Characterization of an autotransporter adhesin protein shared by Burkholderia mallei and Burkholderia pseudomallei

BMC Microbiology

Volumn 14, Issue 1, 2014, Pages

  Lafontaine, Eric R ^a   Balder, Rachel ^a   Michel, Frank ^a   Hogan, Robert J ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL GENOME; BACTERIUM ISOLATE; BRONCHUS MUCOSA; BURKHOLDERIA MALLEI; BURKHOLDERIA PSEUDOMALLEI; CELL ADHESION; CONTROLLED STUDY; ESCHERICHIA COLI; GENE DISRUPTION; GENE EXPRESSION; GENE IDENTIFICATION; GENE INACTIVATION; GENE MUTATION; GENE PRODUCT; LUNG ALVEOLUS CELL; MOUSE; NONHUMAN;

ADHESINS, BACTERIAL; ANIMALS; BACTERIAL ADHESION; BACTERIAL OUTER MEMBRANE PROTEINS; BURKHOLDERIA MALLEI; BURKHOLDERIA PSEUDOMALLEI; EPITHELIAL CELLS; ESCHERICHIA COLI; FEMALE; GENE DELETION; GENE EXPRESSION; HUMANS; MEMBRANE TRANSPORT PROTEINS; MICE; MICE, INBRED BALB C; RECOMBINANT PROTEINS;

EID: 84899640326     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-14-92     Document Type: Article

References (86)

1. Neisseria meningitidis NadA is a new invasin which promotes bacterial adhesion to and penetration into human epithelial cells. Capecchi B, Adu-Bobie J, Di Marcello F, Ciucchi L, Masignani V, Taddei A, Rappuoli R, Pizza M, Arico B, Mol Microbiol 2005 55 3 687 698 15660996 (Pubitemid 40203686)
2. Molecular analysis of transport and oligomerization of the Yersinia enterocolitica adhesin YadA. Roggenkamp A, Ackermann N, Jacobi CA, Truelzsch K, Hoffmann H, Heesemann J, J Bacteriol 2003 185 13 3735 3744 10.1128/JB.185.13. 3735-3744.2003 12813066 (Pubitemid 36735923)
3. The UspA2 protein of Moraxella catarrhalis is directly involved in the expression of serum resistance. Attia AS, Lafontaine ER, Latimer JL, Aebi C, Syrogiannopoulos GA, Hansen EJ, Infect Immun 2005 73 4 2400 2410 10.1128/IAI.73.4.2400-2410.2005 15784586 (Pubitemid 40470843)
4. Molecular characterization of a secreted enzyme with phospholipase B activity from Moraxella bovis. Farn JL, Strugnell RA, Hoyne PA, Michalski WP, Tennent JM, J Bacteriol 2001 183 22 6717 6720 10.1128/JB.183.22.6717-6720.2001 11673447 (Pubitemid 33026768)
5. The Moraxella catarrhalis autotransporter McaP is a conserved surface protein that mediates adherence to human epithelial cells through its N-terminal passenger domain. Lipski SL, Akimana C, Timpe JM, Wooten RM, Lafontaine ER, Infect Immun 2007 75 1 314 324 10.1128/IAI.01330-06 17088358 (Pubitemid 46047907)
6. Identification of a Moraxella catarrhalis outer membrane protein exhibiting both adhesin and lipolytic activities. Timpe JM, Holm MM, Vanlerberg SL, Basrur V, Lafontaine ER, Infect Immun 2003 71 8 4341 4350 10.1128/IAI.71.8.4341-4350.2003 12874311 (Pubitemid 36919898)
7. Protease activity, secretion, cell entry, cytotoxicity, and cellular targets of secreted autotransporter toxin of uropathogenic Escherichia coli. Maroncle NM, Sivick KE, Brady R, Stokes FE, Mobley HL, Infect Immun 2006 74 11 6124 6134 10.1128/IAI.01086-06 16954394 (Pubitemid 44657391)
8. Regions important for the adhesin activity of Moraxella catarrhalis Hag. Bullard B, Lipski S, Lafontaine ER, BMC Microbiol 2007 7 65 10.1186/1471-2180-7- 65 17608944 (Pubitemid 47101559)
