Cationic nanostructured polymers for siRNA Delivery in murine calvarial pre-osteoblasts

Journal of Biomedical Nanotechnology

Volumn 10, Issue 6, 2014, Pages 1130-1136

  Hsu, Eric W ^a   Liu, Shiguang ^a   Shrivats, Arun R ^a   Watt, April C S ^a   McBride, Sean ^a   Averick, Saadyah E ^b   Cho, Hong Y ^b   Matyjaszewski, Krzysztof ^b   Hollinger, Jeffrey O ^a  

^a CARNEGIE MELLON UNIVERSITY   (United States)

^b CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

ATRP; Gapdh knockdown; Nanogel; Nanostructured polymer; siRNA delivery; Star polymer

Indexed keywords

ATOM TRANSFER RADICAL POLYMERIZATION; GENE EXPRESSION; LIPOSOMES; NANOSTRUCTURED MATERIALS; POLYETHYLENE GLYCOLS; PROTEINS; RNA;

GAPDH KNOCKDOWN; NANOGELS; NANOSTRUCTURED POLYMERS; SIRNA DELIVERY; STAR POLYMERS;

POLYMERS;

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; LIPOPLEX; MACROGOL; MESSENGER RNA; NANOMATERIAL; NANOSTRUCTURED POLYMER; POLYMER; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ATOM TRANSFER RADICAL POLYMERIZATION; BIOCOMPATIBILITY; CALVARIA; CELL DIFFERENTIATION; CELL LINE; CELL VIABILITY; COMPLEX FORMATION; CONTROLLED STUDY; EMBRYO; GENE SILENCING; IN VITRO STUDY; INFLAMMATION; MINERALIZATION; MOUSE; NONHUMAN; OSTEOBLAST; PROTEIN EXPRESSION; PROTEIN TARGETING; SYNTHESIS;

ANIMALS; CATIONS; CELLS, CULTURED; CRYSTALLIZATION; LIPIDS; MATERIALS TESTING; MICE; MICE, INBRED C57BL; NANOCAPSULES; OSTEOBLASTS; PARTICLE SIZE; POLYMERS; RNA, SMALL INTERFERING; SKULL; SURFACE PROPERTIES; TRANSFECTION;

EID: 84898400514     PISSN: 15507033     EISSN: 15507041     Source Type: Journal    
DOI: 10.1166/jbn.2014.1823     Document Type: Article

References (48)

