An innovative LC-MS/MS-based method for determining CYP 17 and CYP 19 activity in the adipose tissue of pre- and postmenopausal and ovariectomized women using 13C-labeled steroid substrates

Journal of Clinical Endocrinology and Metabolism

Volumn 99, Issue 4, 2014, Pages 1339-1347

  Kinoshita, Toshihiko ^a   Honma, Seijiro ^b   Shibata, Yasuhiro ^c   Yamashita, Kouwa ^a,d   Watanabe, Yoko ^b   Maekubo, Hitoe ^b   Okuyama, Mitsunobu ^b   Takashima, Akiko ^a   Takeshita, Naoki ^a  

^a TOHO UNIVERSITY SAKURA MEDICAL CENTER   (Japan)

^b Grelan Pharmaceutical Co Ltd   (Japan)

^c GUNMA UNIVERSITY   (Japan)

^d TOHOKU PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADIPOSE TISSUE; ADULT; AGED; AROMATASE; CARBON ISOTOPES; CHROMATOGRAPHY, LIQUID; FEMALE; HUMANS; MIDDLE AGED; OVARIECTOMY; POSTMENOPAUSE; PREMENOPAUSE; STEROID 17-ALPHA-HYDROXYLASE; TANDEM MASS SPECTROMETRY;

EID: 84898481269     PISSN: 0021972X     EISSN: 19457197     Source Type: Journal    
DOI: 10.1210/jc.2013-3715     Document Type: Article

References (33)

