메뉴 건너뛰기




Volumn , Issue 84, 2014, Pages

Rapid and efficient zebrafish genotyping using PCR with high-resolution melt analysis

Author keywords

Basic Protocol; Genotyping; High resolution melting analysis (HRMA); Issue 84; Mutation; PCR; Transgenes; Zebrafish

Indexed keywords

DNA;

EID: 84893604172     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51138     Document Type: Article
Times cited : (29)

References (18)
  • 1
    • 4344636300 scopus 로고    scopus 로고
    • A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish
    • Kawakami, K., Takeda, H., Kawakami, N., Kobayashi, M., Matsuda, N. & Mishina, M. A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Dev. Cell. 7(1), 133-144 (2004).
    • (2004) Dev. Cell. , vol.7 , Issue.1 , pp. 133-144
    • Kawakami, K.1    Takeda, H.2    Kawakami, N.3    Kobayashi, M.4    Matsuda, N.5    Mishina, M.6
  • 2
    • 36448963734 scopus 로고    scopus 로고
    • The Tol2kit: A multisite gateway-based construction kit for Tol2 transposon transgenesis constructs
    • Kwan, K. M., Fujimoto, E., et al. The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev. Dyn. 236(11), 3088-3099 (2007).
    • (2007) Dev. Dyn. , vol.236 , Issue.11 , pp. 3088-3099
    • Kwan, K.M.1    Fujimoto, E.2
  • 3
    • 79961192836 scopus 로고    scopus 로고
    • Targeted gene disruption in somatic zebrafish cells using engineered TALENs
    • Sander, J. D., Cade, L., et al. Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat. Biotechnol. 29(8), 697-698 (2011).
    • (2011) Nat. Biotechnol. , vol.29 , Issue.8 , pp. 697-698
    • Sander, J.D.1    Cade, L.2
  • 4
    • 79961185942 scopus 로고    scopus 로고
    • Heritable gene targeting in zebrafish using customized TALENs
    • Huang, P., Xiao, A., Zhou, M., Zhu, Z., Lin, S. & Zhang, B. Heritable gene targeting in zebrafish using customized TALENs. Nat. Biotechnol. 29(8), 699-700 (2011).
    • (2011) Nat. Biotechnol. , vol.29 , Issue.8 , pp. 699-700
    • Huang, P.1    Xiao, A.2    Zhou, M.3    Zhu, Z.4    Lin, S.5    Zhang, B.6
  • 5
    • 66349090454 scopus 로고    scopus 로고
    • High-resolution melting analysis (HRMA): More than just sequence variant screening
    • Vossen, R. H. A. M., Aten, E., Roos, A. & Den Dunnen, J. T. High-resolution melting analysis (HRMA): more than just sequence variant screening. Hum. Mutation. 30(6), 860-866 (2009).
    • (2009) Hum. Mutation. , vol.30 , Issue.6 , pp. 860-866
    • Vossen, R.H.A.M.1    Aten, E.2    Roos, A.3    Den Dunnen, J.T.4
  • 6
    • 84866156176 scopus 로고    scopus 로고
    • Simple methods for generating and detecting locus-specific mutations induced with TALENs in the zebrafish genome
    • Dahlem, T. J., Hoshijima, K., et al. Simple methods for generating and detecting locus-specific mutations induced with TALENs in the zebrafish genome. PLoS Genet. 8(8), e1002861 (2012).
    • (2012) PLoS Genet. , vol.8 , Issue.8
    • Dahlem, T.J.1    Hoshijima, K.2
  • 7
    • 3042651880 scopus 로고    scopus 로고
    • Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons
    • Liew, M., Pryor, R., et al. Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. Clin. Chem. 50(7), 1156-1164 (2004).
    • (2004) Clin. Chem. , vol.50 , Issue.7 , pp. 1156-1164
    • Liew, M.1    Pryor, R.2
  • 8
    • 33644548649 scopus 로고    scopus 로고
    • V Amplicon DNA melting analysis for mutation scanning and genotyping: Cross-platform comparison of instruments and dyes
    • Herrmann, M. G., Durtschi, J. D., Bromley, L. K., Wittwer, C. T. & Voelkerding, K. V Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes. Clin. Chem. 52(3), 494-503 (2006).
    • (2006) Clin. Chem. , vol.52 , Issue.3 , pp. 494-503
    • Herrmann, M.G.1    Durtschi, J.D.2    Bromley, L.K.3    Wittwer, C.T.4    Voelkerding, K.5
  • 9
    • 84865969331 scopus 로고    scopus 로고
    • High resolution melting analysis for the identification of novel mutations in DKC1 and TERT genes in patients with dyskeratosis congenita
    • Carrillo, J., Martínez, P., et al. High resolution melting analysis for the identification of novel mutations in DKC1 and TERT genes in patients with dyskeratosis congenita. Blood Cell. Mol. Dis. 49(3-4), 140-146 (2012).
    • (2012) Blood Cell. Mol. Dis. , vol.49 , Issue.3-4 , pp. 140-146
    • Carrillo, J.1    Martínez, P.2
  • 10
    • 84866557902 scopus 로고    scopus 로고
