Redundancy in the periplasmic adaptor proteins AcrA and AcrE provides resilience and an ability to export substrates of multidrug efflux

Journal of Antimicrobial Chemotherapy

Volumn 69, Issue 4, 2014, Pages 982-987

  Smith, Helen E ^a   Blair, Jessica M A ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Antibiotic resistance; Drug targets; Efflux pumps; Multidrug resistance; RND; Salmonella spp.

Indexed keywords

ACRA PROTEIN; ACRE PROTEIN; ACRIFLAVINE; ADAPTOR PROTEIN; BACTERIAL PROTEIN; CHLORAMPHENICOL; CIPROFLOXACIN; ETHIDIUM BROMIDE; FUSIDIC ACID; NALIDIXIC ACID; NORFLOXACIN; PERIPLASMIC PROTEIN; TETRACYCLINE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM MUTANT; CELL ADHESION; CELL INVASION; CONTROLLED STUDY; ENZYME SPECIFICITY; FLUOROMETRY; INTESTINE EPITHELIUM CELL; MULTIDRUG RESISTANCE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN TARGETING; SALMONELLA TYPHIMURIUM; SCANNING ELECTRON MICROSCOPY;

ANTIBIOTIC RESISTANCE; DRUG TARGETS; EFFLUX PUMPS; MULTIDRUG RESISTANCE; RND; SALMONELLA SPP.;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; GENE DELETION; GENETIC COMPLEMENTATION TEST; HUMANS; MEMBRANE TRANSPORT PROTEINS; MICROBIAL SENSITIVITY TESTS; SALMONELLA TYPHIMURIUM;

EID: 84896474135     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkt481     Document Type: Article

References (24)

1. Howell L.Global Risks2013:Aninitiative of the RiskResponseNetwork.http://reports.weforum.org/global-risks-2013/(28 October 2013, date last accessed).
2. Buckley AM, Webber MA, Cooles S et al. The AcrAB-TolC efflux system of Salmonella enterica serovar Typhimuriumplays a role in pathogenesis. Cell Microbiol 2006; 8: 847-56.
3. Nishino K, Latifi T, Groisman EA. Virulence and drug resistance roles of multidrug efflux systems of Salmonella enterica serovar Typhimurium. Mol Microbiol 2006; 59: 126-41.
4. Blair JM, La Ragione RM, Woodward MJ et al. Periplasmic adaptor protein AcrA has a distinct role in the antibiotic resistance and virulence of Salmonella enterica serovar Typhimurium. J Antimicrob Chemother 2009; 64: 965-72.
5. Touze T, Eswaran J, Bokma E et al. Interactions underlying assembly of the Escherichia coli AcrAB-TolC multidrug efflux system. Mol Microbiol 2004; 53: 697-706.
6. Lobedanz S, Bokma E, Symmons M et al. A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug efflux pumps. Proc Natl Acad Sci USA 2007; 104: 4612-7.
7. Mikolosko J, Bobyk K, Zgurskaya HI et al. Conformational flexibility in the multidrug efflux system protein AcrA. Structure 2006; 14: 577-87.
8. Balakrishnan L, Hughes C, Koronakis V. Substrate-triggered recruitment of the TolC channel-tunnel during type I export of hemolysin by Escherichia coli. J Mol Biol 2001; 313: 501-10.
9. Borges-Walmsley MI, Beauchamp J, Kelly SM et al. Identification of oligomerization and drug-binding domains of the membrane fusion protein EmrA. J Biol Chem 2003; 278: 12903-12.
10. Bagai I, Liu W, Rensing C et al. Substrate-linked conformational change in the periplasmic component of a Cu(I)/Ag(I) efflux system. J Biol Chem 2007; 282: 35695-702.
11. Aires JR, Nikaido H. Aminoglycosides are captured from both periplasm and cytoplasm by the AcrD multidrug efflux transporter of Escherichia coli. J Bacteriol 2005; 187: 1923-9.
12. Zgurskaya HI, Nikaido H. AcrA is a highly asymmetric protein capable of spanning the periplasm. J Mol Biol 1999; 285: 409-20.
13. Janganan TK, Bavro VN, Zhang L et al. Evidence for the assembly of a bacterial tripartite multidrug pump with a stoichiometry of 3:6:3. J Biol Chem 2011; 286: 26900-12.
14. Piddock LJ. Multidrug-resistance efflux pumps-not just for resistance. Nat Rev Microbiol 2006; 4: 629-36.
15. Webber MA, Bailey AM, Blair JMA et al. The global consequence of disruption of the AcrAB-TolC efflux pump in Salmonella enterica includes reduced expression of SPI-1 and other attributes required to infect the host. J Bacteriol 2009; 191: 4276-85.
16. Yamasaki S, Nagasawa S, Hayashi-Nishino M et al. AcrA dependency of the AcrD effluxpumpin Salmonella enterica serovar Typhimurium. J Antibiot 2011; 64: 433-7.
17. Wray C, Sojka W. Experimental Salmonella typhimurium infection in calves. Res Vet Sci 1978; 25: 139-43.
18. Datsenko KA,Wanner BL. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 2000; 97: 6640-5.
19. Lau SY, Zgurskaya HI. Cell division defects in Escherichia coli deficient in the multidrug efflux transporter AcrEF-TolC. J Bacteriol 2005; 187: 7815-25.
20. Andrews J. BSAC Methods for Antimicrobial Susceptibility Testing. 2006. http://bsac.org.uk/wp-contents/uploads/2012/02/version_5_.pdf (25 November 2013, date last accessed).
21. Coldham NG, Webber M, Woodward MJ et al. A96-wellplatefluorescence assay for assessment of cellular permeability and active efflux in Salmonella enterica serovar Typhimurium and Escherichia coli. J Antimicrob Chemother 2010; 65: 1655-63.
22. Mortimer PGS, Piddock LJV. A comparison of methods used for measuring the accumulation of quinolones by Enterobacteriaceae, Pseudomonas aeruginosa and Staphylococcus aureus. J Antimicrob Chemother 1991; 28: 639-53.
23. Piddock LJV, Ricci V. Accumulation of five fluoroquinolones by Mycobacterium tuberculosis H37Rv. J Antimicrob Chemother 2001; 48: 787-91.
24. Dibb-Fuller MP, Allen-Vercoe E, Thorns CJ et al. Fimbriae-and flagella-mediated association with and invasion of cultured epithelial cells by Salmonella enteritidis. Microbiology 1999; 145: 1023-31.