The UspA1 protein and a second type of UspA2 protein mediate adherence of Moraxella catarrhalis to human epithelial cells in vitro

Journal of Bacteriology

Volumn 182, Issue 5, 2000, Pages 1364-1373

  Lafontaine, Eric R ^a   Cope, Leslie D ^a   Aebi, Christoph ^a,b   Latimer, Jo L ^a   McCracken Jr , George H ^a   Hansen, Eric J ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CELL SURFACE PROTEIN; MUTANT PROTEIN; PROTEIN USPA1; PROTEIN USPA2; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM ADHERENCE; CONTROLLED STUDY; EPITHELIUM CELL; GENE INACTIVATION; GENETIC VARIABILITY; HUMAN; HUMAN CELL; MORAXELLA CATARRHALIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRAIN DIFFERENCE;

AMINO ACID SEQUENCE; BACTERIAL ADHESION; BACTERIAL OUTER MEMBRANE PROTEINS; CLONING, MOLECULAR; DNA, BACTERIAL; EPITHELIAL CELLS; GENE EXPRESSION REGULATION, BACTERIAL; HAEMOPHILUS INFLUENZAE; HUMANS; IMMUNE SERA; MOLECULAR SEQUENCE DATA; MORAXELLA (BRANHAMELLA) CATARRHALIS; MUTATION; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; SPECIES SPECIFICITY;

BACTERIA (MICROORGANISMS); HAEMOPHILUS INFLUENZAE; MORAXELLA CATARRHALIS; POSIBACTERIA;

EID: 0033954069     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.5.1364-1373.2000     Document Type: Article

References (43)

