The Ca2+ concentration of the endoplasmic reticulum is a key determinant of ceramide-induced apoptosis: Significance for the molecular mechanism of Bcl-2 action

EMBO Journal

Volumn 20, Issue 11, 2001, Pages 2690-2701

  Pinton, Paolo ^a   Ferrari, Davide ^b   Rapizzi, Elena ^b   Di Virgilio, Francesco ^b   Pozzan, Tullio ^a   Rizzuto, Rosario ^b  

^a UNIVERSITY OF PADOVA   (Italy)

^b Section of General Pathology   (Italy)

Author keywords

Apoptosis; Bcl 2; Ca2+; Ceramide; Mitochondria

Indexed keywords

CALCIUM ION; CALRETICULIN; CERAMIDE; PROTEIN BCL 2; ADENOSINE TRIPHOSPHATASE (CALCIUM); AEQUORIN; CALCIUM; CALCIUM BINDING PROTEIN; CASP3 PROTEIN, HUMAN; CASPASE; CASPASE 3; DRUG DERIVATIVE; ENZYME INHIBITOR; GREEN FLUORESCENT PROTEIN; N ACETYLSPHINGOSINE; N-ACETYLSPHINGOSINE; PHOTOPROTEIN; RECOMBINANT PROTEIN; RIBONUCLEOPROTEIN; SARCOPLASMIC RETICULUM CALCIUM TRANSPORTING ADENOSINE TRIPHOSPHATASE; SPHINGOSINE;

APOPTOSIS; ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CONTROLLED STUDY; CYTOSOL; ENDOPLASMIC RETICULUM; GENE CONTROL; GENE OVEREXPRESSION; HELA CELL; HUMAN; HUMAN CELL; MITOCHONDRIAL MEMBRANE; MOLECULAR DYNAMICS; ONCOGENE; PRIORITY JOURNAL; DRUG EFFECT; GENETIC TRANSFECTION; GENETICS; KINETICS; METABOLISM; MITOCHONDRION; PHYSIOLOGY; PROTO ONCOGENE; REPORTER GENE;

AEQUORIN; APOPTOSIS; CALCIUM; CALCIUM-BINDING PROTEINS; CALCIUM-TRANSPORTING ATPASES; CALRETICULIN; CASPASE 3; CASPASES; ENDOPLASMIC RETICULUM; ENZYME INHIBITORS; GENES, BCL-2; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; KINETICS; LUMINESCENT PROTEINS; MITOCHONDRIA; PROTO-ONCOGENE PROTEINS C-BCL-2; RECOMBINANT PROTEINS; RIBONUCLEOPROTEINS; SARCOPLASMIC RETICULUM CALCIUM-TRANSPORTING ATPASES; SPHINGOSINE; TRANSFECTION;

EID: 0035355341     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.11.2690     Document Type: Article

References (67)

1. 2+] in the endoplasmic reticulum and cytoplasm of intact HeLa cells. A comparative study
2. 2+ stores and reveals aspects of their lumenal microenvironment and function
3. The mitochondrial permeability transition
4. The versatility and universality of calcium signalling
5. Bcl-2 and Bcl-2-related proteins in apoptosis regulation
6. c). A critical evaluation
7. 2+ signalling
8. SERCA1 splice variants encoding C-terminally truncated proteins reduce ER calcium content and induce apoptosis
9. BCL-2 family: Regulators of cell death
10. The mitochondrial permeability transition pore
11. Quasi-synaptic calcium signal transmission between endoplasmic reticulum and mitochondria
12. Contributions of mitochondria to animal physiology: From homeostatic sensor to calcium signalling and cell death
13. Mechanisms and functions of cell death
14. The purinergic P2Z receptor of human macrophage cells: Characterization and possible physiological role
15. Delayed activation of the store-operated calcium current induced by calreticulin overexpression in RBL-1 cells
16. 2+ concentration within the endoplasmic reticulum
17. 2+ indicators with greatly improved fluorescence properties
18. Ceramide in the eukaryotic stress response
19. Maintenance of calcium homeostasis in the endoplasmic reticulum by Bcl-2
20. Programmed cell death in Caenorhabditis elegans
21. To die or not to die: An overview of apoptosis and its role in disease
22. Blockade of NMDA receptors and apoptotic neurodegeneration in the developing brain
23. Mitochondrial membrane permeabilization during the apoptotic process
24. Differential modulation of SERCA2 isoforms by calreticulin
25. 2+ pool
26. The pro-apoptotic proteins, Bid and Bax, cause a limited permeabilization of the mitochondrial outer membrane that is enhanced by cytosol
27. Calreticulin
28. Modulation of endoplasmic reticulum calcium pump by Bcl-2
29. 2+ fluxes
30. Deciphering the pathways of life and death
31. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade
32. The protein product of the oncogene bcl-2 is a component of the nuclear envelope, the endoplasmic reticulum and the outer mitochondrial membrane
33. Signal transduction of stress via ceramide
34. Apoptotic and anti-apoptotic synaptic signaling mechanisms
35. 2+ influx
36. L forms an ion channel in synthetic lipid membranes
37. Apoptosis by death factor
38. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β
39. Changes in endoplasmic reticulum luminal environment affect cell sensitivity to apoptosis
40. Caspase structure, proteolytic substrates and function during apoptotic cell death
41. Neuronal cell death: A demise with different shapes
42. Chromatin condensation during apoptosis is accompanied by degradation of lamin A+B, without enhanced activation of cdc2 kinase
43. Separate pools of endonuclease activity are responsible for internucleosomal and high molecular mass DNA fragmentation during apoptosis
44. 2+ movements and equilibration
45. Ceramide and apoptosis
46. Disruption of the outer mitochondrial membrane as a result of large amplitude swelling: The impact of irreversible permeability transition
47. 2+ store, with functional properties distinct from those of the endoplasmic reticulum
48. 2+ influx in Bcl-2-overexpressing cells
49. Lamin proteolysis facilitates nuclear events during apoptosis
50. GD3 ganglioside directly targets mitochondria in a bcl-2-controlled fashion
51. 2+ revealed by specifically targeted recombinant aequorin
52. 2+] in mitochondria of living cells
53. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells
54. Digital imaging microscopy of living cells
55. 2+ responses
56. Channel formation by antiapoptotic protein Bcl-2
57. Calpain and calpastatin regulate neutrophil apoptosis
58. Bcl-2-mediated drug resistance: Inhibition of apoptosis by blocking nuclear factor of activated T lymphocytes (NFAT)-induced Fas ligand transcription
59. Molecular characterization of mitochondrial apoptosis-inducing factor
60. Mitochondrial release of caspase-2 and -9 during the apoptotic process
61. 3-linked mitochondrial calcium signals
62. Bcl-xL regulates the membrane potential and volume homeostasis of mitochondria
63. Endonuclease activities associated with high molecular weight and internucleosomal DNA fragmentation in apoptosis
64. 2+-induced apoptosis through calcineurin dephosphorylation of BAD
65. 2+ store depletion
66. Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked
67. Bcl-2 interrupts the ceramide-mediated pathway of cell death