Thermostable lipase from Geobacillus sp. Iso5: Bioseparation, characterization and native structural studies

Journal of Basic Microbiology

Volumn 54, Issue 5, 2014, Pages 386-396

  Mahadevan, Gurumurthy D ^a   Neelagund, Shivayogeeswar E ^a  

^a KUVEMPU UNIVERSITY   (India)

Author keywords

Circular dichromism; FTIR and Raman spectroscopy; Geobacillus; Lipase; MALDI TOF MS MS

Indexed keywords

GEOBACILLUS;

ENZYME ACTIVATOR; ENZYME INHIBITOR; THERMOSTABLE LIPASE; TRIACYLGLYCEROL LIPASE;

CHEMISTRY; CIRCULAR DICHROISM; ENZYME STABILITY; ENZYMOLOGY; GEOBACILLUS; HUMAN; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SECONDARY STRUCTURE; TANDEM MASS SPECTROMETRY; TEMPERATURE;

CHROMATOGRAPHY, LIQUID; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATORS; ENZYME INHIBITORS; ENZYME STABILITY; GEOBACILLUS; HUMANS; HYDROGEN-ION CONCENTRATION; LIPASE; MOLECULAR WEIGHT; PROTEIN STRUCTURE, SECONDARY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TANDEM MASS SPECTROMETRY; TEMPERATURE;

EID: 84900470753     PISSN: 0233111X     EISSN: 15214028     Source Type: Journal    
DOI: 10.1002/jobm.201200656     Document Type: Article

References (63)