1. Aebi, C., L. D. Cope, J. L. Latimer, S. E. Thomas, C. A. Slaughter, G. H. McCracken, Jr., and E. J. Hansen. 1998. Mapping of a protective epitope of the CopB outer membrane protein of Moraxella catarrhalis. Infect. Immun. 66:540-548.
2. Aebi, C., E. R. Lafontaine, L. D. Cope, J. L. Latimer, S. R. Lumbley, G. H. McCracken, Jr., and E. J. Hansen. 1998. Phenotypic effect of isogenic uspA1 and uspA2 mutations on Moraxella catarrhalis 035E. Infect. Immun. 66:3113-3119.
3. Aebi, C., I. Maciver, J. L. Latimer, L. D. Cope, M. K. Stevens, S. E. Thomas, G. H. McCracken, Jr., and E. J. Hansen. 1997. A protective epitope of Moraxella catarrhalis is encoded by two different genes. Infect. Immun. 65: 4367-4377.
4. Aebi, C., B. Stone, M. Beucher, L. D. Cope, I. Maciver, S. E. Thomas, G. H. McCracken, Jr., P. F. Sparling, and E. J. Hansen. 1996. Expression of the CopB outer membrane protein by Moraxella catarrhalis is regulated by iron and affects iron acquisition from transferrin and lactoferrin. Infect. Immun. 64:2024-2030.
5. Beaulieu, D., M. Ouellette, M. G. Bergeron, and P. H. Roy. 1988. Characterization of a plasmid isolated from Branhamella catarrhalis and detection of plasmid sequences within the genome of a B. catarrhalis strain. Plasmid 20:158-162.
6. Bluestone, C. D., J. S. Stephenson, and L. M. Martin. 1992. Ten-year review of otitis media pathogens. Pediatr. Infect. Dis. J. 11:S7-S11.
7. Catlin, B. W., and L. S. Cunningham. 1964. Genetic transformation of Neisseria catarrhalis hy deoxyribonucleate preparations having different average base compositions. J. Gen. Microbiol. 37:341-352.
8. Chen, D., V. Barniak, K. R. VanDerMeid, and J. C. McMichael. 1999. The levels and bactericidal capacity of antibodies directed against the UspA1 and UspA2 outer membrane proteins of Moraxella (Branhamella) catarrhalis in adults and children. Infect. Immun. 67:1310-1316.
9. Chen, D., J. C. McMichael, K. R. van der Meid, D. Hahn, T. Mininni, J. Cowell, and J. Eldridge. 1996. Evaluation of purified UspA from Moraxella catarrhalis as a vaccine in a murine model after active immunization. Infect. Immun. 64:1900-1905.
10. Cope, L. D., E. R. Lafontaine, C. A. Slaughter, C. A. Hasemann, Jr., C. Aebi, F. W. Henderson, and G. H. McCracken, Jr. 1999. Characterization of the Moraxella catarrhalis uspA1 and uspA2 genes and their encoded products. J. Bacteriol. 181:4026-4034.
11. Cope, L. D., S. R. Lumbley, J. L. Latimer, J. Klesney-Tait, M. K. Stevens, L. S. Johnson, M. Purven, R. S. Munson, Jr., T. Lagergard, J. D. Radolf, and E. J. Hansen. 1997. A diffusible cytotoxin of Haemophilus ducreyi. Proc. Natl. Acad. Sci. USA 94:4056-4061.
12. Du, R. P., Q. J. Wang, Y.-P. Yang, A. B. Schryvers, P. Chong, M. H. Klein, and S. M. Loosmore. 1998. Cloning and expression of the Moraxella catarrhalis lactoferrin receptor genes. Infect. Immun. 66:3656-3665.
13. Fitzgerald, M., R. Mulcahy, S. Murphy, C. Keane, D. Coakley, and T. Scott. 1997. A 200 kDa protein is associated with haemagglutinating isolates of Moraxella (Branhaemella) catarrhalis. FEMS Immunol. Med. Microbiol. 18: 209-216.
14. Fitzgerald, M., R. Mulcahy, S. Murphy, C. Keane, D. Coakley, and T. Scott. 1999. Transmission electron microscopy studies of Moraxella (Branhamella) catarrhalis. FEMS Immunol. Med. Microbiol. 23:57-66.
15. Gu, X.-X., J. Chen, S. J. Barenkamp, J. B. Robbins, C.-M. Tsai, D. J. Lim, and J. Battey. 1998. Synthesis and characterization of lipooligosaccharide-based conjugates as vaccine candidates for Moraxella (Branhamella) catarrhalis. Infect. Immun. 66:1891-1897.
16. Hager, H., A. Verghese, S. Alvarez, and S. L. Berk. 1987. Branhamella catarrhalis respiratory infections. Rev. Infect. Dis. 9:1140-1149.
17. Halter, R., J. Pohlner, and T. F. Meyer. 1989. Mosaic-like organization of IgA protease genes in Neisseria gonorrhoeae generated by horizontal genetic exchange in vivo. EMBO J. 8:2737-3744.
18. Hansen, E. J., F. R. Gonzales, N. R. Chamberlain, M. V. Norgard, E. E. Miller, L. D. Cope, S. E. Pelzel, B. Gaddy, and A. Clausell. 1988. Cloning of the gene encoding the major outer membrane protein of Haemophilus influenzae type b. Infect. Immun. 56:2709-2716.
19. Helminen, M. E., I. Maciver, J. L. Latimer, L. D. Cope, G. H. McCracken, Jr., and E. J. Hansen. 1993. A major outer membrane protein of Moraxella catarrhalis is a target for antibodies that enhance pulmonary clearance of the pathogen in an animal model. Infect. Immun. 61:2003-2010.
20. Helminen, M. E., I. Maciver, J. L. Latimer, J. Klesney-Tait, L. D. Cope, M. M. Paris, G. H. McCracken, Jr., and E. J. Hansen. 1994. A large, antigenically conserved protein on the surface of Moraxella catarrhalis is a target for protective antibodies. J. Infect. Dis. 170:867-872.
