Library generation by gene shuffling

Current Protocols in Molecular Biology

Volumn , Issue SUPPL.105, 2013, Pages

  Meyer, Adam J ^a   Ellefson, Jared W ^a   Ellington, Andrew D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Directed evolution; PCR; Recombination

Indexed keywords

DEOXYRIBONUCLEASE I; DOUBLE STRANDED DNA; PLASMID DNA; DNA FRAGMENT;

ARTICLE; DNA SEQUENCE; GENE AMPLIFICATION; GENE LIBRARY; GENE SHUFFLING; HOMOLOGOUS RECOMBINATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; CLINICAL PROTOCOL; DNA PURIFICATION; GENE MUTATION; GENE SEQUENCE; GENETIC PARAMETERS; GENETIC SCREENING; GENETIC SELECTION; MOLECULAR EVOLUTION;

EID: 84901611709     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb1512s105     Document Type: Article

References (14)

1. Abécassis, V., Pompon, D., and Truan, G. 2000. High efficiency family shuffling based on multistep PCR and in vivo DNA recombination in yeast: Statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2. Nucleic Acids Res. 28:E88.
2. Bershtein, S., Goldin, K., and Tawfik, D.S. 2008. Intense neutral drifts yield robust and evolvable consensus proteins. J. Mol. Biol. 379:1029-1044.
3. Brühlmann, F. and Chen,W. 1999. Tuning biphenyl dioxygenase for extended substrate specificity. Biotechnol. Bioeng. 63:544-551.
4. Farrow, M.F. and Arnold, F.H. 2010. Combinatorial recombination of gene fragments to construct a library of chimeras. Curr. Protoc. Prot. Sci. 26.2.1-26.2.20.
5. Giver, L., Gershenson, A., Freskgard, P.O., and Arnold, F.H. 1998. Directed evolution of a thermostable esterase. Proc. Natl. Acad. Sci. U.S.A. 95:12809-12813.
6. Hiraga, K. and Arnold, F.H. 2003. General method for sequence-independent site-directed chimeragenesis. J. Mol. Biol. 330:287-296.
7. Jézéquel, L., Loeper, J., and Pompon, D. 2008. Sequence-independent construction of ordered combinatorial libraries with predefined crossover points. BioTechniques 45:523-532.
8. Joern, J.M., Meinhold, P., and Arnold, F.H. 2002. Analysis of shuffled gene libraries. J. Mol. Biol. 316:643-656.
9. Lorimer, I.A. and Pastan, I. 1995. Random recombination of antibody single chain Fv sequences after fragmentation with DNaseI in the presence of Mn2+. Nucleic Acids Res. 23:3067-3068.
10. Maheshri, N., Koerber, J.T., Kaspar, B.K., and Schaffer, D.V 2006. Directed evolution of adeno-associated virus yields enhanced gene delivery vectors. Nat. Biotechnol. 24:198-204.
11. Miyazaki, K. and Arnold, F.H. 1999. Exploring nonnatural evolutionary pathways by saturation mutagenesis: Rapid improvement of protein function. J. Mol. Evol. 49:716-720.
12. Pekrun, K., Shibata, R., Igarashi, T., Reed, M., Sheppard, L., Patten, P.A., Stemmer, W.P.C., Martin, M.A., and Soong, N.-W. 2002. Evolution of a human immunodeficiency virus type 1 variant with enhanced replication in pigtailed macaque cells by DNA shuffling. J. Virol. 76:2924-2935.
13. Stemmer, W.P. 1994. Rapid evolution of a protein in vitro by DNA shuffling. Nature 370:389-391.
14. Zhao, H. and Arnold, F.H. 1997. Optimization of DNA shuffling for high fidelity recombination. Nucleic Acids Res. 25:1307-1308.