pH gradient and distribution of streptococci, lactobacilli, prevotellae, and fusobacteria in carious dentine

Clinical Oral Investigations

Volumn 18, Issue 2, 2014, Pages 659-669

  Kianoush, Nima ^a,b,c   Nguyen, Ky Anh T ^a,b   Browne, Gina V ^a   Simonian, Mary ^a   Hunter, Neil ^a,b  

^a WESTMEAD HOSPITAL   (Australia)

^b UNIVERSITY OF SYDNEY   (Australia)

^c Level 2 Westmead Center for Oral Health C24   (Australia)

Author keywords

16S rRNA; Caries; Gram negative bacteria; Gram positive bacteria; pH gradient; Real time PCR

Indexed keywords

BACTERIUM; CLASSIFICATION; DENTAL CARIES; DENTIN; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; PH;

BACTERIA; DENTAL CARIES; DENTIN; HUMANS; HYDROGEN-ION CONCENTRATION;

EID: 84894562578     PISSN: 14326981     EISSN: 14363771     Source Type: Journal    
DOI: 10.1007/s00784-013-1009-0     Document Type: Article

References (69)

1. Featherstone JD (2004) The continuum of dental caries--evidence for a dynamic disease process. J Dent Res 83 Spec No C: C39-42.
2. van Houte J (1994) Role of microorganisms in caries etiology. J Dent Res 73(3): 672-681.
3. Chhour KL, Nadkarni MA, Byun R, Martin FE, Jacques NA, Hunter N (2005) Molecular analysis of microbial diversity in advanced caries. J Clin Microbiol 43(2): 843-849. doi: 10. 1128/JCM. 43. 2. 843-849. 2005.
4. Nadkarni MA, Caldon CE, Chhour KL, Fisher IP, Martin FE, Jacques NA, Hunter N (2004) Carious dentine provides a habitat for a complex array of novel Prevotella-like bacteria. J Clin Microbiol 42(11): 5238-5244. doi: 10. 1128/JCM. 42. 11. 5238-5244. 2004.
5. Takahashi N, Saito K, Schachtele CF, Yamada T (1997) Acid tolerance and acid-neutralizing activity of Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum. Oral Microbiol Immunol 12(6): 323-328.
6. Marsh PD (1994) Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res 8(2): 263-271.
7. Takahashi N, Nyvad B (2011) The role of bacteria in the caries process: ecological perspectives. J Dent Res 90(3): 294-303. doi: 10. 1177/0022034510379602.
8. Stephan RM (1940) Two factors of possible importance in relation to the etiology and treatment of dental caries and other dental diseases. Science 92(2399): 578-579. doi: 10. 1126/science. 92. 2399. 578.
9. Featherstone JD, Rodgers BE (1981) Effect of acetic, lactic, and other organic acids on the formation of artificial carious lesions. Caries Res 15(5): 377-385.
10. Macgregor AB (1961) The position and extent of acid in the carious process. Archives of oral biology 4: 86-91.
11. van Houte J, Sansone C, Joshipura K, Kent R (1991) Mutans streptococci and non-mutans streptococci acidogenic at low pH and in vitro acidogenic potential of dental plaque in two different areas of the human dentition. J Dent Res 70(12): 1503-1507.
12. Takahashi N, Yamada T (1999) Acid-induced acid tolerance and acidogenicity of non-mutans streptococci. Oral Microbiol Immunol 14(1): 43-48.
13. Iwami Y, Abbe K, Takahashi-Abbe S, Yamada T (1992) Acid production by streptococci growing at low pH in a chemostat under anaerobic conditions. Oral Microbiol Immunol 7(5): 304-308.
14. Badet MC, Richard B, Dorignac G (2001) An in vitro study of the pH-lowering potential of salivary lactobacilli associated with dental caries. J Appl Microbiol 90(6): 1015-1018.
