Zebrafish models for assessing developmental and reproductive toxicity

Neurotoxicology and Teratology

Volumn 42, Issue , 2014, Pages 35-42

  He, Jian Hui ^a,b   Gao, Ji Min ^a   Huang, Chang Jiang ^a   Li, Chun Qi ^a,b  

^a WENZHOU MEDICAL UNIVERSITY   (China)

^b Transfarland   (China)

Author keywords

Chemical toxicity; Developmental toxicity; Embryo toxicity; Reproductive toxicity; Zebrafish

Indexed keywords

TERATOGENIC AGENT;

ANIMAL; ANIMAL BEHAVIOR; ANIMAL MODEL; DRUG EFFECTS; DRUG SCREENING; EMBRYOLOGY; GROWTH, DEVELOPMENT AND AGING; LARVA; PHYSIOLOGY; PROCEDURES; REPRODUCTION; TERATOLOGY; TOXICITY; ZEBRA FISH;

ANIMALS; BEHAVIOR, ANIMAL; DRUG EVALUATION, PRECLINICAL; LARVA; MODELS, ANIMAL; REPRODUCTION; TERATOGENS; TERATOLOGY; ZEBRAFISH;

EID: 84894231554     PISSN: 08920362     EISSN: 18729738     Source Type: Journal    
DOI: 10.1016/j.ntt.2014.01.006     Document Type: Review

References (114)

