An automated system for delivery of an unstable transcription factor to hematopoietic stem cell cultures

Biotechnology and Bioengineering

Volumn 103, Issue 2, 2009, Pages 402-412

  Csaszar, Elizabeth ^a,b   Gavigan, Geneviève ^a   Ungrin, Mark ^a   Thérien, Cynthia ^c   Dubé, Pascale ^c   Féthière, James ^d,e   Sauvageau, Guy ^c,d   Roy, Denis Claude ^c   Zandstra, Peter W ^a,b,f  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c MAISONNEUVE ROSEMONT HOSPITAL   (Canada)

^d INSTITUTE FOR RESEARCH IN IMMUNOLOGY AND CANCER   (Canada)

^e UNIVERSITÉ DE MONTRÉAL   (Canada)

^f UNIVERSITY HEALTH NETWORK   (Canada)

Author keywords

Bioreactor; Delivery system; Hematopoietic stem cell; Mathematical model; Reporter assay; TAT HOXB4

Indexed keywords

AUTOMATED DELIVERY SYSTEM; AUTOMATED SYSTEMS; BIOPROCESS; CELL ASSAYS; CONCENTRATION RANGES; DELIVERY SYSTEM; DELIVERY SYSTEMS; DOSING STRATEGY; EXTRACELLULAR; HALF LIVES; HEMATOPOIETIC STEM CELL; HEMATOPOIETIC STEM CELLS; HUMAN PERIPHERAL BLOOD; INTRACELLULAR CONCENTRATION; KINETIC STUDY; LABOR INTENSIVE; OPTIMAL GROWTH; PRIMARY CELL CULTURES; PROGENITOR CELL; PROTEIN CONCENTRATIONS; REPORTER ASSAY; STEM CELL CULTURE; TAT-HOXB4; UNSTABLE MOLECULES; USER INTERVENTION;

ANIMAL CELL CULTURE; AUTOMATION; BIOREACTORS; CELLS; COMPUTER SIMULATION; ENZYME ACTIVITY; INDUSTRIAL CHEMICALS; MATHEMATICAL MODELS; OPTIMIZATION; TRANSCRIPTION; TRANSCRIPTION FACTORS;

CELL CULTURE;

TAT HOXB4 PROTEIN; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ARTICLE; BIOPROCESS; BLOOD SAMPLING; CELL ASSAY; CELL CULTURE; CELL EXPANSION; CELL FATE; CELL GROWTH; ENZYME ACTIVITY; HALF LIFE TIME; HEMATOPOIETIC STEM CELL; HUMAN; KINETICS; MATHEMATICAL MODEL; MOLECULAR DYNAMICS; PREDICTION; PROTEIN TRANSPORT; VALIDATION PROCESS; AUTOMATION; BIOTECHNOLOGY; METABOLISM; METHODOLOGY;

AUTOMATION; BIOTECHNOLOGY; CELLS, CULTURED; HALF-LIFE; HEMATOPOIETIC STEM CELLS; HUMANS; TRANSCRIPTION FACTORS;

EID: 65549085650     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22297     Document Type: Article

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