15. McLean JS, Fansler SJ, Majors PD, McAteer K, Allen LZ, Shirtliff ME, Lux R, Shi W (2012) Identifying low pH active and lactate-utilizing taxa within oral microbiome communities from healthy children using stable isotope probing techniques. PLoS One 7(3): e32219. doi: 10. 1371/journal. pone. 0032219.
16. Hojo S, Komatsu M, Okuda R, Takahashi N, Yamada T (1994) Acid profiles and pH of carious dentin in active and arrested lesions. J Dent Res 73(12): 1853-1857.
17. Byun R, Nadkarni MA, Chhour KL, Martin FE, Jacques NA, Hunter N (2004) Quantitative analysis of diverse Lactobacillus species present in advanced dental caries. J Clin Microbiol 42(7): 3128-3136. doi: 10. 1128/JCM. 42. 7. 3128-3136. 2004.
18. Martin FE, Nadkarni MA, Jacques NA, Hunter N (2002) Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. J Clin Microbiol 40(5): 1698-1704.
19. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2. 0. Bioinformatics 23(21): 2947-2948. doi: 10. 1093/bioinformatics/btm404.
20. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215(3): 403-410. doi: 10. 1016/S0022-2836(05)80360-2.
21. Nadkarni MA, Martin FE, Jacques NA, Hunter N (2002) Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology 148(Pt 1): 257-266.
22. Smith CJ, Osborn AM (2009) Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol 67(1): 6-20. doi: 10. 1111/j. 1574-6941. 2008. 00629. x.
23. Nadkarni MA, Martin FE, Hunter N, Jacques NA (2009) Methods for optimizing DNA extraction before quantifying oral bacterial numbers by real-time PCR. FEMS Microbiol Lett 296(1): 45-51. doi: 10. 1111/j. 1574-6968. 2009. 01629. x.
24. Lee ZM, Bussema C, 3rd, Schmidt TM (2009) rrnDB: documenting the number of rRNA and tRNA genes in bacteria and archaea. Nucleic Acids Res 37 (Database issue): D489-493. doi: 10. 1093/nar/gkn689.
25. Holm S (1979) A simple sequentially rejective multiple test procedure. Scand J Statist 6: 65-70.
26. Schachtele CF, Jensen ME (1982) Comparison of methods for monitoring changes in the pH of human dental plaque. J Dent Res 61(10): 1117-1125.
27. Kleinberg I, Jenkins GN, Chatterjee R, Wijeyeweera L (1982) The antimony pH electrode and its role in the assessment and interpretation of dental plaque pH. J Dent Res 61(10): 1139-1147.
28. Stephen RM (1940) Changes in hydrogen ion concentration on tooth surfaces and in carious lesions. J Am Dent Assoc 27: 718-723.
29. Bentley KD, Haldi J, Law ML, Ramsey DA, Wynn W (1956) Dental caries in relation to pH on tooth surfaces. I. pH and lactate concentration in relation to the extent of the lesions in rats' teeth. J Nutr 60(3): 427-435.
30. Igarashi K, Kamiyama K, Yamada T (1981) Measurement of pH in human dental plaque in vivo with an ion-sensitive transistor electrode. Archives of oral biology 26(3): 203-207.
31. Hassell TM (1971) Construction of micro-antimony electrodes for use in radio telemetry of plaque pH. Helvetica odontologica acta 15(1): 50-51.
32. Tahmassebi JF, Duggal MS (1997) The effect of different methods of drinking on the pH of dental plaque in vivo. Intern J Paediatr Dent Br Paedodontic Soc Intern Assoc Dent Children 7(4): 249-254.
33. Roos EH, Donly KJ (2002) In vivo dental plaque pH variation with regular and diet soft drinks. Pediatr Dent 24(4): 350-353.
34. Jawale BA, Bendgude V, Mahuli AV, Dave B, Kulkarni H, Mittal S (2012) Dental plaque pH variation with regular soft drink, diet soft drink, and high energy drink: an in vivo study. J Contemp Dent Pract 13(2): 201-204.
35. Dirksen TR, Little MF, Bibby BG (1963) The pH of carious cavities-II. The pH at different depths in isolated cavities. Archives of oral biology 8: 91-97.
