A protein engineered to bind uranyl selectively and with femtomolar affinity

Nature Chemistry

Volumn 6, Issue 3, 2014, Pages 236-241

  Zhou, Lu ^a   Bosscher, Mike ^a   Zhang, Changsheng ^b,c   Özçubukçu, Salih ^a   Zhang, Liang ^a   Zhang, Wen ^a   Li, Charles J ^a   Liu, Jianzhao ^a   Jensen, Mark P ^d   Lai, Luhua ^b,c   He, Chuan ^a  

^a UNIVERSITY OF CHICAGO   (United States)

^b PEKING UNIVERSITY   (China)

^c PEKING UNIVERSITY   (China)

^d ARGONNE NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

METAL NANOPARTICLE; PROTEIN; URANIUM;

ARTICLE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; METABOLISM; PROTEIN ENGINEERING;

BINDING SITES; METAL NANOPARTICLES; MODELS, MOLECULAR; PROTEIN ENGINEERING; PROTEINS; URANIUM;

EID: 84895165932     PISSN: 17554330     EISSN: 17554349     Source Type: Journal    
DOI: 10.1038/nchem.1856     Document Type: Article

References (37)

1. Nozaki, Y. A fresh look at element distribution in the North Pacific. Eos Trans. Am. Geophys. Union 78, 221-227 (1997).
2. Tabushi, I., Kobuke, Y. & Nishiya, T. Extraction of uranium from seawater by polymer-bound macrocyclic hexaketone. Nature 280, 665-666 (1979).
3. Kanno, M. Present status of study on extraction of uranium from sea water. J. Nucl. Sci. Technol. 21, 1-9 (1984). (Pubitemid 14572384)
4. Egawa, H., Nonaka, T. & Nakayama, M. Recovery of uranium from seawater. 7. Concentration and separation of uranium in acidic eluate. Ind. Eng. Chem. Res. 29, 2273-2277 (1990).
5. Sather, A. C., Berryman, O. B., Ajami, D. & Rebek, J. Jr Selective recognition and extraction of the uranyl ion. J. Am. Chem. Soc. 132, 13572-13574 (2010).
6. Franczyk, T. S., Czerwinski, K. R. & Raymond, K. N. Stereognostic coordination chemistry. 1. The design and synthesis of chelators for the uranyl ion. J. Am. Chem. Soc. 114, 8138-8146 (1992).
7. Gordon, A. E., Xu, J., Raymond, K. N. & Durbin, P. Rational design of sequestering agents for plutonium and other actinides. Chem. Rev. 103, 4207-4282 (2003).
8. Gong, H. Y., Rambo, B. M., Karnas, E., Lynch, V. M. & Sessler, J. L. A 'Texas-sized' molecular box that forms an anion-induced supramolecular necklace. Nature Chem. 2, 406-409 (2010).
9. Wegner, S. V., Boyaci, H., Chen, H., Jensen, M. P. & He, C. Engineering a uranyl-specific binding protein from NikR. Angew. Chem. Int. Ed. 48, 2339-2341 (2009).
10. 2+): development and comparison of labeled and label-free DNAzyme-gold nanoparticle systems. J. Am. Chem. Soc. 130, 14217-14226 (2008).
11. LeClainche, L. & Vita, C. Selective binding of uranyl cation by a novel calmodulin peptide. Environ. Chem. Lett. 4, 45-49 (2006).
12. Manos M. J. & Kanatzidis M. G. Layered metal sulfides capture uranium from seawater. J. Am. Chem. Soc., 134 (39), 16441-16446 (2012).
13. Carboni, M., Abney, C. W., Liu, S. & Lin, W. Highly porous and stable metal-organic frameworks for uranium extraction. Chem. Sci. 4, 2396-2402 (2013).
14. Changlea, A. et al. Molecular basis of metal-ion selectivity and zeptomolar sensitivity by Cue R. Science 301, 1383-1387 (2003).
15. Aisen, P., Leibman, A. & Zweier, J. Stoichiometric and site characteristics of the binding of iron to human transferrin. J. Biol. Chem. 253, 1930-1937 (1978). (Pubitemid 8299518)
16. Banci, L. et al. Affinity gradients drive copper to cellular destinations. Nature 465, 367-368 (2010).