9. Identification of a Moraxella catarrhalis gene that confers adherence to various human epithelial cell lines in vitro. Lipski SL, Holm MM, Lafontaine ER, FEMS Microbiol Lett 2007 267 2 207 213 10.1111/j.1574-6968.2006.00549.x 17166229 (Pubitemid 46115965)
10. Biological Trojan horse: antigen 43 provides specific bacterial uptake and survival in human neutrophils. Fexby S, Bjarnsholt T, Jensen PO, Roos V, Hoiby N, Givskov M, Klemm P, Infect Immun 2007 75 1 30 34 10.1128/IAI.01117-06 17030570 (Pubitemid 46047875)
11. Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant. Stevens JM, Ulrich RL, Taylor LA, Wood MW, Deshazer D, Stevens MP, Galyov EE, J Bacteriol 2005 187 22 7857 7862 10.1128/JB.187.22.7857-7862.2005 16267310 (Pubitemid 41587723)
12. Structure-function analysis of the self-recognizing Antigen 43 autotransporter protein from Escherichia coli. Klemm P, Hjerrild L, Gjermansen M, Schembri MA, Mol Microbiol 2004 51 1 283 296 14651628 (Pubitemid 38031088)
13. The antigen 43 structure reveals a molecular Velcro-like mechanism of autotransporter-mediated bacterial clumping. Heras B, Totsika M, Peters KM, Paxman JJ, Gee CL, Jarrott RJ, Perugini MA, Whitten AE, Schembri MA, Proc Natl Acad Sci U S A 2014 111 1 457 462 10.1073/pnas.1311592111 24335802
14. UpaG, a new member of the trimeric autotransporter family of adhesins in uropathogenic Escherichia coli. Valle J, Mabbett AN, Ulett GC, Toledo-Arana A, Wecker K, Totsika M, Schembri MA, Ghigo JM, Beloin C, J Bacteriol 2008 190 12 4147 4167 10.1128/JB.00122-08 18424525 (Pubitemid 351812861)
15. Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli: cell aggregation and biofilm formation. Sherlock O, Schembri MA, Reisner A, Klemm P, J Bacteriol 2004 186 23 8058 8065 10.1128/JB.186.23.8058-8065.2004 15547278 (Pubitemid 39552517)
16. Trimeric autotransporters: a distinct subfamily of autotransporter proteins. Cotter SE, Surana NK, 3rd St Geme JW, Trends Microbiol 2005 13 5 199 205 10.1016/j.tim.2005.03.004 15866036 (Pubitemid 40603902)
17. The great escape: structure and function of the autotransporter proteins. Henderson IR, Navarro-Garcia F, Nataro JP, Trends Microbiol 1998 6 9 370 378 10.1016/S0966-842X(98)01318-3 9778731 (Pubitemid 28473198)
18. Secretion of virulence determinants by the general secretory pathway in gram-negative pathogens: an evolving story. Stathopoulos C, Hendrixson DR, Thanassi DG, Hultgren SJ, 3rd St Geme JW, 3rd Curtiss R, Microbes Infect 2000 2 9 1061 1072 10.1016/S1286-4579(00)01260-0 10967286 (Pubitemid 30664220)
19. Type V protein secretion pathway: the autotransporter story. Henderson IR, Navarro-Garcia F, Desvaux M, Fernandez RC, Ala'Aldeen D, Microbiol Mol Biol Rev 2004 68 4 692 744 10.1128/MMBR.68.4.692-744.2004 15590781 (Pubitemid 39628534)
20. Trimeric autotransporter adhesins: variable structure, common function. Linke D, Riess T, Autenrieth IB, Lupas A, Kempf VA, Trends Microbiol 2006 14 6 264 270 10.1016/j.tim.2006.04.005 16678419 (Pubitemid 43825731)
21. Structure and sequence analysis of Yersinia YadA and Moraxella UspAs reveal a novel class of adhesins. Hoiczyk E, Roggenkamp A, Reichenbecher M, Lupas A, Heesemann J, Embo J 2000 19 22 5989 5999 10.1093/emboj/19.22.5989 11080146
