The fluorescent two-hybrid assay to screen for protein-protein interaction inhibitors in live cells: Targeting the interaction of p53 with Mdm2 and Mdm4

Journal of Biomolecular Screening

Volumn 19, Issue 4, 2014, Pages 516-525

  Yurlova, Larisa ^a   Derks, Maarten ^b   Buchfellner, Andrea ^c   Hickson, Ian ^b   Janssen, Marc ^b   Morrison, Denise ^b   Stansfield, Ian ^b   Brown, Christopher J ^d   Ghadessy, Farid J ^d   Lane, David P ^d   Rothbauer, Ulrich ^c   Zolghadr, Kourosh ^a   Krausz, Eberhard ^b  

^a CHROMOTEK GMBH   (Germany)

^b JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

^c UNIVERSITY OF TÜBINGEN   (Germany)

^d AGENCY FOR SCIENCE   (Singapore)

Author keywords

Fluorescent two hybrid (F2H) assay; Hdm2; HdmX; Mdm2; Mdm4; p53; protein protein interaction screen

Indexed keywords

CARBAMAZEPINE; LOPERAMIDE; NUTLIN 3; PROTEIN MDM2; PROTEIN MDMX; PROTEIN P53; TISSUE FACTOR PATHWAY INHIBITOR;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL MEMBRANE PERMEABILITY; CELL STRAIN BHK; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; DRUG PENETRATION; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE; FLUORESCENT TWO HYBRID ASSAY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SCREENING; TWO HYBRID SYSTEM;

FLUORESCENT TWO-HYBRID (F2H) ASSAY; HDM2; HDMX; MDM2; MDM4; P53; PROTEIN-PROTEIN INTERACTION SCREEN;

ANIMALS; CELL LINE; CELL SURVIVAL; CRICETINAE; DRUG DISCOVERY; DRUG EVALUATION, PRECLINICAL; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; PROTEIN BINDING; PROTO-ONCOGENE PROTEINS C-MDM2; REPRODUCIBILITY OF RESULTS; SMALL MOLECULE LIBRARIES; TUMOR SUPPRESSOR PROTEIN P53; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 84896753052     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057113518067     Document Type: Article

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