1. Haki, G.D., Rakshit, S.K., 2003. Developments in industrially important thermostable enzymes: a review. Biores. Technol., 89, 17-34.
2. Bruins, M.E., Janssenm A.E., Boom, R.M., 2001. Thermozymes and their applications: a review of recent literature and patents. Appl. Biochem. Biotechnol., 90, 155-186.
3. Jaeger, K.E., Schneidinger, B., Rosenau, F., Werner, M. et al., 1997. Bacterial lipases for biotechnological applications. J. Mol. Catal. B: Enzym., 3, 3-12.
4. Ghosh, P.K., Saxena, R.K., Rani, G., Yadav, R.P. et al., 1996. Microbial lipases: production and applications. Sci. Prog., 2, 119-157.
5. Jaeger, K.E., Reetz, M.T., 1998. Microbial lipases form versatile tools for biotechnology. Trends. Biotechnol., 16, 396-403.
6. Singh, R., Gupta, N., Goswami, V.K., Gupta, R., 2006. A simple activity staining protocol for lipases and esterases. Appl. Microbiol. Biotechnol., 70, 679-682.
7. Hassan, F., Shah, A.A., Hameed, A., 2006. Industrial applications of microbial lipases. Enzyme. Micro. Techol., 39, 235-251.
8. Schmidt, D., Sztajer, H., Stocklein, W, Menge, U. et al., 1994. Screening purification and properties of a thermophilic lipase from Bacillus thermocatenulatus. Biochim. Biophys. Acta, 1214, 43-53.
9. Jaeger, K.E., Ransac, S., Dijkstra, B.W., Colson, C. et al., 1994. Bacterial lipases. FEMS. Microbiol. Rev., 15, 29-63.
10. Leow, T.C., Rahman, R.N., Basri, M., Salleh, A.B., 2007. A thermoalklophilic lipase of Geobacillus sp. T1. Extremophiles, 11, 527-535.
11. Dominfuez, A., Pastrana, L., Longo, M.A., Rua, M.L. et al., 2005. Lipolytic enzyme production by Thermus thermophiles HB27 in a stirred tank bioreactor. Biochem. Eng. J., 26, 95-99.
12. Vieille, C., Zeikus, G.J., 2001. Hyperthermophilic enzymes: sources, uses and molecular mechanisms for thermostability. Microbiol. Mol. Biol. Rev., 65, 1-43.
13. Becker, P., Reesh, I.A., Markossian, S., Antranikian, G. et al., 1997. Determination of the kinetic parameters during continuous cultivation of the lipase-producing thermophile Bacillus sp. I HI-91 on olive oil. Appl. Microbiol. Biotechnol., 48, 184-190.
14. Gomes, J., Steiner, W., 2004. The biocatalytic potential of extremophiles and extremozymes. Food. Technol. Biotechnol., 42, 223-235.
15. Van den Burg, B., 2003. Extremophiles as a source for novel enzymes. Curr. Opin. Microbiol., 6, 213-218.
16. Sharma, R., Chisti, Y., Banerjee, U.C., 2001. Production, purification, characterization and applications of lipases. Biotechnol. Adv., 19, 627-662.
17. Pandey, A., Benjamin, S., Scoccol, C.R., Nigam, P. et al., 1999. The realm of microbial lipases in biotechnology. Biotechnol. Appl. Biochem., 29, 119-131.
18. Sharma, R., Soni, S.K., Vohra, R.M., Gupta, L.K. et al., 2002. Purification and characterization of a thermostable alkaline lipase from a new thermophilic Bacillus sp. RSJ-1R. Process. Biochem., 37, 1075-1084.
19. Daniel, R.M., Danson, M.J., Eisenthal, R., Lee, C.K. et al., 2008. The effects of temperature on enzyme activity: new insights and their implications. Extremophiles, 12, 51-56.
20. Stetter, K.O., 2006. Hyperthermophiles in the history of life. Phil. Trans. Roy. Soc. Biol. Sci., 361, 1837-1843.
21. Li, H., Zhang, X., 2005. Characterization of thermostable lipase from thermophilic Geobacillus sp. TW1. Prot. Expr. Purif., 42, 153-159.
22. Kim, H.K., Park, S.Y., Lee, J.K., Oh, T.K., 1998. Gene cloning and characterization of thermostable lipase from Bacillus stearothermophilus L1. Biosci. Biotechnol. Biochem., 62, 66-71.
23. Soliman, N.A., Knoll, M., Abdel-Fattah, Y.R., Schmid, R.D. et al., 2007. Molecular cloning and characterization of thermostable esterase and lipase from Geobacillus thermoleovorans YN isolated from desert soil in Egypt. Process. Biochem., 42, 1090-1100.
24. Quintana-Castro, R., Diaz, P., Valerio-Alfaro, G., Gracia, H.S., Oliart-Ros, R., 2009. Gene cloning, expression, and characterization of the Geobacillus Thermoleovorans CCR11 thermoalklophilic lipase. Mol. Biotechnol., 42, 75-83.
25. Gurumurthy, D.M., Neelagund, S.E., 2010. Geobacillus sp Iso 5, a novel amylase producing thermophile from konkan region thermal springs in southern India. J. Earth. Sci., 21, 318-322.
26. Nazina, T.N., Tourova, T.P., Poltaraus, A.B., Novikova, E.V. et al., 2001. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans. Int. J. Syst. Evol. Microbiol., 51, 433-446.
27. McMullan, G., Christe, J.M., Rahman, T.J., Banat, I.M. et al., 2004. Habitat, application and genomics of the aerobic, thermophilic genus Geobacillus. Biochem. Soci. Trans., 32, 214-217.
28. Abd Rahman, R.N., Leow, T.C., Salleh, A.B., Basri, M., 2007. Geobacillus zalihae sp. nov., a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia. BMC. Microbiol., 7, 77.
29. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., 1951. Protein estimation with the Folin phenol reagent. J. Biol. Chem., 193, 265-275.
30. Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature, 227, 680-685.
31. Ma, J., Zhang, Z., Wang, B., Kong, X. et al., 2006. Overexpression and characterization of a lipase from Bacillus subtilis. Protein. Expr. Purif., 45, 22-29.
32. Greenfield, N.J., 2006. Using circular dichroism spectra to estimate protein secondary structure. Nature Protocols, 1, 2876-2890.
33. Daspupta, S., Rousseau, D.L., Anni, H., Yonetani, T., 1989. Structural characterization of cytochrome c peroxidase by resonance Raman scattering. J. Biol. Chem., 264(1), 654-662.