22. Intranasal immunization of mice with recombinant Streptococcus gordonii expressing NadA of Neisseria meningitidis induces systemic bactericidal antibodies and local IgA. Ciabattini A, Giomarelli B, Parigi R, Chiavolini D, Pettini E, Arico B, Giuliani MM, Santini L, Medaglini D, Pozzi G, Vaccine 2008 26 33 4244 4250 10.1016/j.vaccine.2008.05.049 18582996
23. Mucosal vaccination against serogroup B meningococci: induction of bactericidal antibodies and cellular immunity following intranasal immunization with NadA of Neisseria meningitidis and mutants of Escherichia coli heat-labile enterotoxin. Bowe F, Lavelle EC, McNeela EA, Hale C, Clare S, Arico B, Giuliani MM, Rae A, Huett A, Rappuoli R, Dougan G, Mills KH, Infect Immun 2004 72 7 4052 4060 10.1128/IAI.72.7.4052-4060.2004 15213150 (Pubitemid 38821920)
24. The C-terminal fragment of the internal 110-kilodalton passenger domain of the Hap protein of nontypeable Haemophilus influenzae is a potential vaccine candidate. Liu DF, Mason KW, Mastri M, Pazirandeh M, Cutter D, Fink DL, 3rd St Geme JW, Zhu D, Green BA, Infect Immun 2004 72 12 6961 6968 10.1128/IAI.72.12. 6961-6968.2004 15557618 (Pubitemid 39552364)
25. Immunization with Haemophilus influenzae Hap adhesin protects against nasopharyngeal colonization in experimental mice. Cutter D, Mason KW, Howell AP, Fink DL, Green BA, 3rd St Geme JW, J Infect Dis 2002 186 8 1115 1121 10.1086/344233 12355362 (Pubitemid 35177114)
26. Vaccination with proteus toxic agglutinin, a hemolysin-independent cytotoxin in vivo, protects against Proteus mirabilis urinary tract infection. Alamuri P, Eaton KA, Himpsl SD, Smith SN, Mobley HL, Infect Immun 2009 77 2 632 641 10.1128/IAI.01050-08 19029299
27. Glanders: New Insights into an old Disease. Waag DM, Deshazer D, Biological Weapons Defense: Infectious Diseases and Counterbioterrorism Totowa, New Jersey: Humana Press Inc, Lindler LE LF, Korch GW, 2004
28. Alibek K, Handelman S, Biohazard: The Chilling True Story of the Largest Covert Biological Weapons Programin the World New York: Random House 1999
29. [Glanders - a potential disease for biological warfare in humans and animals]. Lehavi O, Aizenstien O, Katz LH, Hourvitz A, Harefuah 2002 141 119 Spec No:88-91
30. First shots fired in biological warfare. Wheelis M, Nature 1998 395 6699 213 9751039 (Pubitemid 28447526)
31. Biological Sabotage in World War I. Wheelis M, Biological and Toxin Weapons: Research, Development, and use from the Middle Ages to 1945 Oxford: Oxford University Press, Geissler EMoon JEC, 1999 35 72
32. Strategies toward vaccines against Burkholderia mallei and Burkholderia pseudomallei. Bondi SK, Goldberg JB, Expert Rev Vaccines 2008 7 9 1357 1365 10.1586/14760584.7.9.1357 18980539
33. Molecular insights into Burkholderia pseudomallei and Burkholderia mallei pathogenesis. Galyov EE, Brett PJ, Deshazer D, Annu Rev Microbiol 2010 64 495 517 10.1146/annurev.micro.112408.134030 20528691
34. Equine glanders in Turkey. Arun S, Neubauer H, Gurel A, Ayyildiz G, Kuscu B, Yesildere T, Meyer H, Hermanns W, Vet Rec 1999 144 10 255 258 10.1136/vr.144.10.255 10209817 (Pubitemid 129544471)
