Enhanced genetic tools for engineering multigene traits into green algae

PLoS ONE

Volumn 9, Issue 4, 2014, Pages

  Rasala, Beth A ^a   Chao, Syh Shiuan ^a   Pier, Matthew ^a   Barrera, Daniel J ^a   Mayfield, Stephen P ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CHLAMYDOMONAS REINHARDTII; CHLOROPLAST; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; GENETIC ENGINEERING; GENETIC TRAIT; METABOLIC ENGINEERING; MITOCHONDRION; NONHUMAN; PROTEIN TARGETING; BIOTECHNOLOGY; GENETIC ENHANCEMENT; GENETICS; PROCEDURES; TRANSGENE;

BIOTECHNOLOGY; CHLAMYDOMONAS REINHARDTII; GENETIC ENGINEERING; GENETIC ENHANCEMENT; TRANSGENES;

EID: 84899448875     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0094028     Document Type: Article

References (46)

1. Specht E, Miyake-Stoner S, Mayfield S (2010) Micro-algae come of age as a platform for recombinant protein production. Biotechnol Lett 32: 1373-1383. Available: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid= 2941057&tool=pmcentrez&rendertype=abstract. Accessed 9 August 2013.
2. Jones CS, Mayfield SP (2012) Algae biofuels: versatility for the future of bioenergy. Curr Opin Biotechnol 23: 346-351. Available: http://www.ncbi.nlm. nih.gov/pubmed/22104720. Accessed 14 August 2013.
3. Pulz O, Gross W (2004) Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol 65: 635-648. Available: http://www.ncbi.nlm.nih.gov/pubmed/15300417. Accessed 17 August 2013.
4. Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae. J Biosci Bioeng 101: 87-96. Available: http://www.ncbi.nlm.nih.gov/pubmed/16569602. Accessed 8 August 2013.
5. Raja R, Hemaiswarya S, Kumar NA, Sridhar S, Rengasamy R (2008) A perspective on the biotechnological potential of microalgae. Crit Rev Microbiol 34: 77-88. Available: http://www.ncbi.nlm.nih.gov/pubmed/18568862. Accessed 6 August 2013.
6. TL, Purton S, Becker DK, Collet C (2005) Microalgae as bioreactors. Plant Cell Rep 24: 629-641. Available: http://www.ncbi.nlm.nih.gov/pubmed/16136314. Accessed 9 August 2013.
7. Olaizola M (2003) Commercial development of microalgal biotechnology: from the test tube to the marketplace. Biomol Eng 20: 459-466. Available: http://www.ncbi.nlm.nih.gov/pubmed/12919832. Accessed 12 August 2013.
8. Christaki E, Florou-Paneri P, Bonos E (2011) Microalgae: a novel ingredient in nutrition. Int J Food Sci Nutr 62: 794-799. Available: http://www.ncbi.nlm.nih.gov/pubmed/21574818. Accessed 27 August 2013.
9. Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26: 126-131. Available: http://www.ncbi.nlm.nih.gov/pubmed/18221809. Accessed 7 August 2013.
10. Barnes D, Franklin S, Schultz J, Henry R, Brown E, et al. (2005) Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes. Mol Genet Genomics 274: 625-636. Available: http://www.ncbi.nlm.nih.gov/pubmed/16231149. Accessed 5 August 2013.
11. Mayfield SP, Manuell AL, Chen S, Wu J, Tran M, et al. (2007) Chlamydomonas reinhardtii chloroplasts as protein factories. Curr Opin Biotechnol 18: 126-133. Available: http://www.ncbi.nlm.nih.gov/pubmed/17317144. Accessed 8 August 2013.
12. JN, Oyler GA, Wilkinson L, Betenbaugh MJ (2008) A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution. Curr Opin Biotechnol 19: 430-436. Available: http://www.ncbi.nlm.nih.gov/pubmed/ 18725295. Accessed 6 August 2013.
13. Radakovits R, Jinkerson RE, Darzins A, Posewitz MC (2010) Genetic engineering of algae for enhanced biofuel production. Eukaryot Cell 9: 486-501. Available: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid= 2863401&tool=pmcentrez&rendertype=abstract. Accessed 9 August 2013.
14. Georgianna DR, Mayfield SP (2012) Exploiting diversity and synthetic biology for the production of algal biofuels. Nature 488: 329-335. Available: http://www.ncbi.nlm.nih.gov/pubmed/22895338. Accessed 8 August 2013.
15. Wijffels RH, Kruse O, Hellingwerf KJ (2013) Potential of industrial biotechnology with cyanobacteria and eukaryotic microalgae. Curr Opin Biotechnol 24: 405-413. Available: http://www.ncbi.nlm.nih.gov/pubmed/23647970. Accessed 7 August 2013.
