Combination of microwell structures and direct oxygenation enables efficient and size-regulated aggregate formation of an insulin-secreting pancreatic β-cell line

Biotechnology Progress

Volumn 30, Issue 1, 2014, Pages 178-187

  Shinohara, Marie ^a   Kimura, Hiroshi ^a   Montagne, Kevin ^a   Komori, Kikuo ^a   Fujii, Teruo ^a   Sakai, Yasuyuki ^a  

^a INSTITUTE OF INDUSTRIAL SCIENCE   (Japan)

Author keywords

Honeycomb microwell sheet; Insulin secretion; Oxygenation; Pancreatic cell; Spherical aggregates

Indexed keywords

CELLULAR ORGANIZATION; CONFLUENT MONOLAYERS; INSULIN SECRETION; MICRO WELLS; MOUSE INSULINOMA CELLS; POLYDIMETHYLSILOXANE PDMS; REGENERATIVE MEDICINE; SPHERICAL AGGREGATES;

CELL CULTURE; CELLS; HONEYCOMB STRUCTURES; INSULIN; MICROCHANNELS; OXYGEN SUPPLY; OXYGENATION; PHYSIOLOGY; SILICONES; THREE DIMENSIONAL;

AGGREGATES;

BAYSILON; DIMETICONE; INSULIN; LACTIC ACID; OXYGEN;

ANIMAL; ARTICLE; CELL SURVIVAL; CULTURE TECHNIQUE; CYTOLOGY; EQUIPMENT; EQUIPMENT DESIGN; HONEYCOMB MICROWELL SHEET; INSULIN RELEASE; METABOLISM; METHODOLOGY; MOUSE; MULTICELLULAR SPHEROID; OXYGENATION; PANCREAS ISLET BETA CELL; PANCREATIC Β-CELL; SPHERICAL AGGREGATES; TUMOR CELL LINE;

HONEYCOMB MICROWELL SHEET; INSULIN SECRETION; OXYGENATION; PANCREATIC Β-CELL; SPHERICAL AGGREGATES;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL SURVIVAL; DIMETHYLPOLYSILOXANES; EQUIPMENT DESIGN; INSULIN; INSULIN-SECRETING CELLS; LACTIC ACID; MICE; OXYGEN; SPHEROIDS, CELLULAR;

EID: 84893817078     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1837     Document Type: Article

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