Dental caries and vaccination strategy against the major cariogenic pathogen, Streptococcus mutans

Current Pharmaceutical Biotechnology

Volumn 14, Issue 11, 2013, Pages 960-966

  Zhang, Song ^a  

^a Department of Dentistry Taishan Sanatorium of Shandong Province ^*  (China)

Author keywords

Biofilm; Dental caries; Streptococcus mutans; Subunit vaccines

Indexed keywords

DNA VACCINE; EXOPOLYSACCHARIDE; GLUCOSYLTRANSFERASE; MONOCLONAL ANTIBODY; RECOMBINANT VACCINE; TOLL LIKE RECEPTOR 2; TOLL LIKE RECEPTOR 4;

ACTINOMYCES VISCOSUS; BIOFILM; CROSS REACTION; DENTAL CARIES; DNA IMMUNIZATION; ENAMEL; GINGIVITIS; HUMAN; LACTOBACILLUS ACIDOPHILUS; MEDICAL RESEARCH; NONHUMAN; PASSIVE IMMUNIZATION; PERIIMPLANTITIS; PERIPHERAL BLOOD MONONUCLEAR CELL; QUORUM SENSING; REVIEW; RHEUMATIC FEVER; SALMONELLA TYPHIMURIUM; STREPTOCOCCUS GORDONII; STREPTOCOCCUS MUTANS; STREPTOCOCCUS SOBRINUS; VACCINATION;

BACTERIAL VACCINES; DENTAL CARIES; DRUG COMPOUNDING; DRUG DESIGN; HUMANS; STREPTOCOCCAL INFECTIONS; STREPTOCOCCUS MUTANS; TREATMENT OUTCOME; VACCINATION;

EID: 84893499947     PISSN: 13892010     EISSN: 18734316     Source Type: Journal    
DOI: 10.2174/1389201014666131226144339     Document Type: Review

References (95)

