Improved nonreductive O-glycan release by hydrazinolysis with ethylenediaminetetraacetic acid addition

Analytical Biochemistry

Volumn 453, Issue 1, 2014, Pages 29-37

  Kozak, Radoslaw P ^a   Royle, Louise ^a   Gardner, Richard A ^a   Bondt, Albert ^b,c   Fernandes, Daryl L ^a   Wuhrer, Manfred ^c,d  

^a Culham Science Centre   (United Kingdom)

^b ERASMUS MEDICAL CENTER   (Netherlands)

^c LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^d VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

Glycan release; HILIC HPLC; LC ion trap MS MS; O linked glycans; Peeling

Indexed keywords

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDRAZINE; MAMMALS; PEELING; POLYSACCHARIDES;

FLUORESCENT LABELING; GLYCAN RELEASE; GLYCANS; HILIC-HPLC; IMMUNOGLOBULIN A (IGA); IMPROVED TECHNIQUES; ION TRAPS; SUBMAXILLARY GLANDS;

ETHYLENEDIAMINETETRAACETIC ACID;

BOVINE FETUIN; BOVINE SUBMAXILLARY GLAND MUCIN; EDETIC ACID; FETUIN; FLUORESCENT DYE; GLYCAN; HYDRAZINE; IMMUNOGLOBULIN A; MUCIN; SODIUM CHLORIDE; UNCLASSIFIED DRUG; HYDRAZINE DERIVATIVE; POLYSACCHARIDE;

ARTICLE; CATTLE; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; PROTEIN SECRETION; SUBMANDIBULAR GLAND; CHEMISTRY;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; EDETIC ACID; HYDRAZINES; POLYSACCHARIDES;

EID: 84897398757     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.02.030     Document Type: Article

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