16. Gimpel JA, Specht EA, Georgianna DR, Mayfield SP (2013) Advances in microalgae engineering and synthetic biology applications for biofuel production. Curr Opin Chem Biol 17: 489-495. Available: http://dx.doi.org/10. 1016/j.cbpa.2013.03.038. Accessed 13 August 2013.
17. Beer LL, Boyd ES, Peters JW, Posewitz MC (2009) Engineering algae for biohydrogen and biofuel production. Curr Opin Biotechnol 20: 264-271. Available: http://www.ncbi.nlm.nih.gov/pubmed/19560336. Accessed 7 August 2013.
18. Gong Y, Hu H, Gao Y, Xu X, Gao H (2011) Microalgae as platforms for production of recombinant proteins and valuable compounds: progress and prospects. J Ind Microbiol Biotechnol 38: 1879-1890. Available: http://www.ncbi.nlm.nih.gov/pubmed/21882013. Accessed 6 August 2013.
19. Hannon M, Gimpel J, Tran M, Rasala B, Mayfield S (2010) Biofuels from algae: challenges and potential. Biofuels 1: 763-784. Available: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3152439&tool= pmcentrez&rendertype=abstract.
20. Cerutti H, Johnson AM, Gillham NW, Boynton JE (1997) Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas. Plant Cell 9: 925-945. Available: http://www.plantcell.org/content/9/6/925.abstract. Accessed 27 August 2013.
21. Fuhrmann M, Oertel W, Hegemann P (1999) A synthetic gene coding for the green fluorescent protein (GFP) is a versatile reporter in Chlamydomonas reinhardtii+. Plant J 19: 353-361. Available: http://doi.wiley.com/10.1046/j. 1365-313X.1999.00526.x. Accessed 27 August 2013.
22. Neupert J, Karcher D, Bock R (2009) Generation of Chlamydomonas strains that efficiently express nuclear transgenes. Plant J 57: 1140-1150. Available: http://www.ncbi.nlm.nih.gov/pubmed/19036032. Accessed 21 August 2013.
23. Rasala BA, Lee PA, Shen Z, Briggs SP, Mendez M, et al. (2012) Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. PLoS One 7: e43349. Available: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid= 3427385&tool=pmcentrez&rendertype=abstract. Accessed 30 July 2013.
24. Rasala BA, Barrera DJ, Ng J, Plucinak TM, Rosenberg JN, et al. (2013) Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J 74: 545-556. Available: http://www.ncbi.nlm. nih.gov/pubmed/23521393. Accessed 5 August 2013.
25. Donnelly ML, Luke G, Mehrotra A, Li X, Hughes LE, et al. (2001) Analysis of the aphthovirus 2A/2B polyprotein "cleavage" mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal "skip". J Gen Virol 82: 1013-1025. Available: http://www.ncbi.nlm.nih.gov/pubmed/11297676. Accessed 3 March 2014.
26. De Felipe P (2004) Skipping the co-expression problem: the new 2A "CHYSEL" technology. Genet Vaccines Ther 2: 13. Available: http://www.gvt-journal.com/content/2/1/13. Accessed 16 August 2013.
27. Kalderon D, Roberts BL, Richardson WD, Smith AE (1984) A short amino acid sequence able to specify nuclear location. Cell 39: 499-509. Available: http://dx.doi.org/10.1016/0092-8674(84)90457-4. Accessed 27 August 2013.
28. Morris GJ, Coulson GE, Leeson EA (1985) Changes in the shape of mitochondria following osmotic stress to the unicellular green alga Chlamydomonas reinhardii. J Cell Sci 76: 145-153. Available: http://www.ncbi.nlm.nih.gov/pubmed/3905835. Accessed 27 August 2013.
29. Ehara T, Osafune T, Hase E (1995) Behavior of mitochondria in synchronized cells of Chlamydomonas reinhardtii (Chlorophyta). J Cell Sci 108 (Pt 2: 499-507. Available: http://www.ncbi.nlm.nih.gov/pubmed/7768996. Accessed 27 August 2013.
30. Hiramatsu T, Nakamura S, Misumi O, Kuroiwa T (2006) Morphological Changes In Mitochondrial And Chloroplast Nucleoids And Mitochondria During The Chlamydomonas Reinhardtii (Chlorophyceae) Cell Cycle. J Phycol 42: 1048-1058. Available: http://doi.wiley.com/10.1111/j.1529-8817.2006.00259.x. Accessed 27 August 2013.
31. Harris EH, Stern DB, Witman GB (2009) The Chlamydomonas Sourcebook. Second Edi. Elsevier. Available: http://dx.doi.org/10.1016/B978-0-12-370873-1. 00002-2. Accessed 28 August 2013.