1. Shivakumar, K. M.; Vidya, S. K.; Chandu, G. N. Dental caries vaccine. Indian J. Dent. Res., 2009, 20(1), 99-106.
2. Hajishengallis, G.; Russell, M. W.; Michalek, S. M. Comparison of an adherence domain and a structural region of Streptococcus mutans antigen I/II in protective immunity against dental caries in rats after intranasal immunization. Infect. Immun., 1998, 66(4), 1740-1743.
3. Chen, F.; Wang, D. Novel technologies for the prevention and treatment of dental caries: A patent survey. Expert. Opin. Ther. Pat., 2010, 20(5), 681-694.
4. Selwitz, R. H.; Ismail, A. I.; Pitts, N. B., Dental caries. Lancet, 2007, 369(9555), 51-59.
5. Featherstone, J. D. The science and practice of caries prevention. J. Am. Dent. Assoc., 2000, 131(7), 887-899.
6. Featherstone, J. D., Prevention and reversal of dental caries: role of low level fluoride. Community Dent. Oral Epidemiol., 1999, 27(1), 31-40.
7. Garcia-Godoy, F.; Hicks, M. J. Maintaining the integrity of the enamel surface: the role of dental biofilm, saliva and preventive agents in enamel demineralization and remineralization. J. Am. Dent. Assoc., 2008, 139(Suppl), 25S-34S.
8. Yan, H. Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine. Sci. China Life Sci., 2013, 56(5), 406-413.
9. Raoult, D.; Foti, B.; Aboudharam, G. Historical and geographical parallelism between the incidence of dental caries, Streptococcus mutans and sugar intake. Eur. J. Epidemiol., 2013.
10. Petersen, P. E.; Bourgeois, D.; Ogawa, H.; Estupinan-Day, S.; Ndiaye, C. The global burden of oral diseases and risks to oral health. Bull World Health Organ, 2005, 83(9), 661-669.
11. Hirose, H.; Hirose, K.; Isogai, E.; Miura, H.; Ueda, I. Close association between Streptococcus sobrinus in the saliva of young children and smooth-surface caries increment. Caries Res., 1993, 27(4), 292-297.
12. Holbrook, W. P.; Magnusdottir, M. O. Studies on strains of Streptococcus mutans isolated from caries-active and caries-free individuals in Iceland. J. Oral Microbiol., 2012, 4, 10611.
13. Zickert, I.; Emilson, C. G.; Krasse, B. Streptococcus mutans, lactobacilli and dental health in 13-14-year-old Swedish children. Community Dent. Oral Epidemiol., 1982, 10(2), 77-81.
14. Berkowitz, R. J. Mutans streptococci: Acquisition and transmission. Pediatr Dent, 2006, 28(2), 106-109; discussion 192-108.
15. Alaluusua, S. Transmission of mutans streptococci. Proc. Finn. Dent. Soc., 1991, 87(4), 443-447.
16. Marinho, V. C.; Higgins, J. P.; Logan, S.; Sheiham, A. Topical fluoride (toothpastes, mouthrinses, gels or varnishes) for preventing dental caries in children and adolescents. Cochrane Database Syst. Rev., 2003, (4), CD002782.
17. Li, P.; Xue, Y.; Zhang, W.; Teng, F.; Sun, Y.; Qu, T.; Chen, X.; Cheng, X.; Song, B.; Luo, W.; Yu, Q. Sodium fluoride induces apoptosis in odontoblasts via a JNK-dependent mechanism. Toxicology, 2013, 308, 138-145.
18. Richter, H.; Kierdorf, U.; Richards, A.; Kierdorf, H. Dentin abnormalities in cheek teeth of wild red deer and roe deer from a fluoride-polluted area in Central Europe. Ann. Anat., 2010, 192(2), 86-95.
19. de Almeida Baldini Cardoso, C.; Mangueira, D. F.; Olympio, K. P.; Magalhaes, A. C.; Rios, D.; Honorio, H. M.; Vilhena, F. V.; Sampaio, F. C.; Buzalaf, M. A. The effect of pH and fluoride concentration of liquid dentifrices on caries progression. Clin. Oral Investig., 2013.