36. Hojo S, Takahashi N, Yamada T (1991) Acid profile in carious dentin. J Dent Res 70(3): 182-186.
37. Hopkins MJ, Englyst HN, Macfarlane S, Furrie E, Macfarlane GT, McBain AJ (2003) Degradation of cross-linked and non-cross-linked arabinoxylans by the intestinal microbiota in children. Appl Environ Microbiol 69(11): 6354-6360.
38. Jansen HJ, van der Hoeven JS (1997) Protein degradation by Prevotella intermedia and Actinomyces meyeri supports the growth of nonprotein-cleaving oral bacteria in serum. J Clin Periodontol 24(5): 346-353.
39. Takahashi N (2003) Acid-neutralizing activity during amino acid fermentation by Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum. Oral Microbiol Immunol 18(2): 109-113.
40. Bradshaw DJ, Marsh PD (1998) Analysis of pH-driven disruption of oral microbial communities in vitro. Caries Res 32(6): 456-462.
41. Periasamy S, Kolenbrander PE (2010) Central role of the early colonizer Veillonella sp. in establishing multispecies biofilm communities with initial, middle, and late colonizers of enamel. J Bacteriol 192(12): 2965-2972. doi: 10. 1128/JB. 01631-09.
42. Chalmers NI, Palmer RJ Jr, Cisar JO, Kolenbrander PE (2008) Characterization of a Streptococcus sp.-Veillonella sp. community micromanipulated from dental plaque. J Bacteriol 190(24): 8145-8154. doi: 10. 1128/JB. 00983-08.
43. Horiuchi M, Washio J, Mayanagi H, Takahashi N (2009) Transient acid-impairment of growth ability of oral Streptococcus, Actinomyces, and Lactobacillus: a possible ecological determinant in dental plaque. Oral Microbiol Immunol 24(4): 319-324. doi: 10. 1111/j. 1399-302X. 2009. 00517. x.
44. Berkovitz BKB, Holland GR, Moxham BJ (2002) Oral anatomy, histology and embryology. 3rd edn. Mosby.
45. Vanuspong W, Eisenburger M, Addy M (2002) Cervical tooth wear and sensitivity: erosion, softening, and rehardening of dentine: effects of pH, time, and ultrasonication. J Clin Periodontol 29(4): 351-357.
46. Marsh PD (2003) Are dental diseases examples of ecological catastrophes? Microbiology 149(Pt 2): 279-294.
47. Bradshaw DJ, Marsh PD, Allison C, Schilling KM (1996) Effect of oxygen, inoculum composition, and flow rate on development of mixed-culture oral biofilms. Microbiology 142(Pt 3): 623-629.
48. Munson MA, Banerjee A, Watson TF, Wade WG (2004) Molecular analysis of the microflora associated with dental caries. J Clin Microbiol 42(7): 3023-3029. doi: 10. 1128/JCM. 42. 7. 3023-3029. 2004.
49. van Houte J, Lopman J, Kent R (1994) The predominant cultivable flora of sound and carious human root surfaces. J Dent Res 73(11): 1727-1734.
50. Sansone C, van Houte J, Joshipura K, Kent R, Margolis HC (1993) The association of mutans streptococci and non-mutans streptococci capable of acidogenesis at a low pH with dental caries on enamel and root surfaces. J Dent Res 72(2): 508-516.
51. Loesche WJ (1986) Role of Streptococcus mutans in human dental decay. Microbiol Rev 50(4): 353-380.
52. Gross EL, Beall CJ, Kutsch SR, Firestone ND, Leys EJ, Griffen AL (2012) Beyond Streptococcus mutans: dental caries onset linked to multiple species by 16S rRNA community analysis. PLoS One 7(10): e47722. doi: 10. 1371/journal. pone. 0047722.
53. Aas JA, Griffen AL, Dardis SR, Lee AM, Olsen I, Dewhirst FE, Leys EJ, Paster BJ (2008) Bacteria of dental caries in primary and permanent teeth in children and young adults. J Clin Microbiol 46(4): 1407-1417. doi: 10. 1128/JCM. 01410-07.
