Protein production in Yarrowia lipolytica via fusion to the secreted lipase Lip2p

Molecular Biotechnology

Volumn 56, Issue 1, 2014, Pages 79-90

  Hofmeyer, Thomas ^a   Bulani, Siyavuya Ishmael ^b   Grzeschik, Julius ^a   Krah, Simon ^a   Glotzbach, Bernhard ^a   Uth, Christina ^a   Avrutina, Olga ^a   Brecht, Michael ^a   Göringer, Hans Ulrich ^a   Van Zyl, Petrus ^b   Kolmar, Harald ^a  

^a DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

^b DSI CSIR Nanotechnology Innovation Centre   (South Africa)

Author keywords

Cystine rich proteins; Fusion proteins; High level protein expression; Lipase; Yarrowia lipolytica

Indexed keywords

AMINO ACIDS; ANTIBODIES; COVALENT BONDS; ENZYME ACTIVITY; LIPASES; SULFUR COMPOUNDS;

EXTRACELLULAR MEDIUM; FUSION PROTEINS; HEXAHISTIDINE TAG; PROTEIN EXPRESSIONS; PROTEIN PRODUCTION; SHAKE FLASK CULTIVATIONS; STRUCTURAL COMPLEXITY; YARROWIA LIPOLYTICA;

PURIFICATION;

FUNGAL PROTEIN; GALACTOSE OXIDASE; HEXAHISTIDINE; HYBRID PROTEIN; LIP2P PROTEIN; PROTEIN VARIANT; TRIACYLGLYCEROL LIPASE; TRYPSIN INHIBITOR; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING ASSAY; CELL DENSITY; CELL GROWTH; CONTROLLED STUDY; DISULFIDE BOND; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; ESCHERICHIA COLI; FUNGAL CELL; MICROFILTRATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; SUPERNATANT; YARROWIA LIPOLYTICA;

TOBACCO ETCH VIRUS; YARROWIA LIPOLYTICA;

CLONING, MOLECULAR; CYSTEINE; FUNGAL PROTEINS; GENE EXPRESSION; GENETIC VECTORS; LIPASE; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS; YARROWIA;

EID: 84899488850     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-013-9684-2     Document Type: Article

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