1. Airhart M.J., Lee D.H., Wilson T.D., Miller B.E., Miller M.N., Skalko R.G. Movement disorders and neurochemical changes in zebrafish larvae after bath exposure to fluoxetine (PROZAC). Neurotoxicol Teratol 2007, 29:652-664.
2. Akimenko M.A., Ekker M. Anterior duplication of the Sonic hedgehog expression pattern in the pectoral fin buds of zebrafish treated with retinoic acid. Dev Biol 1995, 170:243-247.
3. Ali S., Champagne D.L., Spaink H.P., Richardson M.K. Zebrafish embryos and larvae: a new generation of disease models and drug screens. Birth Defects Res C Embryo Today 2011, 93(2):115-133.
4. Bailey J., Oliveri A., Levin E.D. Zebrafish model systems for developmental neurobehavioral toxicology. Birth Defects Res C Embryo Today 2013, 99(1):14-23.
5. Baraban S.C., Taylor M.R., Castro P.A., Baier H. Pentylenetetrazole induced changes in zebrafish behavior, neural activity and c-fos expression. Neuroscience 2005, 131:759-768.
6. Berghmans S., Butler P., Goldsmith P., Waldron G., Gardner I., Golder Z., et al. Zebrafish based assays for the assessment of cardiac, visual and gut function-potential safety screens for early drug discovery. J Pharmacol Toxicol Methods 2008, 58:59-68.
7. Boehmler W., Carr T., Thisse C., Thisse B., Canfield V.A., Levenson R. D4 Dopamine receptor genes of zebrafish and effects of the antipsychotic clozapine on larval swimming behaviour. Genes Brain Behav 2007, 6:155-166.
8. Borski R.J., Hodson R.G. Fish research and the institutional animal care and use committee. ILAR J 2003, 44(4):286-294.
9. Boverhof D.R., Zacharewski T.R. Toxicogenomics in risk assessment: applications and needs. Toxicol Sci 2006, 89(2):352-360.
10. 50 test in chemical assessment: the fish embryo toxicity test goes multi-species-an update. ALTEX 2005, 22:87-102.
11. Brion F., Nilsen B.M., Eidem J.K., Goksøyr A., Porcher J.M. Development and validation of an enzyme-linked immunosorbent assay to measure vitellogenin in the zebrafish (Danio rerio). Environ Toxicol Chem 2002, 21(8):1699-1708.
12. Brion F., Tyler C.R., Palazzi X., Laillet B., Porcher J.M., Garric J., et al. Impacts of 17beta-estradiol, including environmentally relevant concentrations, on reproduction after exposure during embryo-larval-, juvenile- and adult-life stages in zebrafish (Danio rerio). Aquat Toxicol 2004, 68(3):193-217.
13. Bull J., Levin B. Perspectives: microbiology. Mice are not furry Petri dishes. Science 2000, 287:1409-1410.
14. Buss R.R., Drapeau P. Synaptic drive to motoneurons during fictive swimming in the developing zebrafish. J Neurophysiol 2001, 86:197-210.
15. Chakraborty C., Hsu C.H., Wen Z.H., Lin C.S., Agoramoorthy G. Zebrafish: a complete animal model for in vivo drug discovery and development. Curr Drug Metab 2009, 10(2):116-124.
16. Chen J., Huang C., Zheng L., Simonich M., Bai C., Tanguay R., et al. Trimethyltin chloride (TMT) neurobehavioral toxicity in embryonic zebrafish. Neurotoxicol Teratol 2011, 33(6):721-726.
17. Chen J., Chen Y., Liu W., Bai C., Liu X., Liu K., et al. Developmental lead acetate exposure induces embryonic toxicity and memory deficit in adult zebrafish. Neurotoxicol Teratol 2012, 34(6):581-586.
18. Chen X., Huang C., Wang X., Chen J., Bai C., Chen Y., et al. BDE-47 disrupts axonal growth and motor behavior in developing zebrafish. Aquat Toxicol 2012, 120-121:35-44.
19. Combes R., Barratt M., Balls M. An overall strategy for the testing of chemicals for human hazard and risk assessment under the EU REACH system. Altern Lab Anim 2003, 31:7-19.
20. de Esch C., Slieker R., Wolterbeek A., Woutersen R., de Groot D. Zebrafish as potential model for developmental neurotoxicity testing: a mini review. Neurotoxicol Teratol 2012, 34:545-553.
21. Delvecchio C., Tiefenbach J., Krause H.M. The zebrafish: a powerful platform for in vivo, HTS drug discovery. Assay Drug Dev Technol 2011, 9(4):354-361.
22. Deng J., Liu C., Yu L., Zhou B. Chronic exposure to environmental levels of tribromophenol impairs zebrafish reproduction. Toxicol Appl Pharmacol 2010, 243(1):87-95.
23. Dong W., Teraoka H., Kondo S., Hiraga T. 2,3,7,8-Tetrachlorodibenzo-p-dioxin induces apoptosis in the dorsal midbrain of zebrafish embryos by activation of arylhydrocarbon receptor. Neurosci Lett 2001, 303:169-172.
