A novel and rapid loop-mediated isothermal amplification assay for the specific detection of Verticillium dahliae

Journal of Applied Microbiology

Volumn 116, Issue 4, 2014, Pages 942-954

  Moradi, A ^a   Almasi, M A ^a   Jafary, H ^b   Mercado Blanco, J ^c  

^a UNIVERSITY OF ZANJAN   (Iran)

^b Zanjan Agricultural and Natural Resource Research Center   (Iran)

^c INSTITUTO DE AGRICULTURA SOSTENIBLE   (Spain)

Author keywords

Loop mediated isothermal amplification; Nested PCR; Olive; RAPD marker; Soilborne; Verticillium dahliae

Indexed keywords

AGRICULTURE; DNA; FUNGI; ISOTHERMS; PATHOGENS; POLYMERASE CHAIN REACTION; PURIFICATION; SOIL SURVEYS; SOILS;

AGRICULTURAL SOILS; DNA PURIFICATION; LOOP MEDIATED ISOTHERMAL AMPLIFICATIONS; LOOP-MEDIATED ISOTHERMAL AMPLIFICATIONS; NESTED PCR; OLIVE; RAPD MARKERS; SOIL SAMPLE; SOILBORNE; VERTICILLIUM DAHLIAE;

ASSAYS;

FUNGAL DNA; PRIMER DNA;

BIOASSAY; COST-BENEFIT ANALYSIS; DISEASE CONTROL; DISEASE INCIDENCE; DNA; FUNGUS; PATHOGEN; POLYMERASE CHAIN REACTION; VASCULAR PLANT;

AGRICULTURE; ARTICLE; CROSS REACTION; FUNGAL DETECTION; FUNGUS GROWTH; INTERMETHOD COMPARISON; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIVE; ORCHARD; PATHOTYPE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOIL MICROFLORA; VERTICILLIUM DAHLIAE; WILTING; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEIC ACID AMPLIFICATION; PROCEDURES; RANDOM AMPLIFIED POLYMORPHIC DNA; VERTICILLIUM;

FUNGI; VERTICILLIUM DAHLIAE;

DNA PRIMERS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE; SENSITIVITY AND SPECIFICITY; SOIL MICROBIOLOGY; VERTICILLIUM;

EID: 84896127397     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12407     Document Type: Article

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