a metallo-beta-lactamase with Increased carbapenemase activity from Escherichia coli and Klebsiella pneumoniae. Antimicrob. Agents Chemother. 54:471-476.
28. Sambrook, J, and D W Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. Samra, Z., et al. 2008. Evaluation of CHROMagar KPC for rapid detection of carbapenem-resistant Enterobacteriaceae. J. Clin. Microbiol. 46:3110-3111.
30. Walsh, T R 2010. Emerging carbapenemases: A global perspective. Int. J. Antimicrob. Agents 36:S8-S14.
31. Woodford, N., E J Fagan, and M J Ellington. 2006. Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum β-lactamases. J. Antimicrob. Chemother. 57:154-155. (Pubitemid 43057077)
32. Woodford, N., et al. 2010. Comparison of BD Phoenix, Vitek2, and Microscan automated systems for detection and inference of mechanisms responsible for carbapenem resistance in Enterobacteriaceae. J. Clin. Microbiol. 48:2999-3002.
33. NDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob. Agents Chemother. 53:5046-5054.