24. Du Y., Shi X., Liu C., Yu K., Zhou B. Chronic effects of water-borne PFOS exposure on growth, survival and hepatotoxicity in zebrafish: a partial life-cycle test. Chemosphere 2009, 74(5):723-729.
25. Ducharme N.A., Peterson L.E., Benfenati E., Reif D., McCollum C.W., Gustafsson J.Å., et al. Meta-analysis of toxicity and teratogenicity of 133 chemicals from zebrafish developmental toxicity studies. Reprod Toxicol 2013, 41:98-108.
26. EC (European Commission) White Paper-strategy for a future chemicals policy 2001, European Commission, Brussels.
27. EPA OPPTS 850.1075: fish acute toxicity test, freshwater and marine. Prevention, pesticides and toxic substances 1996, United States Environmental Protection Agency, [7101].
28. EPA OPPTS 850.1400 fish early-life stage toxicity test. Prevention, pesticides and toxic substances 1996, United States Environmental Protection Agency, [7101].
29. Fenske M., Maack G., Schäfers C., Segner H. An environmentally relevant concentration of estrogen induces arrest of male gonad development in zebrafish, Danio rerio. Environ Toxicol Chem 2005, 24(5):1088-1098.
30. Fleming A. Zebrafish as an alternative model organism for disease modelling and drug discovery: implications for the 3Rs 2007, NC3Rs: National Centre for the Replacement, Refinement and Reduction of Animals in research.
31. Fort D.J., Dawson D.A., Bantle J.A. Development of a metabolic activation system for the frog embryo teratogenesis assay: Xenopus (FETAX). Teratog Carcinog Mutagen 1988, 8:251-263.
32. Genschow E., Spielmann H., Scholz G., Seiler A., Brown N., Piersma A., et al. The ECVAM international validation study on in vitro embryotoxicity tests: results of the definitive phase and evaluation of prediction models. European Centre for the Validation of Alternative Methods. Altern Lab Anim 2002, 30(2):151-176.
33. Gerlai R., Ahmad F., Prajapati S. Differences in acute alcohol-induced behavioral responses among zebrafish populations. Alcohol Clin Exp Res 2008, 32:1763-1773.
34. Granato M., van Eeden F.J., Schach U., Trowe T., Brand M., Furutani-Seiki M., et al. Genes controlling and mediating locomotion behavior of the zebrafish embryo and larva. Development 1996, 123:399-413.
35. Gustafson A.L., Stedman D.B., Ball J., Hillegass J.M., Flood A., Zhang C.X., et al. Inter-laboratory assessment of a harmonized zebrafish developmental toxicology assay - progress report on phase I. Reprod Toxicol 2012, 33(2):155-164.
36. Haldi M., Ton C., Seng W.L., McGrath P. Human melanoma cells transplanted into zebrafish proliferate, migrate, produce melanin, form masses and stimulate angiogenesis in zebrafish. Angiogenesis 2006, 9:139-151.
37. Haldi M., Harden M., D' Amico L., Delise A., Seng W.L. Developmental toxicity assessment in zebrafish. Zebrafish methods for assessing drug safety and toxicity 2011, 15-25. John Wiley & Sons, Inc., New Jersey. P. McGrath (Ed.).
38. Hallare A., Nagel K., Köhler H.R., Triebskorn R. Comparative embryotoxicity and proteotoxicity of three carrier solvents to zebrafish (Danio rerio) embryos. Ecotoxicol Environ Saf 2006, 63:378-388.
39. He J., Yang D., Wang C., Liu W., Liao J., Xu T., et al. Chronic zebrafish low dose decabrominated diphenyl ether (BDE-209) exposure affected parental gonad development and locomotion in F1 offspring. Ecotoxicology 2011, 20(8):1813-1822.
40. He J., Guo S., Zhu F., Zhu J., Chen Y., Huang C., et al. A zebrafish phenotypic assay for assessing drug-induced hepatotoxicity. J Pharmacol Toxicol Methods 2013, 67(1):25-32.
41. Heiden T.K., Hutz R.J., Carvan M.J. Accumulation, tissue distribution, and maternal transfer of dietary 2,3,7,8,-tetrachlorodibenzo-p-dioxin: impacts on reproductive success of zebrafish. Toxicol Sci 2005, 87(2):497-507.
42. Hill A., Mesens N., Steemans M., Xu J.J., Aleo M.D. Comparisons between in vitro whole cell imaging and in vivo zebrafish-based approaches for identifying potential human hepatotoxicants earlier in pharmaceutical development. Drug Metab Rev 2012, 44(1):127-140.
43. Huang H., Huang C., Wang L., Ye X., Bai C., Simonich M.T., et al. Toxicity, uptake kinetics and behavior assessment in zebrafish embryos following exposure to perfluorooctanesulphonicacid (PFOS). Aquat Toxicol 2010, 98:139-147.