32. Fischer N, Rochaix JD (2001) The flanking regions of PsaD drive efficient gene expression in the nucleus of the green alga Chlamydomonas reinhardtii. Mol Genet Genomics 265: 888-894. Available: http://www.ncbi.nlm.nih.gov/pubmed/ 11523806. Accessed 27 August 2013.
33. Voeltz GK, Rolls MM, Rapoport TA (2002) Structural organization of the endoplasmic reticulum. EMBO Rep 3: 944-950. Available: http://www.pubmedcentral. nih.gov/articlerender.fcgi?artid=1307613&tool=pmcentrez&rendertype= abstract. Accessed 27 August 2013.
34. Gomord V, Denmat L-A, Fitchette-Laine A-C, Satiat-Jeunemaitre B, Hawes C, et al. (1997) The C-terminal HDEL sequence is sufficient for retention of secretory proteins in the endoplasmic reticulum (ER) but promotes vacuolar targeting of proteins that escape the ER. Plant J 11: 313-325. Available: http://doi.wiley.com/10.1046/j.1365-313X.1997.11020313.x. Accessed 27 August 2013.
35. De Hostos EL, Togasaki RK, Grossman A (1988) Purification and biosynthesis of a derepressible periplasmic arylsulfatase from Chlamydomonas reinhardtii. J Cell Biol 106: 29-37. Available: http://www.pubmedcentral.nih. gov/articlerender.fcgi?artid=2114941&tool=pmcentrez&rendertype=abstract. Accessed 28 August 2013.
36. Schroda M (2004) The Chlamydomonas genome reveals its secrets: chaperone genes and the potential roles of their gene products in the chloroplast. Photosynth Res 82: 221-240. Available: http://www.ncbi.nlm.nih.gov/pubmed/ 16143837. Accessed 28 August 2013.
37. Kim JH, Lee S-R, Li L-H, Park H-J, Park J-H, et al. (2011) High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. PLoS One 6: e18556. Available: http://dx.plos.org/10. 1371/journal.pone.0018556. Accessed 12 August 2013.
38. Fang J, Qian J-J, Yi S, Harding TC, Tu GH, et al. (2005) Stable antibody expression at therapeutic levels using the 2A peptide. Nat Biotechnol 23: 584-590. Available: http://www.ncbi.nlm.nih.gov/pubmed/15834403. Accessed 3 February 2014.
39. Szymczak AL, Workman CJ, Wang Y, Vignali KM, Dilioglou S, et al. (2004) Correction of multi-gene deficiency in vivo using a single "self- cleaving" 2A peptide-based retroviral vector. Nat Biotechnol 22: 589-594. Available: http://www.ncbi.nlm.nih.gov/pubmed/15064769. Accessed 20 January 2014.
40. Keasling JD (2012) Synthetic biology and the development of tools for metabolic engineering. Metab Eng 14: 189-195. Available: http://www. sciencedirect.com/science/article/pii/S1096717612000055. Accessed 20 January 2014.
41. Halpin C (2005) Gene stacking in transgenic plants - the challenge for 21st century plant biotechnology. Plant Biotechnol J 3: 141-155. Available: http://www.ncbi.nlm.nih.gov/pubmed/17173615. Accessed 23 January 2014.
42. Ha S-H, Liang YS, Jung H, Ahn M-J, Suh S-C, et al. (2010) Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm. Plant Biotechnol J 8: 928-938. Available: http://www.ncbi.nlm.nih.gov/pubmed/20649940. Accessed 4 February 2014.
43. Zhang F, Rodriguez S, Keasling JD (2011) Metabolic engineering of microbial pathways for advanced biofuels production. Curr Opin Biotechnol 22: 775-783. Available: http://www.sciencedirect.com/science/article/pii/ S0958166911000875. Accessed 20 January 2014.
44. Heinig U, Gutensohn M, Dudareva N, Aharoni A (2013) The challenges of cellular compartmentalization in plant metabolic engineering. Curr Opin Biotechnol 24: 239-246. Available: http://www.ncbi.nlm.nih.gov/pubmed/23246154. Accessed 15 August 2013.
45. Sweetlove LJ, Fernie AR (2013) The spatial organization of metabolism within the plant cell. Annu Rev Plant Biol 64: 723-746. Available: http://www.ncbi.nlm.nih.gov/pubmed/23330793. Accessed 13 August 2013.
46. Dumas P, Bergdoll M, Cagnon C, Masson JM (1994) Crystal structure and site-directed mutagenesis of a bleomycin resistance protein and their significance for drug sequestering. EMBO J 13: 2483-2492. Available: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=395119&tool= pmcentrez&rendertype=abstract. Accessed 4 February 2014.