Novel vector systems optimized for injecting in vitro-synthesized mRNA into zebrafish embryos

Molecules and Cells

Volumn 17, Issue 2, 2004, Pages 373-376

  Ro, Hyunju ^a   Soun, Kyungchull ^a   Kim, Eun Jung ^a   Rhee, Myungchull ^a  

^a Chungnam National University   (South Korea)

Author keywords

Microinjection; pcGlobin2; pcGlobin2 GST; Zebrafish

Indexed keywords

BETA GLOBIN; MESSENGER RNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; EMBRYO; EMBRYO DEVELOPMENT; GENE SEQUENCE; GENETIC TRANSFECTION; IMMUNOBLOTTING; IN SITU HYBRIDIZATION; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN BINDING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA STABILITY; RNA SYNTHESIS; RNA TRANSCRIPTION; ZEBRA FISH; ANIMAL; GENE VECTOR; GENETICS; HISTOLOGY; METABOLISM; METHODOLOGY; MICROINJECTION; PRENATAL DEVELOPMENT; REPORTER GENE;

ANIMALIA; DANIO RERIO;

ANIMALS; GENES, REPORTER; GENETIC VECTORS; IN SITU HYBRIDIZATION; MICROINJECTIONS; RNA, MESSENGER; TRANSFECTION; ZEBRAFISH;

EID: 3442894747     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Alike, W. V., Harry, O. V., and Adri, A. M. T. (2001) Vector design for optimal protein expression. BioTechniques 31, 572-582.
2. Barik, S. and Galinsky, M. (1991) "Megaprimer" method of PCR: increase template concentration improves yield. BioTechniques 10, 489-490.
3. Chan, F. Y., Robinson, J., Brownlie, A., Shivdasani, R. A., Donovan, A., Brugnara, C., Kim, J., Lau, B. C., Witkowska, E., and Zon, L. I. (1997) Characterization of adult α- and β-globin genes in zebrafish. Blood 89, 688-700.
4. Hyatt, T. N. and Ekker, S. C. (1999) Vectors and techniques for ectopic gene expression in zebrafish; in Methods in Cell Biology, Detrich, H. W., Westerfield, M., and Zon, L. I. (eds.), Vol. 59, pp. 117-126, Academic Press.
5. Kozak, M. (1987) An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15, 8125-8148.
6. Tago, K., Nakamura, T., Nishita, M., Hyodo, J., Nagai, S., Murata, Y., Adachi, S., Ohwada, S., Morishita, Y., Shibuya, H., and Akiyama, T. (2000) Inhibition of Wnt signaling by ICAT, a novel β-catenin-interacting protein. Genes Dev. 14, 1741-1749
7. Thisse, C., Thisse, B., Schilling, T. F., and Postlethwait, J. H. (1993) Structure of the zebrafish snail gene and its expression in wild-type, spadtail and no tail mutant embryos. Development 119, 1203-1215.
8. Turner, D. L. and Weintraub, H. (1994) Expression of achaetescute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate. Genes Dev. 8, 1434-1447.
9. Wahle, E. and Kuhn, U. (1997) The mechanism of 3′ cleavage and polyadenylation of 3′ eukaryotic pre-mRNA. Prog. Nucleic Acid Res. Mol. Biol. 57, 41-71.
10. Westerfield, M. (1995) General methods for zebrafish care. Zebrafish book: a guide for the laboratory use of zebrafish (Danio rerio), University of Oregon, Eugene, OR.