Production of scFv-conjugated affinity silk film and its application to a novel enzyme-linked immunosorbent assay

Scientific Reports

Volumn 4, Issue , 2014, Pages

  Sato, Mitsuru ^a   Kojima, Katsura ^a   Sakuma, Chisato ^a   Murakami, Maria ^a   Tamada, Yasushi ^a   Kitani, Hiroshi ^a  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BROMIDE; HYBRID PROTEIN; IMMOBILIZED ANTIBODY; LITHIUM BROMIDE; LITHIUM DERIVATIVE; SILK; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; WISKOTT ALDRICH SYNDROME PROTEIN;

ANIMAL; BIOSYNTHESIS; BOMBYX; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; IMMUNOLOGY; IMMUNOPRECIPITATION; METABOLISM;

ANIMALS; ANTIBODIES, IMMOBILIZED; BOMBYX; BROMIDES; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOPRECIPITATION; LITHIUM COMPOUNDS; RECOMBINANT FUSION PROTEINS; SILK; SINGLE-CHAIN ANTIBODIES; WISKOTT-ALDRICH SYNDROME PROTEIN;

EID: 84894172969     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep04080     Document Type: Article

References (30)

1. Rockwood, D. N. et al. Materials fabrication from Bombyx mori silk fibroin. Nat. Protocols 6, 1612-1631 (2011).
2. Kim, U. J. et al. Structure and properties of silk hydrogels. Biomacromolecules 5, 786-792 (2004).
3. Nazarov, R., Jin, H. J. & Kaplan, D. L. Porous 3-D scaffolds from regenerated silk fibroin. Biomacromolecules 5, 718-726 (2004). (Pubitemid 38702237)
4. Tamada, Y. New process to form a silk fibroin porous 3-D structure. Biomacromolecules 6, 3100-3106 (2005). (Pubitemid 41764068)
5. Gotoh, Y., Tsukada, M., Minoura, N. & Imai, Y. Synthesis of poly (ethylene glycol) - silk fibroin conjugates and surface interaction between L929 cells and the conjugates. Biomaterials 18, 267-271 (1997). (Pubitemid 27064803)
6. Tamada, Y. Sulfation of silk fibroin by chlorosulfonic acid and the anticoagulant activity. Biomaterials 25, 377-383 (2004). (Pubitemid 37329688)
7. Murphy, A. R., John, P. S. & Kaplan, D. L. Modification of silk fibroin using diazonium coupling chemistry and the effects on hMCS proliferation and differentiation. Biomaterials 29, 2829-2838 (2008).
8. Sofia, S., McCarthy, M. B., Gronowicz, G. & Kaplan, D. L. Functionalized silkbased biomaterials for bone formation. J. Biomed. Mater. Res. 54, 139-148 (2001).
9. Meinel, L. et al. Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. J. Biomed. Mater. Res. A. 71, 25-34 (2004). (Pubitemid 39244392)
10. Karageorgiou, V. et al. Bone morphogenetic protein-2 decorated silk fibroin films induce osteogenic differentiation of human bone marrow stromal cells. J. Biomed. Mater. Res. A. 71, 528-537 (2004). (Pubitemid 39489907)
11. Ogawa, S., Tomita, M., Shimizu, K. & Yoshizato, K. Generation of a transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon: production of recombinant human serum albumin. J. Biotechnol. 128, 531-544 (2007). (Pubitemid 46135790)
12. Iizuka, M. et al. Production of a recombinant mouse monoclonal antibody in transgenic silkworm cocoons. FEBS J. 276, 5806-5820 (2009).
13. Tomita, M. et al Transgenic silkworms produce recombinant human type III procollagen in cocoons. Nat. Biotechnol. 21, 52-56 (2003).
14. Inoue, S. et al. A fibroin secretion-deficient silkworm mutant, Nd-sD, provides an efficient system for producing recombinant proteins. Insect Biochem. Mol. Biol. 35, 51-59 (2005). (Pubitemid 40015511)
15. Hino, R., Tomita, M. & Yoshizato, K. The generation of germline transgenic silkworms for the production of biologically active recombinant fusion proteins of fibroin and human basic fibroblast growth factor. Biomaterials 27, 5715-5724 (2006). (Pubitemid 44279069)
16. Kojima, K. et al. A new method for the modification of fibroin heavy chain protein in the transgenic silkworm. Biosci. Biotechnol. Biochem. 71, 2943-2951 (2007).
17. Bird, R. E. et al. Single-chain antigen-binding proteins. Science 242, 423-426 (1988).
18. Huston, J. S. et al. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc. Natl. Acad. Sci. USA 85, 5879-5883 (1988).
19. Sato, M. et al. Intrabodies against the EVH1 domain of Wiskott-Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells. FEBS J. 272, 6131-6144 (2005). (Pubitemid 41713702)
20. Derry, J. M., Ochs, H. D. & Francke, U. Isolation of a novel gene mutated in Wiskott-Aldrich syndrome. Cell 78, 635-644 (1994). (Pubitemid 24268586)
21. Thrasher, A. J. WASp in immune-system organization and function. Nat. Rev. Immunol. 2, 635-646 (2002). (Pubitemid 37328721)
22. Sato, M., Sawahata, R., Takenouchi, T. & Kitani, H. Identification of Fyn as the binding partner for the WASP N-terminal domain in T cells. Int. Immunol. 23, 493-502 (2011).
23. Sato, M. et al. Production of scFv-conjugated affinity silk powder by transgenic silkworm technology. PLoS ONE 7, e34632 (2012).
24. Gupta, V., Aseh, A., Rios, C. N., Aggarwal, B. B. & Mathur, A. B. Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy. Int. J. Nanomed. 4, 115-122 (2009).
25. Mathur, A. B. & Gupta, V. Silk fibroin-derived nanoparticles for biomedical applications. Nanomedicine 5, 807-820 (2010).
26. Cheema, S. K. et al. Silk fibroin mediated delivery of liposomal emodin to breast cancer cells. Int. J. Pharm. 341, 221-229 (2007). (Pubitemid 47088100)
27. Gobin, A. S., Rhea, R., Newman, R. A. & Mathur, A. B. Silk-fibroin-coated liposomes for long-term and targeted drug delivery. Int. J. Nanomed. 1, 81-87 (2006).
28. Kundu, J., Chung, Y., Kim, Y. H., Tae, G. & Kundu, S. C. Silk fibroin nanoparticles for cellular uptake and control release. Int. J. Pharm. 388, 242-250 (2010).
29. Ralph, P. & Nakoinz, I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119, 950-954 (1977). (Pubitemid 8191500)
30. Raschke, W. C., Baird, S., Ralph, P. & Nakoinz, I. Functional macrophage cell lines transformed by abelson leukemia virus. Cell 15, 261-267 (1978). (Pubitemid 9027529)