54. Gross EL, Leys EJ, Gasparovich SR, Firestone ND, Schwartzbaum JA, Janies DA, Asnani K, Griffen AL (2010) Bacterial 16S sequence analysis of severe caries in young permanent teeth. J Clin Microbiol 48(11): 4121-4128. doi: 10. 1128/JCM. 01232-10.
55. Jenkinson HF (2011) Beyond the oral microbiome. Environ Microbiol 13(12): 3077-3087. doi: 10. 1111/j. 1462-2920. 2011. 02573. x.
56. Klein D (2002) Quantification using real-time PCR technology: applications and limitations. Trends Mol Med 8(6): 257-260.
57. Smith CJ, Nedwell DB, Dong LF, Osborn AM (2006) Evaluation of quantitative polymerase chain reaction-based approaches for determining gene copy and gene transcript numbers in environmental samples. Environ Microbiol 8(5): 804-815. doi: 10. 1111/j. 1462-2920. 2005. 00963. x.
58. Love JL, Scholes P, Gilpin B, Savill M, Lin S, Samuel L (2006) Evaluation of uncertainty in quantitative real-time PCR. J Microbiol Methods 67(2): 349-356. doi: 10. 1016/j. mimet. 2006. 04. 005.
59. Frencken JE, Leal SC, Navarro MF (2012) Twenty-five-year atraumatic restorative treatment (ART) approach: a comprehensive overview. Clinical oral investigations 16(5): 1337-1346. doi: 10. 1007/s00784-012-0783-4.
60. Topaloglu-Ak A, Eden E, Frencken JE, Oncag O (2009) Two-year survival rate of class II composite resin restorations prepared by ART with and without a chemomechanical caries removal gel in primary molars. Clinical oral investigations 13(3): 325-332. doi: 10. 1007/s00784-008-0241-5.
61. Eden E, Topaloglu-Ak A, Frencken JE, van't Hof M (2006) Survival of self-etch adhesive Class II composite restorations using ART and conventional cavity preparations in primary molars. Am J Dent 19(6): 359-363.
62. Louw AJ, Sarvan I, Chikte UM, Honkala E (2002) One-year evaluation of atraumatic restorative treatment and minimum intervention techniques on primary teeth. SADJ: J S Afr Dental Assoc = tydskrif van die Suid-Afrikaanse Tandheelkundige Vereniging 57(9): 366-371.
63. Cefaly DF, Barata TJ, Bresciani E, Fagundes TC, Lauris JR, Navarro MF (2007) Clinical evaluation of multiple-surface ART restorations: 12-month follow-up. J Dent Child (Chic) 74(3): 203-208.
64. Faccin ES, Ferreira SH, Kramer PF, Ardenghi TM, Feldens CA (2009) Clinical performance of ART restorations in primary teeth: a survival analysis. J Clin Pediatr Dent 33(4): 295-298.
65. Taifour D, Frencken JE, Beiruti N, van 't Hof MA, Truin GJ (2002) Effectiveness of glass-ionomer (ART) and amalgam restorations in the deciduous dentition: results after 3 years. Caries Res 36 (6): 437-444. doi: 66531.
66. Beiruti N, Frencken JE, van't Hof MA, Taifour D, van Palenstein Helderman WH (2006) Caries-preventive effect of a one-time application of composite resin and glass ionomer sealants after 5 years. Caries Res 40(1): 52-59. doi: 10. 1159/000088907.
67. Zanata RL, Fagundes TC, Freitas MC, Lauris JR, Navarro MF (2011) Ten-year survival of ART restorations in permanent posterior teeth. Clin Oral Inv 15(2): 265-271. doi: 10. 1007/s00784-009-0378-x.
68. Prentice LH, Tyas M, Burrow MF (2006) The effect of oxalic acid incorporation on the setting time and strength of glass-ionomer cement. Acta biomaterialia 2(1): 109-112. doi: 10. 1016/j. actbio. 2005. 08. 007.
69. McInnes-Ledoux PM, Weinberg R, Grogono A (1989) Bonding glass-ionomer cements to chemomechanically-prepared dentin. Dent Mater Off Publ Acad Dent Mater 5(3): 189-193.