Absolute quantitation of host cell proteins in recombinant human monoclonal antibodies with an automated CZE-ESI-MS/MS system

Electrophoresis

Volumn 35, Issue 10, 2014, Pages 1448-1452

  Zhu, Guijie ^a   Sun, Liangliang ^a   Linkous, Travis ^b   Kernaghan, Dawn ^b   Mcgivney, James B ^b   Dovichi, Norman J ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

^b MEDIMMUNE   (United States)

Author keywords

CZE; Electrokinetically pumped sheath flow nanospray; Host cell proteins; Recombinant antibodies

Indexed keywords

AMINO ACIDS; ISOTOPES; MONOCLONAL ANTIBODIES; RECOMBINANT PROTEINS;

CZE; ELECTROKINETICALLY PUMPED SHEATH FLOW NANOSPRAY; ESI-MS-MS; HOST CELL PROTEIN; HUMAN MONOCLONAL ANTIBODIES; NANOELECTROSPRAY; NANOSPRAY; PEAKS AREAS; RECOMBINANT ANTIBODY; SHEATH FLOWS;

PEPTIDES;

ADENYLATE KINASE; GLUTATHIONE TRANSFERASE; MONOCLONAL ANTIBODY; RECOMBINANT ANTIBODY; TRANSALDOLASE; PEPTIDE; RECOMBINANT PROTEIN;

ANALYTIC METHOD; ARTICLE; AUTOMATION; CALIBRATION; CAPILLARY ZONE ELECTROPHORESIS; ELECTROSPRAY MASS SPECTROMETRY; ISOTOPE LABELING; MASS SPECTROMETER; NUCLEOTIDE SEQUENCE; PROTEIN ISOLATION; QUANTITATIVE ANALYSIS; SAMPLER; SEPARATION TECHNIQUE; TANDEM MASS SPECTROMETRY; AMINO ACID SEQUENCE; CAPILLARY ELECTROPHORESIS; CHEMISTRY; HUMAN; MOLECULAR GENETICS; PROCEDURES;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; ELECTROPHORESIS, CAPILLARY; HUMANS; MOLECULAR SEQUENCE DATA; PEPTIDES; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY;

EID: 84899906874     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.201300545     Document Type: Article

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