1. J. C. Burnett, J. J. Rossi, and K. Tiemann, Current progress of siRNA/shRNA therapeutics in clinical trials. Biotechnol. J. 6, 1130 (2011).
2. N. M. Snead and J. J. Rossi, RNA interference trigger variants: Getting the most out of RNA for RNA interference-based therapeutics. Nucleic Acid Ther. 22, 139 (2012).
3. A. A. Seyhan, RNAi: A potential new class of therapeutic for human genetic disease. Hum. Genet. 130, 583 (2011).
4. L. Timmons, H. Tabara, C. C Mello, and A. Z. Fire, Inducible systemic RNA silencing in Caenorhabditis elegans. Mol. Bio. Cell 14, 2972 (2003).
5. J. W. Gaynor, B. J. Campbell, and R. Cosstick, RNA interference: A chemist's perspective. Chem. Soc. Rev. 39, 4169 (2010).
6. J. Zabner, A. J. Fasbender, T. Moninger, K. A. Poellinger, and M. J. Welsh, Cellular and molecular barriers to gene transfer by a cationic lipid. J. Biol. Chem. 270, 18997 (1995).
7. R. Mulligan, The basic science of gene therapy. Science 260, 926 (1993).
8. C. E. Thomas, A. Ehrhardt, and M. A. Kay, Progress and problems with the use of viral vectors for gene therapy. Nat. Rev. Genet. 4, 346 (2003).
9. Z. Rehman, I. S. Zuhorn, and D. Hoekstra, How cationic lipids transfer nucleic acids into cells and across cellular membranes: Recent advances. J. Control. Rel. 166, 46 (2013).
10. M. T. Calejo, A. M. Cardoso, A. L. Kjøniksen, K. Zhu, C. M. Morais, S. A. Sande, A. L. Cardoso, M. C. Lima, A. Jurado, and B. Nyström, Temperature-responsive cationic block copolymers as nanocarriers for gene delivery. Int. J. Pharm. 448, 105 (2013).
11. R. B. Shmueli, N. S. Bhise, and J. J. Green, Evaluation of polymeric gene delivery nanoparticles by nanoparticle tracking analysis and high-throughput flow cytometry. J. Vis. Exp. e50176 (2013).
12. M. Mahato, P. Kumar, and A. K. Sharma, Amphiphilic polyethylenimine polymers mediate efficient delivery of DNA and siRNA in mammalian cells. Mol. BioSyst. 9, 780 (2013).
13. C. H. Jones, C. K. Chen, M. Jiang, L. Fang, C. Cheng, and B. A. Pfeifer, Synthesis of cationic polylactides with tunable charge densities as nanocarriers for effective gene delivery. Mol. Pharm. 10, 1138 (2013).
14. Z. Ziraksaz, A. Nomani, M. Soleimani, B. Bakhshandeh, E. Arefian, I. Haririan, and M. Tabbakhian, Evaluation of cationic dendrimer and lipid as transfection reagents of short RNAs for stem cell modification. Int. J. Pharm. 448, 231 (2013).
15. H. Zeng, H. C. Little, T. N. Tiambeng, G. A. Williams, and Z. Guan, Multifunctional dendronized peptide polymer platform for safe and effective siRNA delivery. J. Am. Chem. Soc. (2013). Doi:10.1021/ja400986u.
16. S. Biswas, P. P. Deshpande, G. Navarro, N. S. Dodwadkar, and V. P. Torchilin, Lipid modified triblock PAMAM-based nanocarriers for siRNA drug co-delivery. Biomaterials 34, 1289 (2013).
17. I. B. Neagoe, C. Braicu, C. Matea, C. Bele, G. Florin, K. Gabriel, C. Veronica, and A. Irimie, Efficient siRNA delivery system using carboxilated single-wall carbon nanotubes in cancer treatment. J. Biomed. Nanotechnol. 8, 567 (2012).
18. A. D. Tagalakis, L. Saraiva, D. McCarthy, K. T. Gustafsson, and S. L. Hart, Comparison of nanocomplexes with branched and linear peptides for siRNA delivery. Biomacromolecules 14, 761 (2013).
19. K. M. Choi, G. L. Park, K. Y. Hwang, J. W. Lee, and H. J. Ahn, Efficient siRNA delivery into tumor cells by p19-YSA fusion protein. Mol. Pharm. 10, 763 (2013).
20. M. D. Pungente, E. Jubeli, C. L. Øpstad, M. Al-Kawaz, N. Barakat, T. Ibrahim, N. Abdul Khalique, L. Raju, R. Jones, P. L. Leopold, H. R. Sliwka, and V. Partali, Synthesis and preliminary investigations of the siRNA delivery potential of novel, single-chain rigid cationic carotenoid lipids. Molecules 17, 3484 (2012).
21. J. S. Zhang, F. Liu, and L. Huang, Implications of pharmacokinetic behavior of lipoplex for its inflammatory toxicity. Adv. Drug Deliv. Rev. 57, 689 (2005).
22. C. R. Dass, T. L. Walker, and M. A. Burton, Liposomes containing cationic dimethyl dioctadecyl ammonium bromide: Formulation, quality control, and lipofection efficiency. Drug Deliv. 9, 11 (2002).
23. K. Hong, W. Zheng, A. Baker, and D. Papahadjopoulos, Stabilization of cationic liposome-plasmid DNA complexes by polyamines and poly(ethylene glycol)-phospholipid conjugates for efficient in vivo gene delivery. FEBS Lett. 400, 233 (1997).
24. T. Segura and L. D. Shea, Materials for non-viral gene delivery. Ann. Rev. Mater. Res. 31, 25 (2001).
25. R. I. Mahato, Water insoluble and soluble lipids for gene delivery. Adv. Drug Deliv. Rev. 57, 699 (2005).
26. G. Chen, J. Kumar, A. Gregory, and M. H. Stenzel, Efficient synthesis of dendrimers via a thiol-yne and esterification process and their potential application in the delivery of platinum anti-cancer drugs. Chem. Commun. (Camb.) 7, 6291 (2009).