    • High resolution melt analysis (HRMA); a viable alternative to agarose gel electrophoresis for mouse genotyping
    • Thomsen, N., Ali, R. G., Ahmed, J. N. & Arkell, R. M. High resolution melt analysis (HRMA); a viable alternative to agarose gel electrophoresis for mouse genotyping. PloS one. 7(9), e45252 (2012).
    • (2012) PloS one. , vol.7 , Issue.9
    • Thomsen, N.1    Ali, R.G.2    Ahmed, J.N.3    Arkell, R.M.4
  • 11
    • 77956839940 scopus 로고    scopus 로고
    • PIK3CA hotspot mutation scanning by a novel and highly sensitive high-resolution small amplicon melting analysis method
    • Vorkas, P. A., Poumpouridou, N., Agelaki, S., Kroupis, C., Georgoulias, V. & Lianidou, E. S. PIK3CA hotspot mutation scanning by a novel and highly sensitive high-resolution small amplicon melting analysis method. J. Mol. Diagn. 12(5), 697-704 (2010).
    • (2010) J. Mol. Diagn. , vol.12 , Issue.5 , pp. 697-704
    • Vorkas, P.A.1    Poumpouridou, N.2    Agelaki, S.3    Kroupis, C.4    Georgoulias, V.5    Lianidou, E.S.6
  • 12
    • 70949104150 scopus 로고    scopus 로고
    • A rapid and efficient method of genotyping zebrafish mutants
    • Parant, J. M., George, S. A., Pryor, R., Wittwer, C. T. & Yost, H. J. A rapid and efficient method of genotyping zebrafish mutants. Dev. Dyn. 238(12), 3168-3174 (2009).
    • (2009) Dev. Dyn. , vol.238 , Issue.12 , pp. 3168-3174
    • Parant, J.M.1    George, S.A.2    Pryor, R.3    Wittwer, C.T.4    Yost, H.J.5
  • 13
    • 84866595979 scopus 로고    scopus 로고
    • Zebrafish foxP2 zinc finger nuclease mutant has normal axon pathfinding
    • Xing, L., Hoshijima, K., et al. Zebrafish foxP2 zinc finger nuclease mutant has normal axon pathfinding. PloS one. 7(8), e43968 (2012).
    • (2012) PloS one , vol.7 , Issue.8
    • Xing, L.1    Hoshijima, K.2
  • 14
    • 80055000226 scopus 로고    scopus 로고
    • Evaluation of High Resolution Melting analysis as an alternate tool to screen for risk alleles associated with small kidneys in Indian newborns
    • Raghavendra, A., Siji, A., Sridhar, T. S., Phadke, K. & Vasudevan, A. Evaluation of High Resolution Melting analysis as an alternate tool to screen for risk alleles associated with small kidneys in Indian newborns. BMC Nephrol. 12(1), 60 (2011).
    • (2011) BMC Nephrol. , vol.12 , Issue.1 , pp. 60
    • Raghavendra, A.1    Siji, A.2    Sridhar, T.S.3    Phadke, K.4    Vasudevan, A.5
  • 15
    • 34250633774 scopus 로고    scopus 로고
    • V Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping
    • Herrmann, M. G., Durtschi, J. D., Wittwer, C. T. & Voelkerding, K. V Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping. Clin. Chem. 53(8), 1544-1548 (2007).
    • (2007) Clin. Chem. , vol.53 , Issue.8 , pp. 1544-1548
    • Herrmann, M.G.1    Durtschi, J.D.2    Wittwer, C.T.3    Voelkerding, K.4
  • 16
    • 0038359432 scopus 로고    scopus 로고
    • High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen
    • Wittwer, C. T. High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen. Clin. Chem.49(6), 853-860 (2003).
    • (2003) Clin. Chem. , vol.49 , Issue.6 , pp. 853-860
    • Wittwer, C.T.1
  • 17
    • 84867660686 scopus 로고    scopus 로고
    • A closed-tube methylation-sensitive high resolution melting assay (MS-HRMA) for the semi-quantitative determination of CST6 promoter methylation in clinical samples
    • Dimitrakopoulos, L., Vorkas, P. A., Georgoulias, V. & Lianidou, E. S. A closed-tube methylation-sensitive high resolution melting assay (MS-HRMA) for the semi-quantitative determination of CST6 promoter methylation in clinical samples. BMC Cancer. 12, 486 (2012).
    • (2012) BMC Cancer. , vol.12 , pp. 486
    • Dimitrakopoulos, L.1    Vorkas, P.A.2    Georgoulias, V.3    Lianidou, E.S.4
  • 18
    • 84866450325 scopus 로고    scopus 로고
    • Comparative analysis of two broad-range PCR assays for pathogen detection in positive-blood-culture bottles: PCR-high-resolution melting analysis versus PCR-mass spectrometry
    • Jeng, K., Gaydos, C. A., et al. Comparative analysis of two broad-range PCR assays for pathogen detection in positive-blood-culture bottles: PCR-high-resolution melting analysis versus PCR-mass spectrometry. J. Clin. Microbiol. 50(10), 3287-3292 (2012).
    • (2012) J. Clin. Microbiol. , vol.50 , Issue.10 , pp. 3287-3292
    • Jeng, K.1    Gaydos, C.A.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.