20. Isokangas, P.; Soderling, E.; Pienihakkinen, K.; Alanen, P. Occurrence of dental decay in children after maternal consumption of xylitol chewing gum, a follow-up from 0 to 5 years of age. J. Dent. Res., 2000, 79(11), 1885-1889.
21. Thorild, I.; Lindau, B.; Twetman, S. Caries in 4-year-old children after maternal chewing of gums containing combinations of xylitol, sorbitol, chlorhexidine and fluoride. Eur. Arch. Paediatr Dent., 2006, 7(4), 241-245.
22. Fontana, M.; Catt, D.; Eckert, G. J.; Ofner, S.; Toro, M.; Gregory, R. L.; Zandona, A. F.; Eggertsson, H.; Jackson, R.; Chin, J.; Zero, D.; Sissons, C. H. Xylitol: Effects on the acquisition of cariogenic species in infants. Pediatr. Dent., 2009, 31(3), 257-266.
23. Makinen, K. K.; Jarvinen, K. L.; Anttila, C. H.; Luntamo, L. M.; Vahlberg, T. Topical xylitol administration by parents for the promotion of oral health in infants: A caries prevention experiment at a Finnish Public Health Centre. Int. Dent. J., 2013, 63(4), 210-224.
24. Tran, P.; Hamood, A.; Mosley, T.; Gray, T.; Jarvis, C.; Webster, D.; Amaechi, B.; Enos, T.; Reid, T. Organo-selenium-containing dental sealant inhibits bacterial biofilm. J. Dent. Res., 2013, 92(5), 461-466.
25. Besinis, A.; De Peralta, T.; Handy, R. Inhibition of biofilm formation and antibacterial properties of a silver nano-coating on human dentine. Nanotoxicology, 2014, 745-754.
26. Li, H.; Cheng, J. W.; Yu, H. Y.; Xin, Y.; Tang, L.; Ma, Y. Effect of the antimicrobial peptide D-Nal-Pac-525 on the growth of Streptococcus mutans and its biofilm formation. J. Microbiol. Biotechnol., 2013, 23(8), 1070-1075.
27. Burton, J. P.; Drummond, B. K.; Chilcott, C. N.; Tagg, J. R.; Thomson, W. M.; Hale, J. D.; Wescombe, P. A. Influence of the probiotic Streptococcus salivarius strain M18 on indices of dental health in children: A randomized double-blind, placebo-controlled trial. J. Med. Microbiol., 2013, 62(Pt 6), 875-884.
28. Güngör, O. E.; Ki{dotless}rzi{dotless}oǎlu, Z.; Dinçer, E.; Ki{dotless}vanç, M. Who will win the race in childrens' oral cavities? Streptococcus mutans or beneficial lactic acid bacteria? Benef. Microbes, 2013, 4(3), 237-245.
29. Sudhir, R.; Praveen, P.; Anantharaj, A.; Venkataraghavan, K. Assessment of the effect of probiotic curd consumption on salivary pH and Streptococcus mutans counts. Niger. Med. J., 2012, 53(3), 135-139.
30. Juneja, A.; Kakade, A. Evaluating the effect of probiotic containing milk on salivary mutans streptococci levels. J. Clin. Pediatr Dent., 2012, 37(1), 9-14.
31. Soderlund, K. Take two minutes to brush. Ad Council launches campaign on children's oral health. By Kelly Soderlund, American Dental Association News staff. http://www. ada. org/news/7484. aspx. 2012.
32. O'Hehir, T. E. Hygiene & prevention profile in oral health-the new standard for prevention. Dentaltown. com, 2011, 102-104.
33. ten Cate, J. M. Biofilms, a new approach to the microbiology of dental plaque. Odontology, 2006, 94(1), 1-9.
34. Nyvad, B.; Kilian, M. Microbiology of the early colonization of human enamel and root surfaces in vivo. Scand. J. Dent. Res., 1987, 95(5), 369-380.
35. Keller, M. K.; Twetman, S. Acid production in dental plaque after exposure to probiotic bacteria. BMC Oral Health, 2012, 12, 44.
36. Kõll, P.; Mändar, R.; Marcotte, H.; Leibur, E.; Mikelsaar, M.; Hammarström, L. Characterization of oral lactobacilli as potential probiotics for oral health. Oral Microbiol. Immunol., 2008, 23(2), 139-147.
