A Spectroscopic-based Laboratory Experiment for Protein Conformational Studies

Biochemistry and Molecular Biology Education

Volumn 32, Issue 1, 2004, Pages 31-34

  Ramos, Carlos Henrique I ^a,b  

^a LABORATÓRIO NACIONAL DE LUZ SÍNCROTRON   (Brazil)

^b UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

Circular dichroism; Fluorescence; Laboratory class; Protein folding and stability

Indexed keywords

ANALYTIC METHOD; ARTICLE; BIOCHEMISTRY; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; FLUORESCENCE; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STABILITY; SPECTROPHOTOMETRY; SPECTROSCOPY; TEACHING; TECHNIQUE;

EID: 1642576024     PISSN: 14708175     EISSN: None     Source Type: Journal    
DOI: 10.1002/bmb.2004.494032010309     Document Type: Article

References (11)

1. T. E. Creighton (1997) Protein Folding, 1st Ed., W. H. Freeman and Company, New York.
2. P. S. Kim, R. L. Baldwin (1990) Intermediates in the folding reactions of small proteins, Annu. Rev. Biochem. 59, 631-660.
3. C. R. Cantor, P. R. Shimmel (1980) Biophysical Chemistty, 1st Ed., W. H. Freeman and Company, San Francisco.
4. H. Edelhoch (1967) Spectroscopic determination of tryptophan and tyrosine in proteins, Biochemistry 6, 1948-1954.
5. T. Takano (1977) Structure of myoglobin refined at 2.0 Å resolution I. Crystallographic refinement of metmyoglobin from sperm whale. J. Mol. Biol. 110, 537-568.
6. S. V. Evans, G. D. Brayer (1988) Horse heart metmyoglobin. A 2.8-A resolution three-dimensional structure determination. J. Biol. Chem. 263, 4263-4268.
7. J. R. Lakowicz (1999) Principles of Fluorescence Spectroscopy, 2nd Ed., Kluwer Academic Publishers, Dordrecht, The Netherlands.
8. M. R. Eftink. (1994) The use of fluorescence methods to monitor unfolding transitions in proteins. Biophys. J. 66, 482-501.
9. G. D. Fasman (1996) Circular Dichroism and the Conformational Analysis of Biomolecules, 1st Ed., Plenum Press, New York.
10. C. H. I. Ramos, M. S., Kay, R. L. Baldwin (1999) Putative ion pairs involved in myoglobin stability, Biochemistry 38, 9783-9790.
11. C. H. I. Ramos, R. L. Baldwin (2002) Sulfate anion stabilization of native ribonuclease A both by anion binding and by the Hofmeister effect. Protein Sci. 11, 1771-1778.