Mechanically and chemically tunable cell culture system for studying the myofibroblast phenotype

Langmuir

Volumn 30, Issue 19, 2014, Pages 5481-5487

  Saums, Michele K ^a   Wang, Weifeng ^a   Han, Biao ^b   Madhavan, Lakshmi ^a   Han, Lin ^b   Lee, Daeyeon ^a   Wells, Rebecca G ^c  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b DREXEL UNIVERSITY   (United States)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMIMETICS; COLLAGEN; HYDROGELS; STIFFNESS MATRIX; TISSUE; TISSUE ENGINEERING;

COMBINED EFFECT; CULTURE SYSTEMS; ENVIRONMENTAL FACTORS; HEPATIC STELLATE CELLS; LAYER-BY-LAYERS; MECHANICAL FORCE; MECHANICAL STIFFNESS; PROOF OF CONCEPT;

CELL CULTURE;

HYDROGEL;

ANIMAL; CULTURE TECHNIQUE; CYTOLOGY; HUMAN; HYDROGEL; MYOFIBROBLAST; PROCEDURES;

ANIMALS; CELL CULTURE TECHNIQUES; HUMANS; HYDROGELS; MYOFIBROBLASTS;

EID: 84901044572     PISSN: 07437463     EISSN: 15205827     Source Type: Journal    
DOI: 10.1021/la4047758     Document Type: Article

References (31)

