Automated counting of mammalian cell colonies by means of a flat bed scanner and image processing

Cytometry Part A

Volumn 60, Issue 2, 2004, Pages 182-188

  Dahle, Jostein ^a   Kakar, Manish ^a   Steen, Harald B ^a   Kaalhus, Olav ^a  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

Automated colony counting; Flatbed scanner; Mammalian cells

Indexed keywords

ACCURACY; ALGORITHM; ANIMAL CELL; ARTICLE; AUTOMATION; CELL KILLING; CLONOGENIC ASSAY; CONTROLLED STUDY; DEVICE; FLAT BED SCANNER; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IMAGE PROCESSING; IRRADIATION; MAMMAL CELL; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; STANDARD; VIDEORECORDER;

EID: 4043178290     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.20038     Document Type: Article

References (11)

1. Lumley MA, Burgess R, Billingham LJ, McDonald DF, Milligan DW. Colony counting is a major source of variation in CFU-GM results between centres. Br J Haematol 1997;97:481-484.
2. Salmon SE, Young L, Lebowitz J, Thomson S, Einsphar J, Tong T, Moon TE. Evaluation of an automated image analysis system for counting human tumor colonies. Int J Cell Cloning 1984;2:142-160.
3. Perceptive Instruments. www.perceptive.co.uk. 2002.
4. Oxford Optronix. www.oxford-optronix.com. 2002.
5. Dobson K, Reading L, Scutt A. A cost-effective method for the automatic quantitative analysis of fibroblastic colony-forming units. Calcif Tissue Int 1999;65:166-172.
6. Barber PR, Vojnovic B, Kelly J, Mayes CR, Boulton P, Woodcock M, Joiner MC. Automated counting of mammalian cell colonies. Phys Med Biol 2001;46:63-76.
7. Thielmann HW, Hagedorn R, Freber W. Evaluation of colony-forming ability, experiments using normal and DNA repair-deficient human fibroblast strains and an automatic colony counter. Cytometry 1985; 6:130-136.
8. Boukamp P, Petrussevska RT, Breitkreutz D, Hornung J, Markham A, Fusenig NE. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 1988;106:761-771.
9. Dahle J, Kvam E. Induction of delayed mutations and chromosomal instability in fibroblasts after UVA-, UVB- and X-radiation. Cancer Res 2003;63:1464-1469.
10. Puck TT, Marcus PI. Action of X-rays on mammalian cells. J Exp Med 1956;103:653-66.
11. Gonzalez RC, Woods RE. Digital image processing. Reading, MA: Addison-Wesley; 1992.