35. Human glanders. Neubauer H, Meyer H, Finke EJ, Revue Internationale des Services de Sante des Forces Armees 1997 70 258 265
36. Glanders: off to the races with Burkholderia mallei. Whitlock GC, Estes DM, Torres AG, FEMS Microbiol Lett 2007 277 2 115 122 10.1111/j.1574-6968.2007. 00949.x 18031330 (Pubitemid 350145570)
37. Glanders in a military research microbiologist. Srinivasan A, Kraus CN, DeShazer D, Becker PM, Dick JD, Spacek L, Bartlett JG, Byrne WR, Thomas DL, N Engl J Med 2001 345 4 256 258 10.1056/NEJM200107263450404 11474663 (Pubitemid 32695065)
38. Glanders. Gregory BC, Waag DM, Medical Aspects of Biological Warfare Washington DC: Borden Institute Walter Reed Army Medical Center U.S Army Medical Department Borden Insitute Textbooks of Biological Warfare. 2007 121 146
39. Melioidosis: epidemiology, pathophysiology, and management. Cheng AC, Currie BJ, Clin Microbiol Rev 2005 18 2 383 416 10.1128/CMR.18.2.383-416.2005 15831829 (Pubitemid 40548298)
40. Melioidosis. Wiersinga WJ, Currie BJ, Peacock SJ, N Engl J Med 2012 367 11 1035 1044 10.1056/NEJMra1204699 22970946
41. Melioidosis: insights into the pathogenicity of Burkholderia pseudomallei. Wiersinga WJ, van der Poll T, White NJ, Day NP, Peacock SJ, Nat Rev Microbiol 2006 4 4 272 282 10.1038/nrmicro1385 16541135
42. Endemic melioidosis in tropical northern Australia: a 10-year prospective study and review of the literature. Currie BJ, Fisher DA, Howard DM, Burrow JN, Lo D, Selva-Nayagam S, Anstey NM, Huffam SE, Snelling PL, Marks PJ Stephens DP, Lum GD, Jacups SP, Krause VL, Clin Infect Dis 2000 31 4 981 986 10.1086/318116 11049780 (Pubitemid 32299357)
43. Melioidosis: acute and chronic disease, relapse and re-activation. Currie BJ, Fisher DA, Anstey NM, Jacups SP, Trans R Soc Trop Med Hyg 2000 94 3 301 304 10.1016/S0035-9203(00)90333-X 10975006 (Pubitemid 30406565)
44. The molecular and cellular basis of pathogenesis in melioidosis: how does Burkholderia pseudomallei cause disease? Adler NR, Govan B, Cullinane M, Harper M, Adler B, Boyce JD, FEMS Microbiol Rev 2009 33 6 1079 1099 10.1111/j.1574-6976.2009.00189.x 19732156
45. Melioidosis. Vietri NJ, Deshazer D, Medical Aspects of Biological Warfare Washington DC: Borden Institute Walter Reed Army Medical Center U.S Army Medical Department Borden Insitute Textbooks of Biological Warfare. 2007 147 166
46. Melioidosis as an emerging global problem. Dance DA, Acta Trop 2000 74 2-3 115 119 10674638 (Pubitemid 30069781)
47. Melioidosis, northeastern Brazil. Rolim DB, Vilar DC, Sousa AQ, Miralles IS, de Oliveira DC, Harnett G, O'Reilly L, Howard K, Sampson I, Inglis TJ, Emerg Infect Dis 2005 11 9 1458 1460 10.3201/eid1109.050493 16229782 (Pubitemid 41209147)
48. Workshop on Treatment of and Postexposure Prophylaxis for Burkholderia pseudomallei and B. mallei infection, 2010. Lipsitz R, Garges S, Aurigemma R, Baccam P, Blaney DD, Cheng AC, Currie BJ, Dance DA, Gee JE, Larsen J, Limmathurotsakul D, Morrow MG, Norton R, O'Mare E, Peacock SJ, Pesik N, Rogers LP, Schweizer HP, Steinmetz I, Tan G, Tan P, Wiersinga WJ, Wuthiekanun V, Smith TL, Emerg Infect Dis 2012 18 12 online report
