Mitochondrial calcium uniporter activity is dispensable for MDA-MB-231 breast carcinoma cell survival

PLoS ONE

Volumn 9, Issue 5, 2014, Pages

  Hall, Duane D ^a   Wu, Yuejin ^a   Domann, Frederick E ^a   Spitz, Douglas R ^a   Anderson, Mark E ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRUS VECTOR; CALCIUM ION; CARRIER PROTEIN; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE; MITOCHONDRIAL CALCIUM UNIPORTER; MITOCHONDRIAL PROTEIN; PROTEIN MICU1; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; ANTINEOPLASTIC AGENT; CALCIUM; CALCIUM BINDING PROTEIN; CALCIUM CHANNEL; CATION TRANSPORT PROTEIN; MICU1 PROTEIN, HUMAN; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL; ARTICLE; BREAST CARCINOMA; BREAST EPITHELIUM; CALCIUM TRANSPORT; CANCER CELL LINE; CARCINOMA CELL; CELL KILLING; CELL STIMULATION; CELL STRESS; CELL SURVIVAL; CLONOGENESIS; CONTROLLED STUDY; ENZYME PHOSPHORYLATION; EPITHELIUM CELL; GENE SILENCING; HUMAN; HUMAN CELL; MDA MB 231 CELL; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; OUTCOME ASSESSMENT; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN SUBUNIT; WILD TYPE; ALGORITHM; ANTAGONISTS AND INHIBITORS; BREAST TUMOR; CHEMISTRY; DRUG EFFECTS; FEMALE; GENETICS; HELA CELL LINE; IONIZING RADIATION; METABOLISM; MITOCHONDRION; PATHOLOGY; RADIATION RESPONSE; RNA INTERFERENCE; TUMOR CELL LINE;

ALGORITHMS; AMP-ACTIVATED PROTEIN KINASES; ANTINEOPLASTIC AGENTS; BREAST NEOPLASMS; CALCIUM; CALCIUM CHANNELS; CALCIUM-BINDING PROTEINS; CATION TRANSPORT PROTEINS; CELL LINE, TUMOR; CELL SURVIVAL; FEMALE; HELA CELLS; HUMANS; MITOCHONDRIA; MITOCHONDRIAL MEMBRANE TRANSPORT PROTEINS; RADIATION, IONIZING; REACTIVE OXYGEN SPECIES; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EID: 84900451895     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0096866     Document Type: Article

References (31)

