Structural and functional characterization of the chaperone Hsp70 from sugarcane. Insights into conformational changes during cycling from cross-linking/mass spectrometry assays

Journal of Proteomics

Volumn 104, Issue , 2014, Pages 48-56

  Tiroli Cepeda, Ana O ^a,b   Lima, Tatiani B ^a   Balbuena, Tiago S ^c   Gozzo, Fábio C ^a   Ramos, Carlos H I ^a,b  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagem   (Brazil)

^c SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

Heat shock protein; Hsp70; Molecular chaperone; Protein dynamics; Protein folding; Sugarcane

Indexed keywords

CHAPERONE; HEAT SHOCK PROTEIN 40; HEAT SHOCK PROTEIN 70; RECOMBINANT PROTEIN; SSHSP70 PROTEIN; UNCLASSIFIED DRUG; PROTEIN BINDING;

ARTICLE; BIOGEOCHEMICAL CYCLING; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DNA LIBRARY; DNA SEQUENCE; ENZYME ACTIVITY; EXPRESSED SEQUENCE TAG; EXPRESSION VECTOR; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN CROSS LINKING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTEIN UNFOLDING; RESTRICTION SITE; STRUCTURE ANALYSIS; SUGARCANE; WESTERN BLOTTING; AMINO ACID SEQUENCE; BINDING SITE; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; ENZYME ACTIVATION; ENZYMOLOGY; MOLECULAR GENETICS; PROCEDURES; ULTRASTRUCTURE;

SACCHARUM;

AMINO ACID SEQUENCE; BINDING SITES; COMPUTER SIMULATION; ENZYME ACTIVATION; HSP70 HEAT-SHOCK PROTEINS; MASS SPECTROMETRY; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN CONFORMATION; SACCHARUM;

EID: 84901590538     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2014.02.004     Document Type: Article

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