An analytical and structural database provides a strategy for sequencing O-glycans from microgram quantities of glycoproteins

Analytical Biochemistry

Volumn 304, Issue 1, 2002, Pages 70-90

  Royle, Louise ^a   Mattu, Taj S ^a   Hart, Edmund ^a   Langridge, James I ^b   Merry, Anthony H ^a   Murphy, Neil ^a   Harvey, David J ^a   Dwek, Raymond A ^a   Rudd, Pauline M ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b MICROMASS UK LTD   (United Kingdom)

Author keywords

High performance liquid chromatography; Liquid chromatography mass spectrometry mass spectrometry; Matrix assisted laser desorption ionization mass spectrometry; O glycans; Sugar sequencing

Indexed keywords

DATABASE SYSTEMS; GLYCOPROTEINS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID SUGAR; MAMMALS; MASS SPECTROMETRY;

ANALYTICAL PARAMETERS; LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIES; MATRIX ASSISTED LASER DESORPTION; MONOSACCHARIDE COMPOSITION; O-GLYCANS; REVERSED PHASE HPLC; STRUCTURAL DATABASE; THREE-DIMENSIONAL SHAPE;

POLYSACCHARIDES;

FETUIN; GELATINASE B; GLYCAN; GLYCOPEPTIDE; GLYCOPHORIN A; GLYCOPROTEIN; GLYCOSIDASE; IMMUNOGLOBULIN A1; MONOSACCHARIDE; SECRETORY IMMUNOGLOBULIN; SYNTHETIC PEPTIDE;

ANALYTICAL PARAMETERS; ARTICLE; CHEMICAL COMPOSITION; CHEMICAL STRUCTURE; CONTROLLED STUDY; DATA BASE; ELUTION; HUMAN; HYDROPHOBICITY; IMMUNOGLOBULIN BLOOD LEVEL; MASS FRAGMENTOGRAPHY; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

BOVINAE;

EID: 0036571275     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5619     Document Type: Article

References (62)