1. Baughman JM, Perocchi F, Girgis HS, Plovanich M, Belcher-Timme CA, et al. (2011) Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter. Nature 476: 341-345. doi:10.1038/nature10234.
2. De Stefani D, Raffaello A, Teardo E, Szabò I, Rizzuto R (2011) A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter. Nature: 1-24. doi:10.1038/nature10230.
3. 2+ Uniporter. Cell Metab 17: 976-987. doi:10.1016/j.cmet.2013.04.020.
4. 2+ Uptake that Regulates Cell Survival. Cell 151: 630-644. doi:10.1016/j.cell.2012.10.011.
5. 2+ uptake. Nature 467: 291-296. doi:10.1038/nature09358.
6. 2 mediate the differential susceptibility of cancer cells versus normal cells to glucose deprivation. Biochem J 418: 29. doi:10.1042/BJ20081258.
7. Fantin VR, St-Pierre J, Leder P (2006) Attenuation of LDH-A expression uncovers a link between glycolysis, mitochondrial physiology, and tumor maintenance. Cancer Cell 9: 425-434. doi:10.1016/j.ccr.2006.04.023. (Pubitemid 43832190)
8. Denton RM (2009) Regulation of mitochondrial dehydrogenases by calcium ions. BBA - Bioenergetics 1787: 1309-1316. doi:10.1016/j.bbabio.2009.01.005.
9. 2+ in the Regulation of Cellular Energetics. Biochemistry 51: 2959-2973. doi:10.1021/bi2018909.
10. Marchi S, Lupini L, Patergnani S, Rimessi A, Missiroli S, et al. (2012) Downregulation of the Mitochondrial Calcium Uniporter by Cancer-Related miR-25. Curr Biol. doi:10.1016/j.cub.2012.11.026.
11. Madden SF, Clarke C, Aherne ST, Gaule P, O'Donovan N, et al. (2013) BreastMark: an integrated approach to mining publicly available transcriptomic datasets relating to breast cancer outcome. Breast Cancer Res 15: R52. doi:10.1186/bcr3444.
12. Imanishi H, Hattori K, Wada R, Ishikawa K, Fukuda S, et al. (2011) Mitochondrial DNA mutations regulate metastasis of human breast cancer cells. PLoS ONE 6: e23401. doi:10.1371/journal.pone.0023401.
13. Owens KM, Kulawiec M, Desouki MM, Vanniarajan A, Singh KK (2011) Impaired OXPHOS Complex III in Breast Cancer. PLoS ONE 6: e23846. doi:10.1371/journal. pone.0023846.t001.
14. Curry MC, Peters AA, Kenny PA, Roberts-Thomson SJ, Monteith GR (2013) Mitochondrial calcium uniporter silencing potentiates caspase-independent cell death in MDA-MB-231 breast cancer cells. Biochem Biophys Res Commun. doi:10.1016/j.bbrc.2013.04.015.
15. Nagai T, Sawano A, Park ES, Miyawaki A (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc Natl Acad Sci USA 98: 3197-3202. doi:10.1073/pnas.051636098. (Pubitemid 32220822)
16. 2+ Indicators Based on Computationally Redesigned Calmodulin-Peptide Pairs. Chem Biol 13: 521-530. doi:10.1016/j.chembiol.2006.03. 007.
17. 2-resistant cell lines. Do aldehydic by-products of lipid peroxidation contribute to oxidative stress? Biochem J 267: 453-459.
18. Cai Z, Chattopadhyay N, Liu WJ, Chan C, Pignol J-P, et al. (2011) Optimized digital counting colonies of clonogenic assays using ImageJ software and customized macros: comparison with manual counting. Int J Radiat Biol 87: 1135-1146. doi:10.3109/09553002.2011.622033.
19. Dahle J, Kakar M, Steen HB, Kaalhus O (2004) Automated counting of mammalian cell colonies by means of a flat bed scanner and image processing. Cytometry 60A: 182-188. doi:10.1002/cyto.a.20038. (Pubitemid 39079511)
20. Joiner M-LA, Koval OM, Li J, He BJ, Allamargot C, et al. (2012) CaMKII determines mitochondrial stress responses in heart. Nature. doi:10.1038/ nature11444.
21. Lee WJ, Monteith GR, Roberts-Thomson SJ (2006) Calcium transport and signaling in the mammary gland: targets for breast cancer. Biochim Biophys Acta.
22. Denton RM, McCormack JG (1980) The role of calcium in the regulation of mitochondrial metabolism. Biochem Soc Trans 8: 266-268.
23. 2+ concentration of the endoplasmic reticulum is a key determinant of ceramide-induced apoptosis: significance for the molecular mechanism of Bcl-2 action. EMBO J 20: 2690-2701. doi:10.1093/emboj/20.11.2690.
24. 2-mediated oxidative stress. Free Radic Biol Med 48: 1024-1033. doi:10.1016/j. freeradbiomed.2010.01.018.
25. Qiu J, Tan Y-W, Hagenston AM, Martel M-A, Kneisel N, et al. (2013) Mitochondrial calcium uniporter Mcu controls excitotoxicity and is transcriptionally repressed by neuroprotective nuclear calcium signals. Nat Commun 4: 2034. doi:10.1038/ncomms3034.
26. Pan X, Liu J, Nguyen T, Liu C, Sun J, et al. (2013) The physiological role of mitochondrial calcium revealed by mice lacking the mitochondrial calcium uniporter. Nat Cell Biol. doi:10.1038/ncb2868.
27. Raffaello A, De Stefani D, Sabbadin D, Teardo E, Merli G, et al. (2013) The mitochondrial calcium uniporter is a multimer that can include a dominant-negative pore-forming subunit. EMBO J. doi:10.1038/emboj.2013.157.
28. 2+ uptake that regulates cellular metabolism. Nat Cell Biol. doi:10.1038/ncb2622.
29. Sancak Y, Markhard AL, Kitami T, Kovács-Bogdán E, Kamer KJ, et al. (2013) EMRE is an Essential Component of the Mitochondrial Calcium Uniporter Complex. Science. doi:10.1126/science.1242993.
30. Plovanich M, Bogorad RL, Sancak Y, Kamer KJ, Strittmatter L, et al. (2013) MICU2, a Paralog of MICU1, Resides within the Mitochondrial Uniporter Complex to Regulate Calcium Handling. PLoS ONE 8: e55785. doi:10.1371/journal. pone.0055785.
31. 2+ Uniporter by Exerting Opposite Effects on MCU Activity. Mol Cell. doi:10.1016/j.molcel. 2014.01.013.