PA tag: A versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin

Protein Expression and Purification

Volumn 95, Issue , 2014, Pages 240-247

  Fujii, Yuki ^a   Kaneko, Mika ^b   Neyazaki, Makiko ^c   Nogi, Terukazu ^c   Kato, Yukinari ^b   Takagi, Junichi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b TOHOKU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Immunoaffinity chromatography; Immunodetection; Mammalian cell expression; Monoclonal antibody; Peptide tag; Podoplanin; Purification method

Indexed keywords

HYBRID PROTEIN; MEMBRANE PROTEIN; MONOCLONAL ANTIBODY; OLIGOPEPTIDE; PDPN PROTEIN, HUMAN; PODOPLANIN;

ARTICLE; CHEMISTRY; EQUIPMENT; HUMAN; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNODETECTION; INTERFEROMETRY; ISOLATION AND PURIFICATION; KINETICS; MAMMALIAN CELL EXPRESSION; METABOLISM; METHODOLOGY; PEPTIDE TAG; PROTEIN BINDING; PURIFICATION METHOD;

IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNODETECTION; MAMMALIAN CELL EXPRESSION; MONOCLONAL ANTIBODY; PEPTIDE TAG; PODOPLANIN; PURIFICATION METHOD;

ANTIBODIES, MONOCLONAL; HUMANS; IMMUNOCHROMATOGRAPHY; INTERFEROMETRY; KINETICS; MEMBRANE GLYCOPROTEINS; OLIGOPEPTIDES; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS;

EID: 84893717643     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2014.01.009     Document Type: Article

References (22)

1. E. Hochuli, W. Bannwarth, H. Döbeli, R. Gentz, and D. Stuber Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent Nat. Biotechnol. 6 1988 1321 1325 (Pubitemid 18266749)
2. T.P. Hopp, K.S. Prickett, V.L. Price, R.T. Libby, C.J. March, D.P. Cerretti, D.L. Urdal, and P.J. Conlon A short polypeptide marker sequence useful for recombinant protein identification and purification Nat. Biotechnol. 6 1988 1204 1210
3. J. Field, J. Nikawa, D. Broek, B. MacDonald, L. Rodgers, I.A. Wilson, R.A. Lerner, and M. Wigler Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method Mol. Cell. Biol. 8 1988 2159 2165
4. G.I. Evan, G.K. Lewis, G. Ramsay, and J.M. Bishop Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product Mol. Cell. Biol. 5 1985 3610 3616 (Pubitemid 16233068)
5. S. Tabata, M. Nampo, E. Mihara, K. Tamura-Kawakami, I. Fujii, and J. Takagi A rapid screening method for cell lines producing singly-tagged recombinant proteins using the "TARGET tag" system J. Proteomics 73 2010 1777 1785
6. R.R. Burgess, and N.E. Thompson Advances in gentle immunoaffinity chromatography Curr. Opin. Biotechnol. 13 2002 304 308 (Pubitemid 35254086)
7. J.J. Lichty, J.L. Malecki, H.D. Agnew, D.J. Michelson-Horowitz, and S. Tan Comparison of affinity tags for protein purification Protein Expr. Purif. 41 2005 98 105 (Pubitemid 40406368)
8. A. Einhauer, and A. Jungbauer The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins J. Biochem. Biophys. Methods 49 2001 455 465 (Pubitemid 33022564)
9. Y. Kato, N. Fujita, A. Kunita, S. Sato, M. Kaneko, M. Osawa, and T. Tsuruo Molecular identification of Aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors J. Biol. Chem. 278 2003 51599 51605 (Pubitemid 38020404)
10. F. Sasaki, T. Okuno, K. Saeki, L. Min, N. Onohara, H. Kato, T. Shimizu, and T. Yokomizo A high-affinity monoclonal antibody against the FLAG tag useful for G-protein-coupled receptor study Anal. Biochem. 425 2012 157 165
11. T. Nogi, T. Sangawa, S. Tabata, M. Nagae, K. Tamura-Kawakami, A. Beppu, M. Hattori, N. Yasui, and J. Takagi Novel affinity tag system using structurally defined antibody-tag interaction: application to single-step protein purification Protein Sci. 17 2008 2120 2126
12. Y. Kato, M.K. Kaneko, A. Kuno, N. Uchiyama, K. Amano, Y. Chiba, Y. Hasegawa, J. Hirabayashi, H. Narimatsu, K. Mishima, and M. Osawa Inhibition of tumor cell-induced platelet aggregation using a novel anti-podoplanin antibody reacting with its platelet-aggregation-stimulating domain Biochem. Biophys. Res. Commun. 349 2006 1301 1307 (Pubitemid 44416399)
13. Y. Kato, M.K. Kaneko, A. Kunita, H. Ito, A. Kameyama, S. Ogasawara, N. Matsuura, Y. Hasegawa, K. Suzuki-Inoue, O. Inoue, Y. Ozaki, and H. Narimatsu Molecular analysis of the pathophysiological binding of the platelet aggregation-inducing factor podoplanin to the C-type lectin-like receptor CLEC-2 Cancer Sci. 99 2008 54 61 (Pubitemid 350239031)
14. M.K. Kaneko, A. Kunita, S. Abe, Y. Tsujimoto, M. Fukayama, K. Goto, Y. Sawa, Y. Nishioka, and Y. Kato Chimeric anti-podoplanin antibody suppresses tumor metastasis through neutralization and antibody-dependent cellular cytotoxicity Cancer Sci. 103 2012 1913 1919
15. M. Kato Kaneko, S. Ogasawara, and Y. Kato Establishment of a multi-specific monoclonal antibody MsMab-1 recognizing both IDH1 and IDH2 mutations Tohoku J. Exp. Med. 230 2013 103 109
16. P.J. Reeves, N. Callewaert, R. Contreras, and H.G. Khorana Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N- acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line Proc. Natl. Acad. Sci. USA 99 2002 13419 13424 (Pubitemid 35215396)
17. B.L. Brizzard, R.G. Chubet, and D.L. Vizard Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution Biotechniques 16 1994 730 735 (Pubitemid 2067227)
18. S. Chan, H. Gabra, F. Hill, G. Evan, and K. Sikora A novel tumour marker related to the c-myc oncogene product Mol. Cell. Probes 1 1987 73 82
19. S. Ogasawara, M.K. Kaneko, J.E. Price, and Y. Kato Characterization of anti-podoplanin monoclonal antibodies: critical epitopes for neutralizing the interaction between podoplanin and CLEC-2 Hybridoma (Larchmt) 27 2008 259 267
20. X. Liu, Y. Kato, M. Kato-Kaneko, M. Sugawara, S. Ogasawara, Y. Tsujimoto, Y. Naganuma, M. Yamakawa, T. Tsuchiya, and M. Takagi Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi-specific monoclonal antibody MsMab-1 Cancer Med. 2 2013 803 814
21. A. Marks, D.R. Sutherland, D. Bailey, J. Iglesias, J. Law, M. Lei, H. Yeger, D. Banerjee, and R. Baumal Characterization and distribution of an oncofetal antigen (M2A antigen) expressed on testicular germ cell tumours Br. J. Cancer 80 1999 569 578 (Pubitemid 29213215)
22. M.K. Kaneko, Y. Kato, A. Kameyama, H. Ito, A. Kuno, J. Hirabayashi, T. Kubota, K. Amano, Y. Chiba, Y. Hasegawa, I. Sasagawa, K. Mishima, and H. Narimatsu Functional glycosylation of human podoplanin: glycan structure of platelet aggregation-inducing factor FEBS Lett. 581 2007 331 336 (Pubitemid 46096478)