44. Incardona J.P., Collier T.K., Scholz N.L. Defects in cardiac function precede morphological abnormalities in fish embryos exposed to polycyclic aromatic hydrocarbons. Toxicol Appl Pharmacol 2004, 196:191-205.
45. Irons T.D., MacPhail R.C., Hunter D.L., Padilla S. Acute neuroactive drug exposures alter locomotor activity in larval zebrafish. Neurotoxicol Teratol 2009, 32:84-90.
46. ISO Water quality-determination of the acute toxicity of waste water to zebrafish eggs (Danio rerio). ISO 15088:2007 2007.
47. Jin M., Zhang X., Wang L., Huang C., Zhang Y., Zhao M. Developmental toxicity of bifenthrin in embryo-larval stages of zebrafish. Aquat Toxicol 2009, 95(4):347-354.
48. Jin Y., Zheng S., Fu Z. Embryonic exposure to cypermethrin induces apoptosis and immunotoxicity in zebrafish (Danio rerio). Fish Shellfish Immunol 2011, 30:1049-1054.
49. Jing R., Huang C., Bai C., Tanguay R.L., Dong Q. Optimization of activation, collection, dilution, and storage methods for zebrafish sperm. Aquaculture 2009, 290:165-171.
50. Kimmel C.B., Ballard W.W., Kimmel S.R., Ullmann B., Schilling T.F. Stages of embryonic development of the zebrafish. Dev Dyn 1995, 203(3):253-310.
51. Koc N.D., Muslu M.N. The histological examination of Mus musculus' stomach which was exposed to hunger and thirst stress: a study with light microscope. Pak J Biol Sci 2007, 10(17):2988-2991.
52. Kola I., Landis J. Can the pharmaceutical industry reduce attrition rates?. Nat Rev Drug Discov 2004, 3(8):711-715.
53. Kuriyama S., Talsness C., Grote K., Chahoud I. Developmental exposure to low dose PBDE 99: effects on male fertility and neurobehavior in rat offspring. Environ Health Perspect 2005, 113:149-154.
54. Lammer E., Carr G.J., Wendler K., Rawlings J.M., Belanger S.E., Braunbeck T. Is the fish embryo toxicity test (FET) with the zebrafish (Danio rerio) a potential alternative for the fish acute toxicity test?. Comp Biochem Physiol C Toxicol Pharmacol 2009, 149(2):196-209.
55. Leal M.C., Cardoso E.R., Nóbrega R.H., Batlouni S.R., Bogerd J., França L.R., et al. Histological and stereological evaluation of zebrafish (Danio rerio) spermatogenesis with an emphasis on spermatogonial generations. Biol Reprod 2009, 81(1):177-187.
56. Levin E.D., Swain H.A., Donerly S., Linney E. Developmental chlorpyrifos effects on hatchling zebrafish swimming behavior. Neurotoxicol Teratol 2004, 26:719-723.
57. Levin E.D., Aschner M., Heberlein U., Ruden D., Welsh-Bohmer K.A., et al. Genetic aspects of behavioral neurotoxicology. Neurotoxicology 2009, 30(5):741-753.
58. Lewis K.E., Eisen J.S. From cells to circuits: development of the zebrafish spinal cord. Prog Neurobiol 2003, 69:419-449.
59. Li L., Dowling J.E. A dominant form of inherited retinal degeneration caused by a non-photoreceptor cell-specific mutation. Proc Natl Acad Sci U S A 1997, 94:11645-11650.
60. Lieschke G.J., Currie P.D. Animal models of human disease: zebrafish swim into view. Nat Rev Genet 2007, 8:353-367.
61. Lilienblum W., Dekant W., Foth H., Gebel T., Hengstler J.G., Kahl R., et al. Alternative methods to safety studies in experimental animals: role in the risk assessment of chemicals under the new European Chemicals Legislation (REACH). Arch Toxicol 2008, 82:211-236.
62. Lilienthal H., Hack A., Roth-Härer A., Grande S.W., Talsness C.E. Effects of developmental exposure to 2,2,4,4,5-pentabromodiphenyl ether (PBDE-99) on sex steroids, sexual development, and sexually dimorphic behavior in rats. Environ Health Perspect 2006, 114(2):194-201.
63. Liu C., Yu L., Deng J., Lam P.K., Wu R.S., Zhou B. Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish. Aquat Toxicol 2009, 93(2-3):131-137.
64. Liu C., Zhang X., Deng J., Hecker M., Al-Khedhairy A., Giesy J.P., et al. Effects of prochloraz or propylthiouracil on the cross-talk between the HPG, HPA, and HPT axes in zebrafish. Environ Sci Technol 2011, 45(2):769-775.
65. Lühe A., Suter L., Ruepp S., Singer T., Weiser T., Albertini S. Toxicogenomics in the pharmaceutical industry: hollow promises or real benefit?. Mutat Res 2005, 575(1-2):102-115.
66. Makri A., Goveia M., Balbus J., Parkin R. Children's susceptibility to chemicals: a review by developmental stage. J Toxicol Environ Health B Crit Rev 2004, 7(6):417-435.