1. Why mammalian cell surface proteins are glycoproteins
2. On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database
3. Cinderella's Coach is-ready
4. The glycosylation of the complement regulatory protein, human erythrocyte CD59
5. Primary structure of twenty three neutral and monosialylated oligosaccharides O-glycosidically linked to the human secretory immunoglobulin A hinge region determined by a combination of permethylation analysis and 400-MHz 1H-NMR spectroscopy
6. Concepts and principles of O-linked glycosylation
7. Assembly of asparagine-linked oligosaccharides
8. O-Glycosylation of the mucin type
9. A rapid high-resolution high-performance liquid chromatographic method for separating glycan mixtures and analyzing oligosaccharide profiles
10. Identification method for twelve oligomannose-type sugar chains thought to be processing intermediates of glycoproteins
11. Analyses of N-linked oligosaccharides using a two-dimensional mapping technique
12. High-sensitivity and high-resolution methods for glycoprotein analysis
13. High resolution and high sensitivity methods for oligosaccharide mapping and characterization by normal phase high performance liquid chromatography following derivatization with highly fluorescent anthranilic acid
14. Analysis of pyridylaminated O-linked sugar chains by two-dimensional sugar mapping
15. Glycoprotein structure determination by mass spectrometry
16. Carbohydrate-mediated recognition systems in innate immunity
17. Analysis of carbohydrate heterogeneity in a glycoprotein using liquid chromatography/mass spectrometry and liquid chromatography with tandem mass spectrometry
18. Selective glycopeptide mapping of erythropoietin by on-line high-performance liquid chromatography-electrospray ionization mass spectrometry
19. In vivo glycosylation of mucin tandem repeats
20. Liquid chromatography-electrospray mass spectrometry as a tool for the analysis of sulfated oligosaccharides from mucin glycoproteins
21. Sequencing of sulfated oligosaccharides from mucins by liquid chromatography and electrospray ionization tandem mass spectrometry
22. Structural analysis of murine zona pellucida glycans: Evidence for the expression of core 2-type O-glycans and the Sd(a) antigen
23. The glycosylation and structure of human serum IgA1, Fab, and Fc regions and the role of N-glycosylation on Fc alpha receptor interactions
24. Complete enzymic synthesis of the mucin-type sialyl Lewis x epitope, involved in the interaction between PSGL-1 and P-selectin
25. The beta 1-2-D-xylose and alpha 1-3-L-fucose substituted N-linked oligosaccharides from Erythrina cristagalli lectin: Isolation, characterisation and comparison with other legume lectins
26. Use of hydrazine to release in intact and unreduced form both N- and O-linked oligosaccharides from glycoproteins
27. Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid
28. Oligosaccharide sequencing technology
29. Identification of highly fucosylated N-linked oligosaccharides from the human parotid gland
30. Analytical and preparative separation of anionic oligosaccharides by weak anion-exchange high-performance liquid chromatography on an inert polymer column
31. Sialylated N-glycans in adult rat brain tissue: A widespread distribution of disialylated antennae in complex and hybrid structures
32. Quantitative aspects of the matrix-assisted laser desorption mass spectrometry of complex oligosaccharides
33. A comparison of the results of sequential hydrazinolysis-nitrosation and alkali-mediated cleavage-nitrosation of the O-linked oligosaccharides of gastric mucus glycoproteins
34. Release of O-linked sugar chains from glycoproteins with anhydrous hydrazine and pyridylamination of the sugar chains with improved reaction conditions
35. Microanalysis of glycosaminoglycan-derived oligosaccharides labeled with a fluorophore 2-aminobenzamide by high-performance liquid chromatography: Application to disaccharide composition analysis and exosequencing of oligosaccharides
36. Structural analyses of asparagine-linked oligosaccharides of porcine pancreatic kallikrein
37. Contribution of component monosaccharides to the coordinates of neutral and sialyl pyridylaminated N-glycans on a two-dimensional sugar map
38. Two-dimensional elution map of GalNAc-containing N-linked oligosaccharides
39. Characterization of carbohydrates using highly fluorescent 2-aminobenzoic acid tag following gel electrophoresis of glycoproteins
40. Sequencing of N-linked oligosaccharides directly from protein gels: In-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography
41. A sensitive mapping strategy for monitoring the reproducibility of glycan processing in an HIV vaccine, RGP-160, expressed in a mammalian cell line
42. Presence of an O-glycosidically linked hexasaccharide in fetuin
43. Structure of the O-glycosidically linked carbohydrate units of fetuin
44. Structural studies on human erythrocyte glycoproteins: Alkali-labile oligosaccharides
45. O-Glycan analysis of natural human neutrophil gelatinase B using a combination of normal phase-HPLC and online tandem mass spectrometry: Implications for the domain organization of the enzyme
46. Newcastle disease virus contains a linkage-specific glycoprotein sialidase: Application to the localization of sialic acid residues in N-linked oligosaccharides of alpha 1-acid glycoprotein
47. Comparative study of the mucin-type sugar chains of human chorionic gonadotropin present in the urine of patients with trophoblastic diseases and healthy pregnant women
48. Biosynthesis of blood group i-active polylactosaminoglycans: Partial purification and properties of an UDP-GlcNAc:N-acetyllactosaminide beta 1-3-N-acetylglucosaminyltransferase from Novikoff tumor cell ascites fluid
49. Structures of neutral O-linked polylactosaminoglycans on human skim milk mucins: A novel type of linearly extended poly-N-acetyllactosamine backbones with Gal beta(1-4)GlcNAc beta(1-6) repeating units
50. Biosynthesis of branched polylactosaminoglycans: Embryonal carcinoma cells express midchain beta 1,6-N-acetylglucosaminyltransferase activity that generates branches to preformed linear backbones
51. Structures of the sugar chains of interferon-gamma produced by human myelomonocyte cell line HBL-38
52. A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates
53. "internal residue loss": Rearrangements occurring during the fragmentation of carbohydrates derivatized at the reducing terminus
54. Oligosaccharide characterization using collision-induced dissociation fast atom bombardment mass spectrometry: Evidence for internal monosaccharide residue loss
55. Loss of internal 1-6 substituted monosaccharide residues from underivatized and per-O-methylated trisaccharides
56. Sodium-cationized oligosaccharides do not appear to undergo 'internal residue loss' rearrangement processes on tandem mass spectrometry
57. False sugar sequence in electrospray tandem mass spectrometry of underivatised sialyl-Lewis-type oligosaccharides
58. Observation of internal monosaccharide losses in the collisionally activated dissociation mass spectra of anthracycline aminodisaccharides
59. Conformation-independent binding of monoglucosylated ribonuclease B to calnexin
60. Recovery of intact 2-aminobenzamide-labeled O-glycans released from glycoproteins by hydrazinolysis