Comparative analysis of two broad-range PCR assays for pathogen detection in positive-blood-culture bottles: PCR-high-resolution melting analysis versus PCR-mass spectrometry

Journal of Clinical Microbiology

Volumn 50, Issue 10, 2012, Pages 3287-3292

  Jeng, Kevin ^a,b   Gaydos, Charlotte A ^a   Blyn, Lawrence B ^c   Yang, Samuel ^a   Won, Helen ^a   Matthews, Heather ^c   Toleno, Donna ^c   Hsieh, Yu Hsiang ^a   Carroll, Karen C ^a   Hardick, Justin ^a   Masek, Billy ^a   Kecojevic, Alexander ^a   Sampath, Rangarajan ^c   Peterson, Stephen ^a   Rothman, Richard E ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b DUKE UNIVERSITY   (United States)

^c ABBOTT LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA HEMOLYTIC STREPTOCOCCUS; ARTICLE; BLOOD CULTURE; CITROBACTER FREUNDII; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; ELECTROSPRAY MASS SPECTROMETRY; ENTEROBACTER AEROGENES; ENTEROBACTER CLOACAE; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ESCHERICHIA COLI; FUSOBACTERIUM VARIUM; GOLD STANDARD; HAEMOPHILUS INFLUENZAE; HIGH RESOLUTION MELTING ANALYSIS; HUMAN; INTERMETHOD COMPARISON; KLEBSIELLA PNEUMONIAE; MICROBIAL IDENTIFICATION; MICROBIOLOGICAL EXAMINATION; MICROCOCCUS LUTEUS; MICROORGANISM DETECTION; MORGANELLA MORGANII; NONHUMAN; PILOT STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; RETROSPECTIVE STUDY; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS PYOGENES;

BLOOD; HUMANS; MASS SPECTROMETRY; MICROBIOLOGICAL TECHNIQUES; MOLECULAR DIAGNOSTIC TECHNIQUES; NUCLEIC ACID DENATURATION; PILOT PROJECTS; POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; SEPSIS; TRANSITION TEMPERATURE;

EID: 84866450325     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00677-12     Document Type: Article

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