67. Marathe M., Thomas G. Current status of animal testing in reproductive toxicology. India J Pharmacol 1990, 22:192-201.
68. McCollum C.W., Ducharme N.A., Bondesson M., Gustafsson J.A. Developmental toxicity screening in zebrafish. Birth Defects Res C Embryo Today 2011, 93(2):67-114.
69. McGrath P., Li C. Zebrafish: a predictive model for assessing drug-induced toxicity. Drug Discov Today 2008, 13(9-10):394-401.
70. Moens C.B., Prince V.E. Constructing the hindbrain: insights from the zebrafish. Dev Dyn 2002, 224:1-17.
71. Oberemm A. The use of a refined zebrafish embryo bioassay for the assessment of aquatic toxicity. Lab Anim 2000, 29:32-40.
72. OECD OECD guidelines for the testing of chemicals. Section2: effects on biotic systems test no. 203: acute toxicity for fish 1992, Organization for Economic Cooperation and Development, Paris, France.
73. OECD OECD guidelines for the testing of chemicals. Section2: effects on biotic systems test no. 210: fish, early-life stage toxicity test 1992, Organization for Economic Cooperation and Development, Paris, France.
74. OECD OECD guidelines for the testing of chemicals. Section2: effects on biotic systems test no. 212: fish, short-term toxicity test on embryo and sac-fry stages 1998, Organization for Economic Cooperation and Development, Paris, France.
75. OECD OECD Environment, Health and Safety Publications Series on Testing and Assessment No. 23. Guidance document on aquatic toxicity testing of difficult substances and mixtures. Environment Directorate 2000, OECD, Paris.
76. Paquet D., Bhat R., Sydow A., Mandelkow E.M., Berg S., Hellberg S., et al. A zebrafish model of tauopathy allows in vivo imaging of neuronal cell death and drug evaluation. J Clin Invest 2009, 119(5):1382-1395.
77. Parng C. In vivo zebrafish assays for toxicity testing. Curr Opin Drug Discov Devel 2005, 8:100-106.
78. Parng C., Seng W.L., Semino C., McGrath P. Zebrafish: a preclinical model for drug screening. Assay Drug Dev Technol 2002, 1:41-48.
79. Parng C., Anderson N., Ton C., McGrath P. Zebrafish apoptosis assays for drug discovery. Methods Cell Biol 2004, 76:75-85.
80. Perkins E.J., Ankley G.T., Crofton K.M., Garcia-Reyero N., LaLone C.A., Johnson M.S., et al. Current perspectives on the use of alternative species in human health and ecological hazard assessments. Environ Health Perspect 2013, 121(9):1002-1010.
81. Pugsley M.K., Gallacher D.J., Towart R., Authier S., Curtis M.J. Methods in safety pharmacology in focus. J Pharmacol Toxicol Methods 2008, 58(2):69-71.
82. Redfern W.S., Waldron G., Winter M.J., Butler P., Holbrook M., Wallis R., et al. Zebrafish assays as early safety pharmacology screens: paradigm shift or red herring?. J Pharmacol Toxicol Methods 2008, 58:110-117.
83. Reimers M.J., Hahn M.E., Tanguay R.L. Two zebrafish alcohol dehydrogenases share common ancestry with mammalian class I, II, IV, and V alcohol dehydrogenase genes but have distinct functional characteristics. J Biol Chem 2004, 279:38303-38312.
84. Renier C., Faraco J.H., Bourgin P., Motley T., Bonaventure P., Rosa F., et al. Genomic and functional conservation of sedative-hypnotic targets in the zebrafish. Pharmacogenet Genomics 2007, 17:237-253.
85. Roberts A. Early functional organization of spinal neurons in developing lower vertebrates. Brain Res Bull 2000, 53:585-593.
86. Rubinstein A.L. Zebrafish assays for drug toxicity screening. Expert Opin Drug Metab Toxicol 2006, 2(2):231-240.
87. Saint-Amant L., Drapeau P. Time course of the development of motor behaviors in the zebrafish embryo. J Neurobiol 1998, 37:622-632.
88. Samson J.C., Goodridge R., Olobatuyi F., Weis J.S. Delayed effects of embryonic exposure of zebrafish (Danio rerio) to methylmercury (MeHg). Aquat Toxicol 2001, 51:369-376.
89. Saverino C., Gerlai R. The social zebrafish: behavioral responses to conspecific, heterospecific, and computer animated fish. Behav Brain Res 2008, 191:77-87.
90. Scheil V., Zurn A., Kohler H.R., Triebskorn R. Embryo development, stress protein (Hsp70) responses, and histopathology in zebrafish (Danio rerio) following exposure to nickel chloride, chlorpyrifos, and binary mixtures of them. Environ Toxicol 2010, 25:83-93.
91. Schnorr S.J., Steenbergen P.J., Richardson M.K., Champagne D.L. Measuring thigmotaxis in larval zebrafish. Behav Brain Res 2012, 228:367-374.