37. Filoche, S. K.; Anderson, S. A.; Sissons, C. H. Biofilm growth of Lactobacillus species is promoted by Actinomyces species and Streptococcus mutans. Oral Microbiol. Immunol., 2004, 19(5), 322-326.
38. Masadeh, M. M.; Gharaibeh, S. F.; Alzoubi, K. H.; Al-Azzam, S. I.; Obeidat, W. M. Antimicrobial activity of common mouthwash solutions on multidrug-resistance bacterial biofilms. J. Clin. Med. Res., 2013, 5(5), 389-394.
39. Sun, J.; Song, X. Assessment of antimicrobial susceptibility of Enterococcus faecalis isolated from chronic periodontitis in biofilm versus planktonic phase. J. Periodontol., 2011, 82(4), 626-631.
40. Hojo, K.; Nagaoka, S.; Ohshima, T.; Maeda, N. Bacterial interactions in dental biofilm development. J. Dent. Res., 2009, 88(11), 982-990.
41. Koo, H.; Xiao, J.; Klein, M. I.; Jeon, J. G. Exopolysaccharides produced by Streptococcus mutans glucosyltransferases modulate the establishment of microcolonies within multispecies biofilms. J. Bacteriol., 2010, 192(12), 3024-3032.
42. Pratt, L. A.; Kolter, R. Genetic analyses of bacterial biofilm formation. Curr. Opin. Microbiol., 1999, 2(6), 598-603.
43. Costerton, J. W.; Lewandowski, Z.; Caldwell, D. E.; Korber, D. R.; Lappin-Scott, H. M. Microbial biofilms. Annu. Rev. Microbiol., 1995, 49, 711-745.
44. Dunny, G. M.; Leonard, B. A., Cell-cell communication in grampositive bacteria. Annu. Rev. Microbiol., 1997, 51, 527-564.
45. Syvitski, R. T.; Tian, X. L.; Sampara, K.; Salman, A.; Lee, S. F.; Jakeman, D. L.; Li, Y. H., Structure-activity analysis of quorumsensing signaling peptides from Streptococcus mutans. J. Bacteriol., 2007, 189(4), 1441-1450.
46. Li, Y. H.; Lau, P. C.; Lee, J. H.; Ellen, R. P.; Cvitkovitch, D. G., Natural genetic transformation of Streptococcus mutans growing in biofilms. J. Bacteriol., 2001, 183(3), 897-908.
47. Petersen, F. C.; Scheie, A. A. Genetic transformation in Streptococcus mutans requires a peptide secretion-like apparatus. Oral Microbiol. Immunol., 2000, 15(5), 329-334.
48. Perry, J. A.; Jones, M. B.; Peterson, S. N.; Cvitkovitch, D. G.; Levesque, C. M. Peptide alarmone signalling triggers an auto-active bacteriocin necessary for genetic competence. Mol. Microbiol., 2009, 72(4), 905-917.
49. Aspiras, M. B.; Ellen, R. P.; Cvitkovitch, D. G. ComX activity of Streptococcus mutans growing in biofilms. FEMS Microbiol Lett., 2004, 238(1), 167-174.
50. Cvitkovitch, D. G.; Li, Y. H.; Ellen, R. P. Quorum sensing and biofilm formation in streptococcal infections. J Clin Invest, 2003, 112(11), 1626-1632.
51. Li, Y. H.; Tang, N.; Aspiras, M. B.; Lau, P. C.; Lee, J. H.; Ellen, R. P.; Cvitkovitch, D. G. A quorum-sensing signaling system essential for genetic competence in Streptococcus mutans is involved in biofilm formation. J. Bacteriol., 2002, 184(10), 2699-2708.
52. Surette, M. G.; Miller, M. B.; Bassler, B. L. Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: A new family of genes responsible for autoinducer production. Proc. Natl. Acad. Sci. USA, 1999, 96(4), 1639-1644.
53. Schauder, S.; Bassler, B. L. The languages of bacteria. Genes Dev., 2001, 15(12), 1468-1480.
54. Merritt, J.; Qi, F.; Goodman, S. D.; Anderson, M. H.; Shi, W. Mutation of luxS affects biofilm formation in Streptococcus mutans. Infect. Immun., 2003, 71(4), 1972-1979.
