Simultaneous expression of multiple proteins under a single promoter in Caenorhabditis elegans via a versatile 2A-based toolkit

Genetics

Volumn 196, Issue 3, 2014, Pages 605-613

  Ahier, Arnaud ^a   Jarriault, Sophie ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

[No Author keywords available]

Indexed keywords

HISTONE; PEPTIDE; POLYPEPTIDE; UNCLASSIFIED DRUG; VIRAL 2A PEPTIDE;

ANIMAL EXPERIMENT; ARTICLE; BIOLOGICAL ACTIVITY; CAENORHABDITIS ELEGANS; CELL TYPE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DEVELOPMENTAL STAGE; DNA SEQUENCE; DRUG DELIVERY SYSTEM; ENZYME RECONSTITUTION; GENE EXPRESSION; GENE SEQUENCE; GENETIC ANALYSIS; INTRACELLULAR SPACE; MOLECULAR BIOLOGY; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FUNCTION; WORM;

2A PEPTIDE; CAENORHABDITIS ELEGANS; INTERNAL RIBOSOMAL ENTRY SITE (IRES); SL2; STOICHIOMETRIC DELIVERY OF MULTIPLE POLYPEPTIDES;

ANIMALS; CAENORHABDITIS ELEGANS; GENE EXPRESSION; GENETIC TECHNIQUES; GENETIC VECTORS; PEPTIDES; PROMOTER REGIONS, GENETIC; RIBOSOMES; TRANSFECTION; VIRUSES;

EID: 84901343283     PISSN: 00166731     EISSN: 19432631     Source Type: Journal    
DOI: 10.1534/genetics.113.160846     Document Type: Article

References (35)

