Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding

Protein Science

Volumn 20, Issue 7, 2011, Pages 1145-1154

  Magalhães, Maria L B ^a   Czekster, Clarissa Melo ^a   Guan, Rong ^a   Malashkevich, Vladimir N ^a   Almo, Steven C ^a   Levy, Matthew ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Dissociation kinetics; In vitro compartmentalization; Loop; Streptavidin

Indexed keywords

BIOTIN; LIGAND; STREPTAVIDIN;

ARTICLE; CONTROLLED STUDY; DISSOCIATION; HUMAN; LIGAND BINDING; MUTANT; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN VARIANT; WASTE;

BINDING SITES; BIOTIN; CALORIMETRY; CRYSTALLOGRAPHY, X-RAY; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; STREPTAVIDIN; STREPTOMYCES; SURFACE PLASMON RESONANCE; TITRIMETRY;

EID: 79959372166     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.642     Document Type: Article

References (31)

1. Weber PC, Ohlendorf DH, Wendoloski JJ, Salemme FR (1989) Structural origins of high-affinity biotin binding to streptavidin. Science 243:85-88. (Pubitemid 19027930)
2. Piran U, Riordan WJ (1990) Dissociation rate constant of the biotin-streptavidin complex. J Immunol Methods 133:141-143. (Pubitemid 20334494)
3. DeChancie J, Houk KN (2007) The origins of femtomolar protein-ligand binding: hydrogen-bond cooperativity and desolvation energetics in the biotin-(strept)avidin binding site. J Am Chem Soc 129:5419-5429. (Pubitemid 46697629)
4. Green NM (1975) Avidin. Adv Protein Chem 29:85-133.
5. Hendrickson WA, Pahler A, Smith JL, Satow Y, Merritt EA, Phizackerley RP (1989) Crystal structure of core streptavidin determined from multiwavelength anomalous diffraction of synchrotron radiation. Proc Natl Acad Sci USA 86:2190-2194.
6. Hyre DE, Le Trong I, Merritt EA, Eccleston JF, Green NM, Stenkamp RE, Stayton PS (2006) Cooperative hydrogen bond interactions in the streptavidin-biotin system. Protein Sci 15:459-467.
7. Klumb LA, Chu V, Stayton PS (1998) Energetic roles of hydrogen bonds at the ureido oxygen binding pocket in the streptavidin-biotin complex. Biochemistry 37:7657-7663. (Pubitemid 28248623)
8. Chilkoti A, Tan PH, Stayton PS (1995) Site-directed mutagenesis studies of the high-affinity streptavidin-biotin complex: contributions of tryptophan residues 79, 108, and 120. Proc Natl Acad Sci USA 92:1754-1758.
9. Chu V, Freitag S, Le Trong I, Stenkamp RE, Stayton PS (1998) Thermodynamic and structural consequences of flexible loop deletion by circular permutation in the streptavidin-biotin system. Protein Sci 7:848-859. (Pubitemid 28216527)
10. Levy M, Ellington AD (2008) Directed evolution of streptavidin variants using in vitro compartmentalization. Chem Biol 15:979-989.
11. Chivers CE, Crozat E, Chu C, Moy VT, Sherratt DJ, Howarth M A streptavidin variant with slower biotin dissociation and increased mechanostability. Nat Methods 7:391-393.
12. Kurzban GP, Gitlin G, Bayer EA, Wilchek M, Horowitz PM (1990) Biotin binding changes the conformation and decreases tryptophan accessibility of streptavidin. J Protein Chem 9:673-682.
13. Freitag S, Le Trong I, Klumb L, Stayton PS, Stenkamp RE (1997) Structural studies of the streptavidin binding loop. Protein Sci 6:1157-1166. (Pubitemid 27260150)
14. Ebner A, Marek M, Kaiser K, Kada G, Hahn CD, Lackner B, Gruber HJ (2008) Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives. Methods Mol Biol 418:73-88. (Pubitemid 351366012)
15. Wu SC, Wong SL (2005) Engineering soluble monomeric streptavidin with reversible biotin binding capability. J Biol Chem 280:23225-23231.
16. Aslan FM, Yu Y, Mohr SC, Cantor CR (2005) Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein. Proc Natl Acad Sci USA 102:8507-8512. (Pubitemid 40862766)
17. Freitag S, Le Trong I, Klumb LA, Chu V, Chilkoti A, Stayton PS, Stenkamp RE (1999) X-ray crystallographic studies of streptavidin mutants binding to biotin. Biomol Eng 16:13-19. (Pubitemid 30184834)
18. Katz BA (1995) Binding to protein targets of peptidic leads discovered by phage display: crystal structures of streptavidin-bound linear and cyclic peptide ligands containing the HPQ sequence. Biochemistry 34:15421-15429.
19. Korndorfer IP, Skerra A (2002) Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site. Protein Sci 11:883-893. (Pubitemid 34241296)
20. Qureshi MH, Yeung JC, Wu SC, Wong SL (2001) Development and characterization of a series of soluble tetrameric and monomeric streptavidin muteins with differential biotin binding affinities. J Biol Chem 276:46422-46428.
21. Laitinen OH, Hytonen VP, Nordlund HR, Kulomaa MS (2006) Genetically engineered avidins and streptavidins. Cell Mol Life Sci 63:2992-3017. (Pubitemid 46072304)
22. Weber PC, Cox MJ, Salemme FR, Ohlendorf DH (1987) Crystallographic data for Streptomyces avidinii streptavidin. J Biol Chem 262:12728-12729.
23. Miyazaki K (2003) Creating random mutagenesis libraries by megaprimer PCR of whole plasmid (MEGA-WHOP). Methods Mol Biol 231:23-28.
24. Otwinowski ZM, W (1997) Processing of x-ray Diffraction data collected in Oscillation Mode. Methods in Enzymology 276:307-326.
25. Storoni LC, McCoy AJ, Read RJ (2004) Likelihood-enhanced fast rotation functions. Acta Crystallogr D Biol Crystallogr 60:432-438. (Pubitemid 41087570)
26. Breaker RR, Joyce GF (1994) A DNA enzyme that cleaves RNA. Chem Biol 1:223-229. (Pubitemid 124002020)
27. Emsley P, Cowtan K (2004) Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60:2126-2132. (Pubitemid 41742764)
28. Hooft RW, Vriend G, Sander C, Abola EE (1996) Errors in protein structures. Nature 381:272.
29. Laskowski RA, Moss DS, Thornton JM (1993) Mainchain bond lengths and bond angles in protein structures. J Mol Biol 231:1049-1067. (Pubitemid 23209879)
30. Krissinel E, Henrick K (2004) Secondary-structure matching (SSM), a new tool for fast protein structure alignment in three dimensions. Acta Crystallogr D Biol Crystallogr 60:2256-2268. (Pubitemid 41742778)
31. Avrantinis SK, Stafford RL, Tian X, Weiss GA (2002) Dissecting the streptavidin-biotin interaction by phage-displayed shotgun scanning. Chembiochem 3:1229-1234. (Pubitemid 36005315)