Real-time monitoring and control of soluble signaling factors enables enhanced progenitor cell outputs from human cord blood stem cell cultures

Biotechnology and Bioengineering

Volumn 111, Issue 6, 2014, Pages 1258-1264

  Csaszar, Elizabeth ^a,b   Chen, Kun ^a   Caldwell, Julia ^a   Chan, Warren ^a,b,c   Zandstra, Peter W ^a,b,c,d  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

^d UNIVERSITY HEALTH NETWORK   (Canada)

Author keywords

Bioreactor; Hematopoietic stem cells; Process control; Protein detection; Soluble signaling factors

Indexed keywords

BIOREACTORS; BLOOD; CELL CULTURE; CELL PROLIFERATION; FEEDBACK; MEDICAL PROBLEMS; MONITORING; PROCESS CONTROL; PROTEINS; REAL TIME CONTROL; SEMICONDUCTOR QUANTUM DOTS; SIGNALING; STEM CELLS;

CORD BLOOD STEM CELLS; HEMATOPOIETIC PROGENITOR CELLS; HEMATOPOIETIC STEM CELLS; INTEGRATED REAL-TIME CONTROL; MONITORING AND CONTROL; PROTEIN CONCENTRATIONS; PROTEIN DETECTION; SOLUBLE SIGNALING FACTORS;

CELL SIGNALING;

QUANTUM DOT; TRANSFORMING GROWTH FACTOR BETA1; CULTURE MEDIUM;

ARTICLE; BATCH REACTOR; CELL DENSITY; CELL EXPANSION; CELL POPULATION; CONTROLLED STUDY; CULTURE MEDIUM; EX VIVO STUDY; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; MONITORING; PRIMARY CELL CULTURE; PROCESS CONTROL; SIGNAL TRANSDUCTION; STEM CELL CULTURE; UMBILICAL CORD BLOOD; BATCH CELL CULTURE; CELL CULTURE; CHEMISTRY; CULTURE TECHNIQUE; CYTOLOGY; FETUS BLOOD; PHYSIOLOGY; PROCEDURES; STEM CELL; TIME;

BATCH CELL CULTURE TECHNIQUES; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CULTURE MEDIA; FETAL BLOOD; HUMANS; STEM CELLS; TIME FACTORS; TRANSFORMING GROWTH FACTOR BETA1;

EID: 84899646174     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25163     Document Type: Article

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