34. Sassaroli, M., Dasgupta, S., Rousseau, D.L., 1986. Cryogenic stabilization of myoglobin photoproducts. J. Bol. Chem., 261, 13704-13713.
35. Han, X.X., Kitahama, Y., Tanaka, Y., Guo, J. et al., 2008. Simplified protocol for detection of protein-ligand interactions via surface-enhanced resonance raman Scattering and surface-enhanced fluorescence. Anal. Chem., 80, 6567-6572.
36. Royter, M., Schmidt, M., Elend, C., Hobenreich, H. et al., 2009. Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOL1 and Caldanaerobacter subterraneus subsp. Tengcongensis. Extremophiles, 13, 769-783.
37. Arpigny, J.L., Jaeger, K.E., 1999. Bacterial lipolytic enzymes: classification and properties. Biochem, J., 343, 177-183.
38. Jaeger, K.E., Eggert, T., 2002. Lipases for biotechnology. Curr. Opin. Biotechnol., 13, 390-397.
39. Sanchez-Otero, M.G., Ruiz-López, I.I., Avila-Nieto, D.E., Oliart-Ros, R.M., 2011. Significant improvement of Geobacillus thermoleovorans CCR11 thermoalklophilic lipase production using response surface methodology. New Biotechnol., 28(6), 761-766.
40. Rodriguez, J.A., Mateos, J.C., Nungaray, J., Gonzalez, V. et al., 2006. Improving lipase production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation. Process. Biochem., 44, 2264-2269.
41. Joseph, B., Ramteke, P.W., Thomas, G., Shrivastava, N., 2007. Standard review cold-active microbial lipases: a versatile tool for industrial applications. Biotechnol. Mol. Biol. Rev., 2, 39-48.
42. Ribeiro, B.D., de Castro, A.M., Coelho, M.A., Freire, D.M., 2011. Production and use of lipases in bioenergy: a review from the feedstock to biodiesel production. Enzyme. Res., 1-16.
43. Treichel, H., Oliveira de, D., Mazutti, M.A., Luccio Di, M. et al., 2010. A review on microbial lipases production. Food. Bioprocess. Technol., 3, 182-196.
44. Sinchaikul, S., Sookkheo, B., Phutrakul, S., Pan, F.M. et al., 2001. Optimization of a thermostable lipase from Bacillus stearothermophilus P1: overexpression, purification and characterization. Prot. Expr. Purif., 22, 388-398.
45. Abdel-Fattah, Y.R., Gaballa, A.A., 2008. Identification and over-expression of a thermostable lipase from Geobacillus thermoleovorans Toshki in Escherichia coli. Microbiol. Res., 163, 13-20.
46. Saxena, R.K., Sheoran, A., Giri, B., Davidson, W.S., 2003. Purification strategies for microbial lipases. J. Microbiol. Meth., 52, 1-18.
47. Lee, D., Koh, Y., Kim, K., Kim, B. et al., 1999. Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1. FEMS Microbiol. Lett., 179, 393-400.
48. Palomo, J.M., Segura, R.L., Fernández-Lorente, G., Pernas, M. et al., 2004. Purification, immobilization, and stabilization of a lipase from Bacillus thermocatenulatus by interfacial adsorption on hydrophobic supports. Biotechnol. Progress., 20, 630-635.
49. Nawani, N., Kaur, J., 2000. Purification, characterization and thermostability of a lipase from a thermophilic Bacillus sp. J33. Mol. Cell. Biochem., 206, 91-96.
50. Rahman, R.N., Chin, J.H., Salleh, A.B., Basri, M., 2003. Cloning and expression of a novel lipase gene from Bacillus sphaericus 205y. Mol. Genet. Genomics., 269, 252-260.
51. Schmidt-Dannert, C., Rua, M.L., Atomi, H., Schnmid, R.D., 1996. Thermoalkalophilic lipase of Bacillus thermocatenulatus. I. Molecular cloning, nucleotide sequence, purification and some properties. Biochim. Biophys. Acta, 1301, 105-114.
52. Castro-Ochoa, L.D., Rodriguez-Gomez, C., Valerio-Alfaro, G., Ros, R.O., 2005. Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11. Enzyme. Micro. Technol., 37, 648-654.
53. Lesuisse, E., Schanck, K., Colson, C., 1993. Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme. Eur. J. Biochem., 216, 155-160.
54. Sunna, A., Tokajian, S., Burghardt, J., Rainey, F. et al., 1997. Identification of Bacillus kaustophilus, Bacillus thermocatenulatus and Bacillus strain HSR as members of Bacillus thermolerovorans. Syst. Appl. Microbiol., 20, 232-237.
55. Arora, P.K., 2012. Staphylococcus lipolyticus sp. nov., a new cold adapted lipase producing marine species. Ann. Microbiol., DOI: 10.1007/s13213-012-0544-2
56. Kim, K.R., Kwon, D.Y., Yoon, S.H., Kim, W.Y. et al., 2005. Purification, refolding and characterization of recombinant Pseudomonas fluorescens lipase. Prot. Expr. Purif., 39, 124-129.
57. Nardini, M., Lang, D.A., Liebeton, K., Jaeger, K.E. et al., 2000. Crystal structure of Pseudomonas aeruginosa lipase in the open conformation. The prototype for family I.1 of bacterial lipase. J. Biol. Chem., 275, 31219-31225.
58. Bowden, M.G., Visai, L., Longshaw, C.M., Holland, K.T. et al., 2002. Is the GehD lipase from Staphylococcus epidermidis a collagen binding adhesion? J. Biol. Chem., 277, 43017-43023.
59. Wharton, C.W., 1986. Infrared and Raman spectroscopic studies of enzyme structure and function. Biochem. J., 233, 25-36.
60. Smulevich, G., Paoli, M., Burke, J.F., Sanders, S.A. et al., 1994. Characterization of recombinant horseradish peroxidase C and three site-directed mutants, F41V, F41W, and R38K, by resonance raman spectroscopy. Biochemistry, 33, 7398-7407.
61. Carey, P.R., Tonge, P.J., 1995. Unlocking the secrets of enzyme power using raman spectroscopy. Ace. Chem. Res., 28, 8-13.
62. Benevides, J.M., Overman, S.A., Thomas, G.J., 2004. Raman spectroscopy of proteins. Curr. Protoc. Prot. Sci., 17.8.1-17.8.35.
63. Das, R.S., Agarwal, Y.K., 2011. Raman spectroscopy: recent advancements, techniques and applications. Vibrational Spec., 57, 163-176.