1. Discher, D. E.; Janmey, P.; Wang, Y. L. Tissue cells feel and respond to the stiffness of their substrate Science 2005, 310 (5751) 1139-43
2. Stroka, K. M.; Konstantopoulos, K. Theme: Physical Biology in Cancer. 4. Physical cues guide tumor cell adhesion and migration. Am. J. Physiol.: Cell Physiol. 2013, doi: 10.1152/ajpcell.00289.2013.
3. Engler, A. J.; Sen, S.; Sweeney, H. L.; Discher, D. E. Matrix elasticity directs stem cell lineage specification Cell 2006, 126 (4) 677-89
4. Liu, F.; Mih, J. D.; Shea, B. S.; Kho, A. T.; Sharif, A. S.; Tager, A. M.; Tschumperlin, D. J. Feedback amplification of fibrosis through matrix stiffening and COX-2 suppression J. Cell Biol. 2010, 190 (4) 693-706
5. Olsen, A. L.; Bloomer, S. A.; Chan, E. P.; Gaca, M. D.; Georges, P. C.; Sackey, B.; Uemura, M.; Janmey, P. A.; Wells, R. G. Hepatic stellate cells require a stiff environment for myofibroblastic differentiation Am. J. Physiol.: Gastrointest. Liver Physiol. 2011, 301 (1) G110-8
6. Li, Z.; Dranoff, J. A.; Chan, E. P.; Uemura, M.; Sevigny, J.; Wells, R. G. Transforming growth factor-beta and substrate stiffness regulate portal fibroblast activation in culture Hepatology 2007, 46 (4) 1246-1256
7. Missirlis, D.; Spatz, J. P. Combined Effects of PEG Hydrogel Elasticity and Cell-Adhesive Coating on Fibroblast Adhesion and Persistent Migration Biomacromolecules 2014, 15, 195-205
8. Chen, A. A.; Khetani, S. R.; Lee, S.; Bhatia, S. N.; Van Vliet, K. J. Modulation of hepatocyte phenotype in vitro via chemomechanical tuning of polyelectrolyte multilayers Biomaterials 2009, 30 (6) 1113-1120
9. Mhanna, R. F.; Voros, J.; Zenobi-Wong, M. Layer-by-layer films made from extracellular matrix macromolecules on silicone substrates Biomacromolecules 2011, 12 (3) 609-616
10. Gribova, V.; Gauthier-Rouviere, C.; Albiges-Rizo, C.; Auzely-Velty, R.; Picart, C. Effect of RGD functionalization and stiffness modulation of polyelectrolyte multilayer films on muscle cell differentiation Acta Biomater. 2013, 9 (5) 6468-6480
11. Schneider, A.; Bolcato-Bellemin, A. L.; Francius, G.; Jedrzejwska, J.; Schaaf, P.; Voegel, J. C.; Frisch, B.; Picart, C. Glycated polyelectrolyte multilayer films: differential adhesion of primary versus tumor cells Biomacromolecules 2006, 7 (10) 2882-2889
12. Gaudiere, F.; Masson, I.; Morin-Grognet, S.; Thoumire, O.; Vannier, J. P.; Atmani, H.; Ladam, G.; Labat, B. Mechano-chemical control of cell behaviour by elastomer templates coated with biomimetic layer-by-layer nanofilms Soft Matter 2012, 8 (32) 8327-8337
13. Yamanlar, S.; Sant, S.; Boudou, T.; Picart, C.; Khademhosseini, A. Surface functionalization of hyaluronic acid hydrogels by polyelectrolyte multilayer films Biomaterials 2011, 32 (24) 5590-5599
14. Friedman, S. L. Hepatic fibrosis - overview Toxicology 2008, 254 (3) 120-129
15. Chakravarti, S.; Petroll, W. M.; Hassell, J. R.; Jester, J. V.; Lass, J. H.; Paul, J.; Birk, D. E. Corneal opacity in lumican-null mice: defects in collagen fibril structure and packing in the posterior stroma Invest. Ophthalmol. Visual Sci. 2000, 41 (11) 3365-3373
16. Krishnan, A.; Li, X.; Kao, W. Y.; Viker, K.; Butters, K.; Masuoka, H.; Knudsen, B.; Gores, G.; Charlton, M. Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis Lab. Invest. 2012, 92 (12) 1712-1725
17. Thompson, M. T.; Berg, M. C.; Tobias, I. S.; Rubner, M. F.; Van Vliet, K. J. Tuning compliance of nanoscale polyelectrolyte multilayers to modulate cell adhesion Biomaterials 2005, 26 (34) 6836-6845
18. Flanagan, L. A.; Ju, Y. E.; Marg, B.; Osterfield, M.; Janmey, P. A. Neurite branching on deformable substrates Neuroreport 2002, 13 (18) 2411-2415
19. Pelham, R. J., Jr.; Wang, Y. Cell locomotion and focal adhesions are regulated by substrate flexibility Proc. Natl. Acad. Sci. U.S.A. 1997, 94 (25) 13661-13665
20. Yeung, T.; Georges, P. C.; Flanagan, L. A.; Marg, B.; Ortiz, M.; Funaki, M.; Zahir, N.; Ming, W.; Weaver, V.; Janmey, P. A. Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion Cell Motil. Cytoskel. 2005, 60 (1) 24-34
21. Pullela, S. R.; Andres, C.; Chen, W.; Xu, C.; Wang, L.; Kotov, N. A. Permselectivity Replication of Artificial Glomerular Basement Membranes in Nanoporous Collagen Multilayers J. Phys. Chem. Lett. 2011, 2 (16) 2067-2072
22. Hutter, J. L.; Bechhoefer, J. Calibration of Atomic-Force Microscope Tips Rev. Sci. Instrum. 1993, 64 (7) 1868-1873
23. Han, L.; Frank, E. H.; Greene, J. J.; Lee, H. Y.; Hung, H. H.; Grodzinsky, A. J.; Ortiz, C. Time-dependent nanomechanics of cartilage Biophys. J. 2011, 100 (7) 1846-1854
24. Ahearne, M.; Yang, Y.; El Haj, A. J.; Then, K. Y.; Liu, K. K. Characterizing the viscoelastic properties of thin hydrogel-based constructs for tissue engineering applications J. R. Soc., Interface 2005, 2 (5) 455-463
25. Mott, P. H.; Dorgan, J. R.; Roland, C. M. The bulk modulus and Poissons ratio of incompressible materials J. Sound Vib. 2008, 312 (4-5) 572-575
26. Dimitriadis, E. K.; Horkay, F.; Maresca, J.; Kachar, B.; Chadwick, R. S. Determination of elastic moduli of thin layers of soft material using the atomic force microscope Biophys. J. 2002, 82 (5) 2798-2810
27. Conover, W. J.; Iman, R. L. Rank Transformations as a Bridge between Parametric and Nonparametric Statistics-Rejoinder Am. Stat. 1981, 35 (3) 132-133
28. Braet, F.; De Zanger, R.; Wisse, E. Drying cells for SEM, AFM and TEM by hexamethyldisilazane: a study on hepatic endothelial cells J. Microsc. 1997, 186 (Pt 1) 84-87
29. Uemura, M.; Swenson, E. S.; Gaca, M. D.; Giordano, F. J.; Reiss, M.; Wells, R. G. Smad2 and Smad3 play different roles in rat hepatic stellate cell function and alpha-smooth muscle actin organization Mol. Biol. Cell 2005, 16 (9) 4214-4224
30. Kim, D. H.; Provenzano, P. P.; Smith, C. L.; Levchenko, A. Matrix nanotopography as a regulator of cell function J. Cell Biol. 2012, 197 (3) 351-360
31. Wang, L.; Carrier, R. L. Biomimetic Topography: Bioinspired Cell Culture Substrates and Scaffolds. In Adv. Biomimetics [Online]; Cavrak, M., Ed.; 2011; http://www.intechopen.com/books/advances-in-biomimetics/biomimetic-topography- bioinspired-cell-culture-substrates-and-scaffolds.