55. Wen, Z. T.; Nguyen, A. H.; Bitoun, J. P.; Abranches, J.; Baker, H. V.; Burne, R. A. Transcriptome analysis of LuxS-deficient Streptococcus mutans grown in biofilms. Mol. Oral Microbiol., 2011, 26(1), 2-18.
56. Gong, Y.; Tian, X. L.; Sutherland, T.; Sisson, G.; Mai, J.; Ling, J.; Li, Y. H. Global transcriptional analysis of acid-inducible genes in Streptococcus mutans: multiple two-component systems involved in acid adaptation. Microbiology, 2009, 155(Pt 10), 3322-3332.
57. Marsh, P. D. Are dental diseases examples of ecological catastrophes? Microbiology, 2003, 149(Pt 2), 279-294.
58. Paes Leme, A. F.; Koo, H.; Bellato, C. M.; Bedi, G.; Cury, J. A. The role of sucrose in cariogenic dental biofilm formation-new insight. J. Dent. Res., 2006, 85, (10), 878-887.
59. Quivey, R. G.; Kuhnert, W. L.; Hahn, K. Genetics of acid adaptation in oral streptococci. Crit Rev Oral Biol Med, 2001, 12(4), 301-314.
60. Russell, M. W.; Lehner, T. Characterisation of antigens extracted from cells and culture fluids of Streptococcus mutans serotype C. Arch. Oral Biol., 1978, 23(1), 7-15.
61. Russell, R. R. Wall-associated protein antigens of Streptococcus mutans. J. Gen. Microbiol., 1979, 114(1), 109-115.
62. Forester, H.; Hunter, N.; Knox, K. W. Characteristics of a high molecular weight extracellular protein of Streptococcus mutans. J. Gen. Microbiol., 1983, 129(9), 2779-2788.
63. Koga, T.; Okahashi, N.; Takahashi, I.; Kanamoto, T.; Asakawa, H.; Iwaki, M. Surface hydrophobicity, adherence, and aggregation of cell surface protein antigen mutants of Streptococcus mutans serotype A. Infect. Immun., 1990, 58(2), 289-296.
64. Lis, M.; Shiroza, T.; Kuramitsu, H. K. Role of C-terminal direct repeating units of the Streptococcus mutans glucosyltransferase-S in glucan binding. Appl. Environ. Microbiol., 1995, 61(5), 2040-2042.
65. Li, Y. H.; Huang, S.; Du, M.; Bian, Z.; Chen, Z.; Fan, M. W. Immunogenic characterization and protection against Streptococcus mutans infection induced by intranasal DNA prime-protein boost immunization. Vaccine, 2010, 28(32), 5370-5376.
66. Sun, Y.; Shi, W.; Yang, J. Y.; Zhou, D. H.; Chen, Y. Q.; Zhang, Y.; Yang, Y.; He, B. X.; Zhong, M. H.; Li, Y. M.; Cao, Y.; Xiao, Y.; Li, W.; Yu, J.; Li, Y. H.; Fan, M. W.; Yan, H. M. Flagellin-PAc fusion protein is a high-efficacy anti-caries mucosal vaccine. J. Dent. Res., 2012, 91(10), 941-947.
67. Jia, R.; Guo, J. H.; Fan, M. W.; Bian, Z.; Chen, Z.; Fan, B.; Yu, F.; Xu, Q. A. Immunogenicity of CTLA4 fusion anti-caries DNA vaccine in rabbits and monkeys. Vaccine, 2006, 24(24), 5192-5200.
68. Chia, J. S.; You, C. M.; Hu, C. Y.; Chiang, B. L.; Chen, J. Y. Human T-cell responses to the glucosyltransferases of Streptococcus mutans. Clin. Diagn. Lab. Immunol., 2001, 8(2), 441-445.
69. Sun, J.; Yang, X.; Xu, Q. A.; Bian, Z.; Chen, Z.; Fan, M. Protective efficacy of two new anti-caries DNA vaccines. Vaccine, 2009, 27(52), 7459-7466.
70. Guo, J. H.; Jia, R.; Fan, M. W.; Bian, Z.; Chen, Z.; Peng, B. Construction and immunogenic characterization of a fusion anticaries DNA vaccine against PAc and glucosyltransferase I of Streptococcus mutans. J. Dent. Res., 2004, 83(3), 266-270.
71. Smith, D. J.; King, W. F.; Rivero, J.; Taubman, M. A. Immunological and protective effects of diepitopic subunit dental caries vaccines. Infect. Immun., 2005, 73(5), 2797-2804.
72. Xu, Q.; Katz, J.; Zhang, P.; Ashtekar, A. R.; Gaddis, D. E.; Fan, M.; Michalek, S. M. Contribution of a Streptococcus mutans antigen expressed by a Salmonella vector vaccine in dendritic cell activation. Infect. Immun., 2011, 79(9), 3792-3800.