49. Autotransporters and their role in the virulence of Burkholderia pseudomallei and Burkholderia mallei. Lazar Adler NR, Stevens JM, Stevens MP, Galyov EE, Front Microbiol 2011 2 151 21811486
50. Characterization of BcaA, a putative classical autotransporter protein in Burkholderia pseudomallei. Campos CG, Borst L, Cotter PA, Infect Immun 2013 81 4 1121 1128 10.1128/IAI.01453-12 23340315
51. Functional characterization of Burkholderia pseudomallei trimeric autotransporters. Campos CG, Byrd MS, Cotter PA, Infect Immun 2013 81 8 2788 2799 10.1128/IAI.00526-13 23716608
52. The Yersinia adhesin YadA collagen-binding domain structure is a novel left-handed parallel beta-roll. Nummelin H, Merckel MC, Leo JC, Lankinen H, Skurnik M, Goldman A, Embo J 2004 23 4 701 711 10.1038/sj.emboj.7600100 14765110 (Pubitemid 38418738)
53. Hag directly mediates the adherence of Moraxella catarrhalis to human middle ear cells. Bullard B, Lipski SL, Lafontaine ER, Infect Immun 2005 73 8 5127 5136 10.1128/IAI.73.8.5127-5136.2005 16041029 (Pubitemid 41105671)
54. Hag mediates adherence of Moraxella catarrhalis to ciliated human airway cells. Balder R, Krunkosky TM, Nguyen CQ, Feezel L, Lafontaine ER, Infect Immun 2009 77 10 4597 4608 10.1128/IAI.00212-09 19667048
55. Identification of Burkholderia mallei and Burkholderia pseudomallei adhesins for human respiratory epithelial cells. Balder R, Lipski S, Lazarus JJ, Grose W, Wooten RM, Hogan RJ, Woods DE, Lafontaine ER, BMC Microbiol 2010 10 250 10.1186/1471-2180-10-250 20920184
56. Identification of a Predicted Trimeric Autotransporter Adhesin Required for Biofilm Formation of Burkholderia pseudomallei. Lazar Adler NR, Dean RE, Saint RJ, Stevens MP, Prior JL, Atkins TP, Galyov EE, PLoS One 2013 8 11 79461 10.1371/journal.pone.0079461 24223950
57. Characterization of the Moraxella catarrhalis uspA1 and uspA2 genes and their encoded products. Cope LD, Lafontaine ER, Slaughter CA, Hasemann CA Jr, Aebi C, Henderson FW, McCracken GH Jr, Hansen EJ, J Bacteriol 1999 181 13 4026 4034 10383971 (Pubitemid 29295921)
58. Modular arrangement of allelic variants explains the divergence in Moraxella catarrhalis UspA protein function. Brooks MJ, Sedillo JL, Wagner N, Laurence CA, Wang W, Attia AS, Hansen EJ, Gray-Owen SD, Infect Immun 2008 76 11 5330 5340 10.1128/IAI.00573-08 18678659
59. Moraxella catarrhalis binding to host cellular receptors is mediated by sequence-specific determinants not conserved among all UspA1 protein variants. Brooks MJ, Sedillo JL, Wagner N, Wang W, Attia AS, Wong H, Laurence CA, Hansen EJ, Gray-Owen SD, Infect Immun 2008 76 11 5322 5329 10.1128/IAI.00572-08 18678656
60. The UspA1 protein and a second type of UspA2 protein mediate adherence of Moraxella catarrhalis to human epithelial cells in vitro. Lafontaine ER, Cope LD, Aebi C, Latimer JL, McCracken GH Jr, Hansen EJ, J Bacteriol 2000 182 5 1364 1373 10.1128/JB.182.5.1364-1373.2000 10671460 (Pubitemid 30104416)
61. Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei. Moore RA, DeShazer D, Reckseidler S, Weissman A, Woods DE, Antimicrob Agents Chemother 1999 43 3 465 470 10049252 (Pubitemid 29109504)
