Vitrification of Tokuyasu-style immuno-labelled sections for correlative cryo light microscopy and cryo electron tomography

Journal of Structural Biology

Volumn 186, Issue 2, 2014, Pages 273-282

  Bos, Erik ^a   Hussaarts, Leonie ^a   van Weering, Jan R T ^b   Ellisman, Mark H ^c   de Wit, Heidi ^b   Koster, Abraham J ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b VU UNIVERSITY AMSTERDAM   (Netherlands)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Correlative microscopy; Cryo electron tomography; Cryo sections; Immunogold labelling; Synapse; Vitrification

Indexed keywords

ANTIBODY LABELING; ARTICLE; CELL ULTRASTRUCTURE; DENDRITIC CELL; ELECTRON TOMOGRAPHY; HUMAN; HUMAN CELL; IMMUNOGOLD LABELING; MICROSCOPY; NERVE CELL; PRIORITY JOURNAL; TISSUE SECTION; VITRIFICATION; ANIMAL; CRYOELECTRON MICROSCOPY; CYTOLOGY; FLUORESCENCE MICROSCOPY; LYSOSOME; MICROTOMY; MONOCYTE; MOUSE; PHASE CONTRAST MICROSCOPY; PROCEDURES; SYNAPSE; ULTRASTRUCTURE;

ALEXA 488 HYDRAZIDE; HYDRAZINE DERIVATIVE;

ANIMALS; CRYOELECTRON MICROSCOPY; ELECTRON MICROSCOPE TOMOGRAPHY; HUMANS; HYDRAZINES; LYSOSOMES; MICE; MICROSCOPY, FLUORESCENCE; MICROSCOPY, PHASE-CONTRAST; MICROTOMY; MONOCYTES; NEURONS; SYNAPSES; VITRIFICATION;

EID: 84899993414     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2014.03.021     Document Type: Article

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