1. Atkins, J. F., N. M. Wills, G. Loughran, C. Y. Wu, K. Parsawar et al., 2007 A case for "StopGo": reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go). RNA 13: 803-810.
2. Burglin, T. R., and G. Ruvkun, 2001 Regulation of ectodermal and excretory function by the C. elegans POU homeobox gene ceh-6. Development 128: 779-790.
3. Carey, B. W., S. Markoulaki, J. Hanna, K. Saha, Q. Gao et al., 2009 Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc. Natl. Acad. Sci. USA 106: 157-162.
4. Chen, L., Y. Fu, M. Ren, B. Xiao, and C. S. Rubin, 2011 A RasGRP, C. elegans RGEF-1b, couples external stimuli to behavior by activating LET-60 (Ras) in sensory neurons. Neuron 70: 51-65.
5. de Felipe, P., 2002 Polycistronic viral vectors. Curr. Gene Ther. 2: 355-378.
6. Diao, F., and B. H. White, 2012 A novel approach for directing transgene expression in Drosophila: T2A-Gal4 in-frame fusion. Genetics 190: 1139-1144.
7. Dickinson, D. J., J. D. Ward, D. J. Reiner, and B. Goldstein, 2013 Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Nat. Methods 10: 1028-1034.
8. Doronina, V. A., C. Wu, P. de Felipe, M. S. Sachs, M. D. Ryan et al., 2008 Site-specific release of nascent chains from ribosomes at a sense codon. Mol. Cell. Biol. 28: 4227-4239.
9. El Amrani, A., A. Barakate, B. M. Askari, X. Li, A. G. Roberts et al., 2004 Coordinate expression and independent subcellular targeting of multiple proteins from a single transgene. Plant Physiol. 135: 16-24.
10. Fang, J., J. J. Qian, S. Yi, T. C. Harding, G. H. Tu et al., 2005 Stable antibody expression at therapeutic levels using the 2A peptide. Nat. Biotechnol. 23: 584-590.
11. Felix, M. A., A. Ashe, J. Piffaretti, G. Wu, I. Nuez et al., 2011 Natural and experimental infection of Caenorhabditis nematodes by novel viruses related to nodaviruses. PLoS Biol. 9: e1000586.
12. Franz, C. J., G. Zhao, M. A. Felix, and D. Wang, 2012 Complete genome sequence of Le Blanc virus, a third Caenorhabditis nematodeinfecting virus. J. Virol. 86: 11940.
13. Gonzalez, M., I. Martin-Ruiz, S. Jimenez, L. Pirone, R. Barrio et al., 2011 Generation of stable Drosophila cell lines using multicistronic vectors. Sci. Rep. 1: 75.
14. Hahn, H., and A. C. Palmenberg, 1996 Mutational analysis of the encephalomyocarditis virus primary cleavage. J. Virol. 70: 6870-6875.
15. Hajduskova, M., A. Ahier, T. Daniele, and S. Jarriault, 2012 Cell plasticity in Caenorhabditis elegans: from induced to natural cell reprogramming. Genesis 50: 1-17.
16. Hasegawa, K., A. B. Cowan, N. Nakatsuji, and H. Suemori, 2007 Efficient multicistronic expression of a transgene in human embryonic stem cells. Stem Cells 25: 1707-1712.
17. Jarriault, S., Y. Schwab, and I. Greenwald, 2008 A Caenorhabditis elegans model for epithelial-neuronal transdifferentiation. Proc. Natl. Acad. Sci. USA 105: 3790-3795.
18. Kagias, K., A. Ahier, N. Fischer, and S. Jarriault, 2012 Members of the NODE (Nanog and Oct4-associated deacetylase) complex and SOX-2 promote the initiation of a natural cellular reprogramming event in vivo. Proc. Natl. Acad. Sci. USA 109: 6596-6601.
19. Kim, J. H., S. R. Lee, L. H. Li, H. J. Park, J. H. Park et al., 2011 High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. PLoS ONE 6: e18556.
20. Lee, K. K., Y. Gruenbaum, P. Spann, J. Liu, and K. L. Wilson, 2000 C. elegans nuclear envelope proteins emerin, MAN1, lamin, and nucleoporins reveal unique timing of nuclear envelope breakdown during mitosis. Mol. Biol. Cell 11: 3089-3099.
21. Li, D., and M. Wang, 2012 Construction of a bicistronic vector for the co-expression of two genes in Caenorhabditis elegans using a newly identified IRES. Biotechniques 52: 173-176.
22. Liu, X., F. Long, H. Peng, S. J. Aerni, M. Jiang et al., 2009 Analysis of cell fate from single-cell gene expression profiles in C. elegans. Cell 139: 623-633.
23. Luke, G. A., P. de Felipe, A. Lukashev, S. E. Kallioinen, E. A. Bruno et al., 2008 Occurrence, function and evolutionary origins of '2A-like' sequences in virus genomes. J. Gen. Virol. 89: 1036-1042.
24. Macosko, E. Z., N. Pokala, E.H. Feinberg, S. H. Chalasani, R. A. Butcher et al., 2009 A hub-and-spoke circuit drives pheromone attraction and social behaviour in C. elegans. Nature 458: 1171-1175.
25. Miyazaki, K., 2011 MEGAWHOP cloning: a method of creating random mutagenesis libraries via megaprimer PCR of whole plasmids. Methods Enzymol. 498: 399-406.
26. Pfleger, B. F., D. J. Pitera, C. D. Smolke, and J. D. Keasling, 2006 Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes. Nat. Biotechnol. 24: 1027-1032.
27. Provost, E., J. Rhee, and S. D. Leach, 2007 Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos. Genesis 45: 625-629.
28. Richard, J. P., S. Zuryn, N. Fischer, V. Pavet, N. Vaucamps et al., 2011 Direct in vivo cellular reprogramming involves transition through discrete, non-pluripotent steps. Development 138: 1483-1492.
29. Rieckher, M., N. Kourtis, A. Pasparaki, and N. Tavernarakis, 2009 Transgenesis in Caenorhabditis elegans. Methods Mol. Biol. 561: 21-39.
30. Szymczak, A. L., and D. A. Vignali, 2005 Development of 2A peptide-based strategies in the design of multicistronic vectors. Expert Opin. Biol. Ther. 5: 627-638.
31. Szymczak, A. L., C. J. Workman, Y. Wang, K. M. Vignali, S. Dilioglou et al., 2004 Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nat. Biotechnol. 22: 589-594.
32. Torres, V., L. Barra, F. Garces, K. Ordenes, S. Leal-Ortiz et al., 2010 A bicistronic lentiviral vector based on the 1D/2A sequence of foot-and-mouth disease virus expresses proteins stoichiometrically. J. Biotechnol. 146: 138-142.
33. Tursun, B., L. Cochella, I. Carrera, and O. Hobert, 2009 A toolkit and robust pipeline for the generation of fosmid-based reporter genes in C. elegans. PLoS ONE 4: e4625.
34. Zuryn, S., S. Le Gras, K. Jamet, and S. Jarriault, 2010 A strategy for direct mapping and identification of mutations by wholegenome sequencing. Genetics 186: 427-430.
35. Zuryn, S., T. Daniele, and S. Jarriault, 2012 Direct cellular reprogramming in Caenorhabditis elegans: facts, models, and promises for regenerative medicine. Wiley Interdiscip. Rev. Dev. Biol. 1: 138-152.