92. Selderslaghs I.W., Van Rompay A.R., De Coen W., Witters H.E. Development of a screening assay to identify teratogenic and embryotoxic chemicals using the zebrafish embryo. Reprod Toxicol 2009, 28(3):308-320.
93. Selderslaghs I.W., Hooyberghs J., De Coen W., Witters H.E. Locomotor activity in zebrafish embryos: a new method to assess developmental neurotoxicity. Neurotoxicol Teratol 2010, 32(4):460-471.
94. Shi X., Liu C., Wu G., Zhou B. Waterborne exposure to PFOS causes disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae. Chemosphere 2009, 77(7):1010-1018.
95. Sipes N.S., Padilla S., Knudsen T.B. Zebrafish: as an integrative model for twenty-first century toxicity testing. Birth Defects Res C Embryo Today 2011, 93(3):256-267.
96. Sison M., Gerlai R. Associative learning in zebrafish (Danio rerio) in the plus maze. Behav Brain Res 2010, 207:99-104.
97. Skauli K.S., Reitan J.B., Walther B.T. Hatching in zebrafish (Danio rerio) embryos exposed to a 50Hz magnetic field. Bioelectromagnetics 2000, 21:407-410.
98. Slotkin T.A., Seidler F.J. Developmental exposure to terbutaline and chlorpyrifos, separately or sequentially, elicits presynaptic serotonergic hyperactivity in juvenile and adolescent rats. Brain Res Bull 2007, 73(4-6):301-309.
99. Spitsbergen J.M., Kent M.L. The state of the art of the zebrafish model for toxicology and toxicologic pathology research - advantages and current limitations. Toxicol Pathol 2003, 31:62-87. [Suppl.].
100. Stoker T.E., Cooper R.L., Lambright C.S., Wilson V.S., Furr J., Gray L.E. In vivo and in vitro anti-androgenic effects of DE-71, a commercial polybrominated diphenyl ether (PBDE) mixture. Toxicol Appl Pharmacol 2005, 207:78-88.
101. Ton C., Parng C. The use of zebrafish for assessing ototoxic and otoprotective agents. Hear Res 2005, 208:79-88.
102. Ton C., Lin Y., Willett C. Zebrafish as a model for developmental neurotoxicity testing. Birth Defects Res A Clin Mol Teratol 2006, 76:553-567.
103. Tseng L.H., Lee C.W., Pan M.H., Tsai S.S., Li M.H., Chen J.R., et al. Postnatal exposure of the male mouse to 2,2',3,3',4,4',5,5',6,6'-decabrominated diphenyl ether: decreased epididymal sperm functions without alterations in DNA content and histology in testis. Toxicology 2006, 224:33-43.
104. Valente A., Huang K.H., Portugues R., Engert F. Ontogeny of classical and operant learning behaviors in zebrafish. Learn Mem 2012, 19:170-177.
105. Van den Belt K., Verheyen R., Witters H. Reproductive effects of ethynylestradiol and 4t-octylphenol on the zebrafish (Danio rerio). Arch Environ Contam Toxicol 2001, 41(4):458-467.
106. Versonnen B.J., Roose P., Monteyne E.M., Janssen C.R. Estrogenic and toxic effects of methoxychlor on zebrafish (Danio rerio). Environ Toxicol Chem 2004, 23:2194-2201.
107. Wang X., Wang F., Wu X., Zhao Z., Liu J., Huang C., et al. The use of cryomicroscopy in guppy sperm freezing. Cryobiology 2010, 61:182-188.
108. Wang R.L., Bencic D., Lazorchak J., Villeneuve D., Ankley G.T. Transcriptional regulatory dynamics of the hypothalamic-pituitary-gonadal axis and its peripheral pathways as impacted by the 3-beta HSD inhibitor trilostane in zebrafish (Danio rerio). Ecotoxicol Environ Saf 2011, 74(6):1461-1470.
109. Weigt S., Huebler N., Strecker R., Braunbeck T., Broschard T.H. Zebrafish (Danio rerio) embryos as a model for testing proteratogens. Toxicology 2011, 281(1-3):25-36.
110. Willey J.B., Krone P.H. Effects of endosulfan and nonylphenol on the primordial germ cell population in pre-larval zebrafish embryos. Aquat Toxicol 2001, 54:113-123.
111. Wilson S.W., Brand M., Eisen J.S. Patterning the zebrafish central nervous system. Results Probl Cell Differ 2002, 40:181-215.
112. Yang L., Ho N.Y., Alshut R., Legradi J., Weiss C., Reischl M., et al. Zebrafish embryos as models for embryotoxic and teratological effects of chemicals. Reprod Toxicol 2009, 28(2):245-253.
113. Zhu J., Xu Y., He J., Yu H., Huang C., Gao J., et al. Human cardiotoxic drugs delivered by soaking and microinjection induce cardiovascular toxicity in zebrafish. J Appl Toxicol 2013, 10.1002/jat.2843.
114. Zon L.I., Peterson R.T. In vivo drug discovery in the zebrafish. Nat Rev Drug Discov 2005, 4(1):35-44.