62. Moraxella catarrhalis strain O35E expresses two filamentous hemagglutinin-like proteins that mediate adherence to human epithelial cells. Balder R, Hassel J, Lipski S, Lafontaine ER, Infect Immun 2007 75 6 2765 2775 10.1128/IAI.00079-07 17371858 (Pubitemid 46870156)
63. Effects of TNF-alpha on expression of ICAM-1 in human airway epithelial cells in vitro. Signaling pathways controlling surface and gene expression. Krunkosky TM, Fischer BM, Martin LD, Jones N, Akley NJ, Adler KB, Am J Respir Cell Mol Biol 2000 22 6 685 692 10.1165/ajrcmb.22.6.3925 10837365 (Pubitemid 30400247)
64. Mycoplasma pneumoniae host-pathogen studies in an air-liquid culture of differentiated human airway epithelial cells. Krunkosky TM, Jordan JL, Chambers E, Krause DC, Microb Pathog 2007 42 2-3 98 103 17261358 (Pubitemid 46283324)
65. Identification of gene products involved in Biofilm production by Moraxella catarrhalis ETSU-9 in vitro. Pearson MM, Hansen EJ, Infect Immun 2007 75 9 4316 4325 10.1128/IAI.01347-06 17562762 (Pubitemid 47378088)
66. A UspA2H-negative variant of Moraxella catarrhalis strain O46E has a deletion in a homopolymeric nucleotide repeat common to uspA2H genes. Wang W, Pearson MM, Attia AS, Blick RJ, Hansen EJ, Infect Immun 2007 75 4 2035 2045 10.1128/IAI.00609-06 17220316 (Pubitemid 46559473)
67. Use of a safe, reproducible and rapid aerosol delivery method to study infection by Burkholderia pseudomallei and Burkholderia mallei in mice. Lafontaine ER, Zimmerman S, Shaffer TL, Michel F, Gao X, Hogan RJ, PLoS One 2013 8 10 76804 10.1371/journal.pone.0076804 24098563
68. Identification of a bacterial factor required for actin-based motility of Burkholderia pseudomallei. Stevens MP, Stevens JM, Jeng RL, Taylor LA, Wood MW, Hawes P, Monaghan P, Welch MD, Galyov EE, Mol Microbiol 2005 56 1 40 53 10.1111/j.1365-2958.2004.04528.x 15773977 (Pubitemid 40461486)
69. The BpeAB-OprB efflux pump of Burkholderia pseudomallei 1026b does not play a role in quorum sensing, virulence factor production, or extrusion of aminoglycosides but is a broad-spectrum drug efflux system. Mima T, Schweizer HP, Antimicrob Agents Chemother 2010 54 8 3113 3120 10.1128/AAC.01803-09 20498323
70. Burkholderia Hep-Hap autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis. Tiyawisutsri R, Holden MT, Tumapa S, Rengpipat S, Clarke SR, Foster SJ, Nierman WC, Day NP, Peacock SJ, BMC Microbiol 2007 7 19 10.1186/1471-2180-7-19 17362501 (Pubitemid 46536651)
71. Persistent gastric colonization with Burkholderia pseudomallei and dissemination from the gastrointestinal tract following mucosal inoculation of mice. Goodyear A, Bielefeldt-Ohmann H, Schweizer H, Dow S, PLoS One 2012 7 5 37324 10.1371/journal.pone.0037324 22624016
72. A non-invasive intratracheal inoculation method for the study of pulmonary melioidosis. Revelli DA, Boylan JA, Gherardini FC, Front Cell Infect Microbiol 2012 2 164 23267442
73. Characterization of a murine model of melioidosis: comparison of different strains of mice. Hoppe I, Brenneke B, Rohde M, Kreft A, Haussler S, Reganzerowski A, Steinmetz I, Infect Immun 1999 67 6 2891 2900 10338496 (Pubitemid 29244705)