1. Key TJ. Endogenous oestrogens and breast cancer risk in premenopausal and postmenopausal women. Steroids. 2011;76:812-815.
2. Lepine J, Audet-Walsh E, Gregoire J, et al. Circulating estrogens in endometrial cancer cases and their relationship with tissular expression of key estrogen biosynthesis and metabolic pathways. J Clin Endocrinol Metab. 2010;95:2689-2698.
3. Huhtinen K, Desai R, Stahle M, et al. Endometrial and endometri-otic concentrations of estrone and estradiol are determined by local metabolism rather than circulating levels. J Clin Endocrinol Metab. 2012;97:4228-4235.
4. Lonning PE, Haynes BP, Straume AH, et al. Recent data on intra-tumor estrogens in breast cancer. Steroids. 2011;76:786-791.
5. Bulun SE, Simpson ER. Breast cancer and expression of aromatase in Brest adipose tissue. Trends Endocrinol Metab. 1994;5:113-120.
6. Simpson ER, Merrill JC, Hollub AJ, Graham-Lorence S, Mendelson CR. Regulation of estrogen biosynthesis by human adipose cells. Endocrine Rev. 1989;10:136-148.
7. Simpson ER. Role of aromatase in sex steroid action. J Mol Endocrinol. 2000;25:149-156.
8. Deslypere JP, Verdonck L, Vermeulen A. Fat tissue: a steroid reservoir and site of steroid metabolism. J Clin Endocrinol Metab. 1985; 61:564-570.
9. Valle LD, Toffolo V, Nardi A, et al. Tissue-specific transcriptional initiation and activity of steroid sulfatase complementing dehydro- epiandrosterone sulfate uptake and intracrine steroid activations in human adipose tissue. J Endocrinol. 2006;190:129-139.
10. Kershaw EE, Flier JS. Adipose tissue as an endocrine organ. J Clin Endocrinol Metab. 2004;89:2548-2556.
11. Belanger C, Luu-The VL, Dupont P, Tchernof A. Adipose tissue intracrinology: potential importance of local androgen/estrogen metabolism in the regulation of adiposity. Hormone Metab Res. 2002; 34:737-745.
12. Blouin K, Nadeau M, Mailloux J, et al. Pathways of adipose tissue androgen metabolism in women: depot differences and modulation by adipogenesis. Am J Physiol Endocrinol Metab. 2009;296:E244-E255.
13. Montes-Nieo R, Insenser M, Martinez-Garcia MA, Escobar-Mor-reale HF. A nontargeted proteomic study of the influence of andro-gen excess on human visceral and subcutaneous adipose tissue pro-teomes. J Clin Endocrinol Metab. 2013;98:E576-E585.
14. Mackenzie SM, Hudat SS, Sattar N, Fraser R, Connell JMC, Davies E. Depot-specific steroidogenic gene transcription in human adipose tissue. Clin Endocrinol (Oxf). 2008;69:848-854.
15. Blair IA. Analysis of estrogens in serum and plasma from postmeno-pausal women: past present and future. Steroids. 2010;75:297-306.
16. Bailey EJ, Barton DHR, Elks J, Templeton JF. Compounds related to the steroid hormones. Part IX. Oxygenation of steroid ketones in strongly basic medium: a new method of preparation of 17a-hy-droxypregnan-20-ones. J Chem Soc. 1962;1578-1591.
17. Kido T, Tung DV, Manh HD, et al. High cortisol and cortisone levels are associated with breast milk dioxin concentrations in Vietnamese women. Eur J Endocrinol. 2014;170:131-139.
18. Arai S, Miyashiro Y, Shibata Y, et al. New quantification method for estradiol in the prostatic tissues of benign prostatic hyperplasia using liquid chromatography-tandem mass spectrometry. Steroid. 2010; 75:13-19.
19. Higashi T. Salivary hormone measurement using LC/MS/MS: specific and patient-friendly tool for assessment of endocrine function. BiolPharm Bull. 2012;35:1401-1408.
20. Badeau M, Vihma V, Mikkola TS, Tiitinen A, Tikkanen MJ. Es-tradiol fatty acid esters in adipose tissue and serum of pregnant and pre-and postmenopausal women. J Clin Endocrinol Metab. 2007; 92:4327-4331.
21. Yamatani H, Takahashi K, Yoshida T, Takata K, Kurachi H. Association of estrogen with glucocorticoid levels in visceral fat in postmenopausal women. Menopause. 2013;20(4):437-442.
22. Simpson ER. Sources of estrogen and their importance. J Steroid Biochem Mol Biol. 2003;86:225-230.
23. Meseguer A, Puche C, Cabero A. Sex steroid biosynthesis in white adipose tissue. Horm Metab Res. 2002;34:731-736.
24. Szymczak J, Milewicz A, Thijssen JHH, Blankenstein MA, Daro-szewski J. Concentration of sex steroids in adipose tissue after menopause. Steroids. 1998;63:319-321.
25. Blouin K, Richard C, Belanger C, et al. Local androgen inactivation in abdominal visceral adipose tissue. JClin Endocrinol Metab. 2003; 88:5944-5950.
26. Feher T, Bodrogi L. A comparative study of steroid concentrations in human adipose tissue and the peripheral circulation. Clin Chim Acta. 1982;126:135-141.
27. Bulun SE, Sebastian S, Takayama K, Suzuki T, Sasano H, Shozu M. The human CYP19 (aromatase P450) gene: update on physiologic roles and genomic organization of promoters. J Steroid Biochem Mol Biol. 2003;86:219-224.
28. Zhao Y, Agarwal VR, Mendelson CR, Simpson ER. Transcriptional regulation of CYP19 gene (aromatase) expression in adipose stro-mal cells in primary culture. JSteroid Biochem Mol Biol. 1997;61: 203-210.
29. Zhao H, Pearson EK, Brooks DC, et al. A humanized pattern of aromatase expression is associated with mammary hyperplasia in mice. Endocrinology. 2012;153:2701-2713.
30. Yamaoka M, Hara T, Hitaka T, etal. Orteronel (TAK-700),anovel non-steroidal 17,20-lyase inhibitor: effect on steroid synthesis in human and monkey adrenal cells and serum steroid levels in cyno-molgus monkeys. JSteroid Biochem Mol Biol. 2012;129:115-128.
31. Idkowiak J, Randell T, Dhir V, et al. A missense mutation in the human cytochrome b5 gene causes 46, xy disorder of sex development due to true isolated 17,20 lyase deficiency. JClin Endocrinol Metab. 2012;97:E465-E475.
32. Puche C, Jose M, Cabero A, Meseguer A. Expression and enzymatic activity of the P450c17 gene in human adipose tissue. Eur JEndo-crinol. 2002;146:223-229.
33. Sharp L, Cardy AH, Cotton SC, Little J. CYP17 gene polymorphisms: prevalence and associations with hormone levels and related factors. Am J Epidemiol. 2004;160:729-740.