17. Georgiou, G. et al. Display of beta-lactamase on the Escherichia coli surface: outer membrane phenotypes conferred by Lpp′-OmpA′-beta- lactamase fusions. Protein Eng. 9, 239-247 (1996).
18. Zhang, C. & Lai, L. Automatch: target-binding protein design and enzyme design by automatic pinpointing potential active sites in available protein scaffolds. Proteins 80, 1078-1094 (2012).
19. Pible, O., Guilbaud, P., Pellequer, J. L., Vidaud, C. & Quéméneur, E. Biochimie 88, 1631-1638 (2006).
20. Zeikus, J. G. & Wolee, R. S. Methanobacterium thermoautotrophicus sp. n., an anaerobic, autotrophic, extreme thermophile. J. Bacteriol. 109, 707-715 (1972).
21. Saito, K. & Miyauchi, T. Chemical forms of uranium in artificial seawater. J. Nucl. Sci. Technol. 19, 145-150 (1982).
22. Regan, L. & DeGrado, W. F. Characterization of a helical protein designed from first principles. Science 241, 976-978 (1988).
23. Lovejoy, B. et al. Crystal structure of a synthetic triple-stranded alpha-helical bundle. Science 259, 1288-1293 (1993). (Pubitemid 23100266)
24. Wendt, H., Berger, C., Baici, A., Thomas, R. M. & Bosshard, H. R. Kinetics of folding of leucine zipper domains. Biochemistry 34, 4097-4107 (1995).
25. Pordea, A. & Ward, T. R Artificial metalloenzymes: combining the best features of homogenous and enzymatic catalysis. Synlett 20, 3225-3236 (2009).
26. DeGrado, W. F., Summa, C. M., Pavone, V., Nastri, F. & Lombardi, A. De novo design and structural characterization of proteins and metalloproteins. Annu. Rev. Biochem. 68, 779-819 (1999). (Pubitemid 29449209)
27. Dutton, P. L. & Moser, C. C. Engineering enzymes. Faraday Discuss. 148, 443-448 (2011).
28. Khare, S. D. et al. Computational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysis. Nature Chem. Biol. 8, 294-300 (2012).
29. Azoitei, M. L. et al. Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold. Science 334, 373-376 (2011).
30. Radford, R. J., Brodin, J. D., Salgado, E. N. & Tezcan, A. Expanding the utility of proteins as platforms for coordination chemistry. Coord. Chem. Rev. 225, 790-803 (2011).
31. Brodin, J. D. et al. Metal-directed, chemically tunable assembly of one-, two- and three-dimensional crystalline protein arrays. Nature Chem. 4, 375-382 (2012).
32. Saven, J. G. Computational protein design: engineering molecular diversity, nonnatural enzymes, nonbiological cofactor complexes, and membrane proteins. Curr. Opin. Chem. Biol. 15, 452-457 (2011).
33. Privett, H. K. et al. Iterative approach to computational enzyme design. Proc. Natl Acad. Sci. USA 109, 3790-3795 (2012).
34. Liu, S. et al. Nonnatural protein-protein interaction-pair design by key residues grafting. Proc. Natl Acad. Sci. USA 104, 5330-5335 (2007). (Pubitemid 47175680)
35. Fleishman, S. J. et al. Computational design of proteins targeting the conserved stem region of influenza hemagglutinin. Science 332, 816-821 (2011).
36. Lu, Y., Yeung, N., Sieracki, N. & Marshall, N. M. Design of functional metalloproteins. Nature 460, 855-862 (2009).
37. Touw, D. S., Nordman, C. E., Stuckey, J. A. & Pecoraro, V. L. Identifying important structural characteristics of arsenic resistance proteins by using designed three stranded coils. Proc. Natl Acad. Sci. USA 104, 11969-11974 (2007). (Pubitemid 47185614)