74. BALB/c and C57Bl/6 mice infected with virulent Burkholderia pseudomallei provide contrasting animal models for the acute and chronic forms of human melioidosis. Leakey AK, Ulett GC, Hirst RG, Microb Pathog 1998 24 5 269 275 10.1006/mpat.1997.0179 9600859 (Pubitemid 28266810)
75. Structural flexibility in the Burkholderia mallei genome. Nierman WC, DeShazer D, Kim HS, Tettelin H, Nelson KE, Feldblyum T, Ulrich RL, Ronning CM, Brinkac LM, Daugherty SC, Davidsen TD, Deboy RT, Dimitrov G, Dodson RJ, Durkin AS, Gwinn ML, Haft DH, Khouri H, Kolonay JF, Madupu R, Mohammoud Y, Nelson WC, Radune D, Romero CM, Sarria S, Selengut J, Shamblin C, Sullivan SA, White O, Yu Y, et al. Proc Natl Acad Sci U S A 2004 101 39 14246 14251 10.1073/pnas. 0403306101 15377793 (Pubitemid 39305060)
76. A broad host range mobilisation system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Simon R, Priefer U, Puhler A, Bio/Technology 1983 1 784 791 10.1038/nbt1183-784 (Pubitemid 14239484)
77. The Moraxella catarrhalis porin-like outer membrane protein CD is an adhesin for human lung cells. Holm MM, Vanlerberg SL, Foley IM, Sledjeski DD, Lafontaine ER, Infect Immun 2004 72 4 1906 1913 10.1128/IAI.72.4.1906-1913.2004 15039309 (Pubitemid 38419900)
78. Positive selection vectors for allelic exchange. Skorupski K, Taylor RK, Gene 1996 169 1 47 52 10.1016/0378-1119(95)00793-8 8635748 (Pubitemid 26075696)
79. Molecular Cloning: A Laboratory Manual (Third Edition). Sambrook J, Russell DW, Woodbury NY: Cold Spring Harbor Laboratory Press 3 2001
80. Identification of the acid phosphatase (acpA) gene homologues in pathogenic and non-pathogenic Burkholderia spp. facilitates TnphoA mutagenesis. Burtnick M, Bolton A, Brett P, Watanabe D, Woods D, Microbiology 2001 147 Pt 1 111 120 11160805 (Pubitemid 32094314)
81. Phenotypic effect of isogenic uspA1 and uspA2 mutations on Moraxella catarrhalis 035E. Aebi C, Lafontaine ER, Cope LD, Latimer JL, Lumbley SL, McCracken GH Jr, Hansen EJ, Infect Immun 1998 66 7 3113 3119 9632574 (Pubitemid 28303105)
82. Rapid microprocedure for isolating detergent-insoluble outer membrane proteins from Haemophilus species. Carlone GM, Thomas ML, Rumschlag HS, Sottnek FO, J Clin Microbiol 1986 24 3 330 332 3489731 (Pubitemid 16046386)
83. Use of the Chinchilla model to evaluate the vaccinogenic potential of the Moraxella catarrhalis filamentous hemagglutinin-like proteins MhaB1 and MhaB2. Shaffer TL, Balder R, Buskirk SW, Hogan RJ, Lafontaine ER, PLoS One 2013 8 7 67881 10.1371/journal.pone.0067881 23844117
84. Antigenic characterization of the oligosaccharide portion of the lipooligosaccharide of nontypable Haemophilus influenzae. Patrick CC, Kimura A, Jackson MA, Hermanstorfer L, Hood A, McCracken GH Jr, Hansen EJ, Infect Immun 1987 55 12 2902 2911 2445682 (Pubitemid 18008255)
85. Expression of the Moraxella catarrhalis UspA1 protein undergoes phase variation and is regulated at the transcriptional level. Lafontaine ER, Wagner NJ, Hansen EJ, J Bacteriol 2001 183 5 1540 1551 10.1128/JB.183.5.1540-1551.2001 11160084 (Pubitemid 32172296)
86. A simple method for estimating fifty percent end points. Reed LJ, Muench H, Am J Hyg 1938 27 793 497