Single-step generation of gene knockout-rescue system in pluripotent stem cells by promoter insertion with CRISPR/Cas9

Biochemical and Biophysical Research Communications

Volumn 444, Issue 2, 2014, Pages 158-163

  Matsunaga, Taichi ^b   Yamashita, Jun K ^a  

^a KYOTO UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

CRISPR Cas9 system; Endothelial cell differentiation; Knockout rescue system; Promoter; VEGFR2 Flk1

Indexed keywords

CRISPR/CAS9 SYSTEM; ENDOTHELIAL CELL DIFFERENTIATION; KNOCKOUT-RESCUE SYSTEM; PROMOTER; VEGFR2/FLK1;

ANIMALS; CELL DIFFERENTIATION; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; CYTOMEGALOVIRUS; DOXYCYCLINE; EMBRYONIC STEM CELLS; ENDOTHELIAL CELLS; GENE EXPRESSION; GENE KNOCKOUT TECHNIQUES; IMMUNOBLOTTING; MICE; MODELS, GENETIC; MUTAGENESIS, INSERTIONAL; PLURIPOTENT STEM CELLS; PROMOTER REGIONS, GENETIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TETRACYCLINE; VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2;

EID: 84894588307     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2014.01.037     Document Type: Article

References (26)

1. B. Wiedenheft, S.H. Sternberg, and J.A. Doudna RNA-guided genetic silencing systems in bacteria and archaea Nature 482 2012 331 338
2. M. Jinek, K. Chylinski, and I. Fonfara et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity Science 337 2012 816 821
3. L. Cong, F.A. Ran, and D. Cox et al. RNA-guided genetic silencing systems in bacteria and archaea Science 339 2013 819 823
4. P. Mali, L. Yang, and K.M. Esvelt et al. RNA-guided human genome engineering via Cas9 Science 339 2013 823 826
5. W.Y. Hwang, Y. Fu, D. Reyon, and M.L. Maeder et al. Efficient genome editing in zebrafish using a CRISPR-Cas system Nat. Biotechnol. 31 2013 227 229
6. B. Shen, J. Zhang, and H. Wu et al. Generation of gene-modified mice via Cas9/RNA-mediated gene targeting Cell Res. 23 2013 720 723
7. Q. Ding, S.N. Regan, and Y. Xia et al. Enhanced efficiency of human pluripotent stem cell genome editing through replacing TALENs with CRISPRs Cell Stem Cell 12 2013 393 394
8. F. Shalaby, J. Ho, and W.L. Stanford et al. A requirement for Flk1 in primitive and definitive hematopoiesis and vasculogenesis Cell 89 1997 981 990
9. J. Yamashita, H. Itoh, and M. Hirashima et al. Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors Nature 408 2000 92 96
10. T. Yurugi-Kobayashi, H. Itoh, and T. Schroeder et al. Adrenomedullin/cyclic AMP pathway induces Notch activation and differentiation of arterial endothelial cells from vascular progenitors Arterioscler. Thromb. Vasc. Biol. 26 2006 1977 1984
11. K. Yamamizu, S. Furuta, and S. Katayama et al. The kappa opioid system regulates endothelial cell differentiation and pathfinding in vascular development Blood 118 2011 775 785
12. K. Yamamizu, K. Kawasaki, and S. Katayama et al. Enhancement of vascular progenitor potential by protein kinase A through dual induction of Flk-1 and Neuropilin-1 Blood 114 2009 3707 3716
13. K. Yamamizu, T. Matsunaga, and S. Katayama et al. PKA/CREB signaling triggers initiation of endothelial and hematopoietic cell differentiation via Etv2 induction Stem Cells 30 2012 687 696
14. S.I. Nishikawa, S. Nishikawa, and M. Hirashima et al. Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages Development 125 1998 1747 1757
15. T. Era, and O.N. Witte Regulated expression of P210 Bcr-Abl during embryonic stem cell differentiation stimulates multipotential progenitor expansion and myeloid cell fate Proc. Natl. Acad. Sci. USA 97 2000 1737 1742
16. J.K. Yamashita, M. Takano, and M. Hiraoka-Kanie et al. Prospective identification of cardiac progenitors by a novel single cell-based cardiomyocyte induction FASEB J. 19 2005 1534 1536
17. A. Kappel, V. Rönicke, and A. Damert et al. Identification of vascular endothelial growth factor (VEGF) receptor-2 (Flk-1) promoter/enhancer sequences sufficient for angioblast and endothelial cell-specific transcription in transgenic mice Blood 93 1999 4284 4292
18. A. Kappel, T.M. Schlaeger, and I. Flamme et al. Role of SCL/Tal-1, GATA, and ets transcription factor binding sites for the regulation of flk-1 expression during murine vascular development Blood 96 2000 3078 3085
19. F.A. Ran, P.D. Hsu, and J. Wright et al. Genome engineering using the CRISPR-Cas9 system Nat. Protoc. 8 2013 2281 2308
20. T.P. Yamaguchi, D.J. Dumont, and R.A. Conlon et al. Flk-1, an flt-related receptor tyrosine kinase is an early marker for endothelial cell precursors Development 118 1993 489 498
21. L.S. Qi, M.H. Larson, and L.A. Gilbert et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression Cell 152 2013 1173 1183
22. D. Bikard, W. Jiang, and P. Samai et al. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system Nucleic Acids Res. 41 2013 7429 7437
23. L.A. Gilbert, M.H. Larson, and L. Morsut et al. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes Cell 154 2013 442 451
24. M.L. Maeder, S.J. Linder, and V.M. Cascio et al. CRISPR RNA-guided activation of endogenous human genes Nat. Methods 10 2013 977 979
25. P. Perez-Pinera, D.D. Kocak, and C.M. Vockley et al. RNA-guided gene activation by CRISPR-Cas9-based transcription factors Nat. Methods 10 2013 973 976
26. A.W. Cheng, H. Wang, and H. Yang et al. Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system Cell Res. 23 2013 1163 1171