27. C. Boyer, J. Teo, P. Phillips, R. B. Erlich, S. Sagnella, G. Sharbeen, T. Dwarte, H. T. Duong, D. Goldstein, T. P. Davis, M. Kavallaris, and J. McCarroll, Effective delivery of siRNA into cancer cells and tumors using well-defined biodegradable cationic star polymers. Mol. Pharm. 10, 2435 (2013).
28. J. Liu, H. Duong, M. R. Whittaker, T. P. Davis, and C. Boyer, Synthesis of functional core, star polymers via RAFT polymerization for drug delivery applications. Macromol. Rapid Commun. 33, 760 (2012).
29. M. H. Smith and L. A. Lyon, Multifunctional nanogels for siRNA delivery. Acc. Chem. Res. 45, 985 (2012).
30. K. Matyjaszewski and J. Xia, Atom transfer radical polymerization. Chem. Rev. 101, 2921 (2001).
31. K. Matyjaszewski, Atom transfer radical polymerization (ATRP): Current status and future perspectives. Macromolecules 45, 4015 (2012).
32. S. A. Bencherif, H. Gao, A. Srinivasan, D. J. Siegwart, J. O. Hollinger, N. R. Washburn, and K. Matyjaszewski, Cell-adhesive star polymers prepared by ATRP. Biomacromolecules 10, 1795 (2009).
33. H. Y. Cho, A. Srinivasan, J. Hong, E. Hsu, S. Liu, A. Shrivats, D. Kwak, A. K. Bohaty, H. J. Paik, J. O. Hollinger, and K. Matyjaszewski, Synthesis of biocompatible PEG-based star polymers with cationic and degradable core for siRNA delivery. Biomacromolecules 12, 3478 (2011).
34. D. J. Siegwart, A. Srinivasan, S. A. Bencherif, A. Karunanidhi, J. K. Oh, S. Vaidya, R. Jin, J. O. Hollinger, and K. Matyjaszewski, Cellular uptake of functional nanogels prepared by inverse miniemulsion ATRP with encapsulated proteins, carbohydrates, and gold nanoparticles. Biomacromolecules 10, 2300 (2009).
35. H. Y. Cho, S. E. Averick, E. Paredes, K. Wegner, A. Averick, S. Jurga, S. R. Das, and K. Matyjaszewski, Star polymers with a cationic core prepared by ATRP for cellular nucleic acids delivery. Biomacromolecules 14, 1262 (2013).
36. S. E. Averick, E. Paredes, A. Irastorza, A. R. Shrivats, A. Srinivasan, D. J. Siegwart, A. J. Magenau, H. Y. Cho, E. Hsu, A. A. Averick, J. Kim, S. Liu, J. O. Hollinger, S. R. Das, and K. Matyjaszewski, Preparation of cationic nanogels for nucleic acid delivery. Biomacromolecules 13, 3445 (2012).
37. E. M. Czekanska, M. J. Stoddart, R. G. Richards, and J. S. Hayes, In search of an osteoblast cell model for in vitro research. Eur. Cell Mater. 24, 1 (2012).
38. H. Sudo, H. A. Kodama, Y. Amagai, S. Yamamoto, and S. Kasai, In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J. Cell Biol. 96, 191 (1983).
39. A. Billiau and P. Matthys, Modes of action of Freund's adjuvants in experimental models of autoimmune diseases. J. Leukoc. Biol. 70, 849 (2001).
40. S. Kawakami, Y. Ito, P. Charoensit, F. Yamashita, and M. Hashida, Evaluation of proinflammatory cytokine production induced by linear and branched polyethylenimine/plasmid DNA complexes in mice. J. Pharmacol. Exp. Ther. 317, 1382 (2006).
41. L. Parhamifar, A. K. Larsen, A. C. Hunter, T. L. Andresen, and S. M. Moghimi, Polycation cytotoxicity: A delicate matter for nucleic acid therapy - Focus on polyethylenimine. Soft. Matter 6, 4001 (2010).
42. S. M. Moghimi, P. Symonds, J. C. Murray, A. C. Hunter, G. Debska, and A. Szewczyk, A two-stage poly(ethylenimine)-mediated cytotoxicity: Implications for gene transfer/therapy. Mol. Ther. 11, 990 (2005).
43. Y. Duan, X. Guan, J. Ge, D. Quan, Y. Zhuo, H. Ye, and T. Shao, Cationic nano-copolymers mediated IKKβ targeting siRNA inhibit the proliferation of human Tenon's capsule fibroblasts in vitro. Mol. Vis. 2616 (2008).
44. Y. Zhang, M. Zheng, T. Kissel, and S. Agarwal, Design and biophysical characterization of bioresponsive degradable poly(dimethylaminoethyl methacrylate) based polymers for in vitro DNA transfection. Biomacromolecules 13, 313 (2012).
45. M. J. Bruining, H. G. Blaauwgeers, R. Kuijer, E. Pels, R. M. Nuijts, and L. H. Koole, Biodegradable three-dimensional networks of poly(dimethylamino ethyl methacrylate). Synthesis, characterization and in vitro studies of structural degradation and cytotoxicity. Biomaterials 21, 595 (2000).
46. B. Newland, H. Tai, Y. Zheng, D. Velasco, A. Di Luca, S. M. Howdle, C. Alexander, W. Wang, and A. Pandit, A highly effective gene delivery vector-hyperbranched poly(2-(dimethylamino)ethyl methacrylate) from in situ deactivation enhanced ATRP. Chem. Commun. (Camb.) 46, 4698 (2010).
47. H. M. Aliabadi, B. Landry, R. K. Bahadur, A. Neamnark, O. Suwantong, and H. Uludaǧ, Impact of lipid substitution on assembly and delivery of siRNA by cationic polymers. Macromol. Biosci. 11, 662 (2011).
48. J. Suhorutsenko, N. Oskolkov, P. Arukuusk, K. Kurrikoff, E. Eriste, D. M. Copolovici, and U. Langel, Cell-penetrating peptides, pepfects, show no evidence of toxicity and immunogenicity in vitro and in vivo. Bioconjug. Chem. 22, 2255 (2011).