21. Helminen, M. E., I. Maciver, J. L. Latimer, S. R. Lumbley, L. D. Cope, G. H. McCracken, Jr., and E. J. Hansen. 1993. A mutation affecting expression of a major outer membrane protein of Moraxella catarrhalis alters serum resistance and survival of this organism in vivo. J. Infect. Dis. 168:1194-1201.
22. Henderson, I. R., F. Navarro-Garcia, and J. P. Nataro. 1998. The great escape: structure and function of the autotransporter proteins. Trends Microbiol. 6:370-378.
23. Hobbs, M. M., A. Seiler, M. Achtman, and J. G. Cannon. 1994. Microevolution within a clonal population of pathogenic bacteria: recombination, gene duplication and horizontal genetic exchange in the opa gene family of Neisseria meningitidis. Mol. Microbiol. 12:171-180.
24. Hsiao, C. B., S. Sethi, and T. F. Murphy. 1995. Outer membrane protein CD of Branhamella catarrhalis: sequence conservation in strains recovered from the human respiratory tract. Microb. Pathog. 19:215-225.
25. Jordan, K. L., S. H. Berk, and S. L. Berk. 1990. A comparison of serum bactericidal activity and phenotypic characteristics of hacteremic, pneumonia-causing strains, and colonizing strains of Branhamella catarrhalis. Am. J. Med. 88(5A):28S-32S.
26. Klingman, K. L., and T. F. Murphy. 1994. Purification and characterization of a high-molecular-weight outer membrane protein of Moraxella (Branhamella) catarrhalis. Infect. Immun. 62:1150-1155.
27. Kyd, J. M., A. W. Cripps, and T. F. Murphy. 1998. Outer-membrane antigen expression by Moraxella (Branhamella) catarrhalis influences pulmonary clearance. J. Med. Microbiol. 47:159-168.
28. Luke, N. R., T. A. Russo, N. Luther, and A. A. Campagnari. 1999. Use of an isogenic mutant constructed in Moraxella catarrhalis to identify a protective epitope of outer membrane B1 defined by monoclonal antibody 11C6. Infect. Immun. 67:681-687.
29. Maiden, M. C. J. 1993. Population genetics of a transformable bacterium: the influence of horizontal genetic exchange on the biology of Neisseria meningitidis. FEMS Microbiol. Lett. 112:243-250.
30. McGraw, E. A., J. Li, R. K. Selander, and T. S. Whittman. 1999. Molecular evolution and mosaic structure of alpha, beta and gamma intimins of pathogenic Escherichia coli. Mol. Biol. Evol. 16:12-22.
31. McMichael, J. C., M. J. Fiske, R. A. Fredenburg, D. N. Chakravarti, K. R. VanDerMeid, V. Barniak, J. Caplan, E. Bortell, S. Baker, R. Arumugham, and D. Chen. 1998. Isolation and characterization of two proteins from Moraxella catarrhalis that bear a common epitope. Infect. Immun. 66:4374-4381.
32. Murphy, T. F. 1996. Branhamella catarrhalis: epidemiology, surface antigenic structure, and immune response. Microbiol. Rev. 60:267.
33. Murphy, T. F., J. M. Kyd, A. John, C. Kirkham, and A. W. Cripps. 1998. Enhancement of pulmonary clearance of Moraxella (Branhamella) catarrhalis following immunization with outer membrane protein CD in a mouse model. J. Infect. Dis. 178:1667-1675.
34. Ohtsubo, H., K. Nyman, W. Doroszkiewicz, and E. Othsubo. 1981. Multiple copies of iso-insertion sequence of IS1 in Shigella dysenteriae chromosome. Nature 292:640-643.
35. Pelton, S. I., and J. O. Klein. 1999. The promise of immunoprophylaxis for prevention of acute otitis media. Pediatr. Infect. Dis. J. 18:926-935.
36. Reddy, M. S., T. F. Murphy, H. S. Faden, and J. M. Bernstein. 1997. Middle ear mucin glycoprotein: purification and interaction with nontypeable Haemophilus influenzae and Moraxella catarrhalis. Otolaryngol. Head Neck Surg. 116:175-180.
37. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
38. Sethi, S., J. M. Surface, and T. F. Murphy. 1997. Antigenic heterogeneity and molecular analysis of CopB of Moraxella (Branhamella) catarrhalis. Infect. Immun. 65:3666-3671.
39. Setlow, J. K., D. C. Brown, M. E. Boling, A. Mattingly, and M. P. Gordon. 1968. Repair of deoxyribonucleic acid in Haemophilus influenzae. J. Bacteriol. 95:546-558.
40. St. Geme, J. W., III, M. L. de la Morena, and S. Falkow. 1994. A Haemophilus influenzae IgA protease-like protein promotes intimate interaction with human epithelial cells. Mol. Microbiol. 14:217-233.
41. Verduin, C. M., M. Jansze, C. Hol, T. E. Mollnes, J. Verhoef, and H. van Dijk. 1994. Differences in complement activation between complement-resistant and complement-sensitive Moraxella (Branhamella) catarrhalis strains occur at the level of membrane attack complex formation. Infect. Immun. 62:589-595.
42. Whitby, P. W., D. J. Morton, and T. L. Stull. 1998. Construction of antibiotic resistance cassettes with multiple paired restriction sites for insertional mutagenesis of Haemophilus influenzae. FEMS Microbiol. Lett. 158:57-60.
43. Yang, Y.-P., L. E. Myers, U. Mcguinness, P. Chong, Y. Kwok, M. H. Klein, and R. E. Harkness. 1997. The major outer membrane protein, CD, extracted from Moraxella (Branhamella) catarrhalis is a potential vaccine antigen that induces bactericidal antibodies. FEMS Immunol. Med. Microbiol. 17:187-199.