73. Zhang, S.; Walters, N.; Cao, L.; Robison, A.; Yang, X. Recombinant Salmonella vaccination technology and its application to human bacterial pathogens. Curr. Pharm. Biotechnol., 2013, 14(2), 209-219.
74. Ma, J. K.; Smith, R.; Lehner, T. Use of monoclonal antibodies in local passive immunization to prevent colonization of human teeth by Streptococcus mutans. Infect. Immun., 1987, 55(5), 1274-1278.
75. Ma, J. K.; Hunjan, M.; Smith, R.; Lehner, T. Specificity of monoclonal antibodies in local passive immunization against Streptococcus mutans. Clin. Exp. Immunol., 1989, 77(3), 331-337.
76. Haigwood, N. L.; Watson, A.; Sutton, W. F.; McClure, J.; Lewis, A.; Ranchalis, J.; Travis, B.; Voss, G.; Letvin, N. L.; Hu, S. L.; Hirsch, V. M.; Johnson, P. R. Passive immune globulin therapy in the SIV/macaque model: Early intervention can alter disease profile. Immunol. Lett., 1996, 51(1-2), 107-114.
77. Brady, L. J., Antibody-mediated immunomodulation: a strategy to improve host responses against microbial antigens. Infect. Immun., 2005, 73(2), 671-678.
78. Robinette, R. A.; Oli, M. W.; McArthur, W. P.; Brady, L. J. A therapeutic anti-Streptococcus mutans monoclonal antibody used in human passive protection trials influences the adaptive immune response. Vaccine, 2011, 29(37), 6292-6300.
79. Chia, J. S.; Lin, R. H.; Lin, S. W.; Chen, J. Y.; Yang, C. S. Inhibition of glucosyltransferase activities of Streptococcus mutans by a monoclonal antibody to a subsequence peptide. Infect. Immun., 1993, 61(11), 4689-4695.
80. Kim, M. A.; Lee, M. J.; Jeong, H. K.; Song, H. J.; Jeon, H. J.; Lee, K. Y.; Kim, J. G. A monoclonal antibody specific to glucosyltransferase B of Streptococcus mutans GS-5 and its glucosyltransferase inhibitory efficiency. Hybridoma (Larchmt), 2012, 31(6), 430-435.
81. Kunze, B.; Reck, M.; Dotsch, A.; Lemme, A.; Schummer, D.; Irschik, H.; Steinmetz, H.; Wagner-Dobler, I. Damage of Streptococcus mutans biofilms by carolacton, a secondary metabolite from the myxobacterium Sorangium cellulosum. BMC Microbiol., 2010, 10, 199.
82. Ferretti, J. J.; Shea, C.; Humphrey, M. W. Cross-reactivity of Streptococcus mutans antigens and human heart tissue. Infect. Immun., 1980, 30(1), 69-73.
83. Yang, X.; Thornburg, T.; Holderness, K.; Suo, Z.; Cao, L.; Lim, T.; Avci, R.; Pascual, D. W. Serum antibodies protect against intraperitoneal challenge with enterotoxigenic Escherichia coli. J. Biomed. Biotechnol., 2011, 2011, 632396.
84. Millet, P.; Kalish, M. L.; Collins, W. E.; Hunter, R. L. Effect of adjuvant formulations on the selection of B-cell epitopes expressed by a malaria peptide vaccine. Vaccine, 1992, 10(8), 547-550.
85. Yang, X.; Hinnebusch, B. J.; Trunkle, T.; Bosio, C. M.; Suo, Z.; Tighe, M.; Harmsen, A.; Becker, T.; Crist, K.; Walters, N.; Avci, R.; Pascual, D. W. Oral vaccination with Salmonella simultaneously expressing Yersinia pestis F1 and V antigens protects against bubonic and pneumonic plague. J. Immunol., 2007, 178(2), 1059-1067.
86. Huang, L.; Xu, Q. A.; Liu, C.; Fan, M. W.; Li, Y. H. Anti-caries DNA vaccine-induced secretory immunoglobulin A antibodies inhibit formation of Streptococcus mutans biofilms in vitro. Acta Pharmacol. Sin., 2013, 34(2), 239-246.
87. Yang, X.; Suo, Z.; Thornburg, T.; Holderness, K.; Cao, L.; Lim, T.; Walters, N.; Kellerman, L.; Loetterle, L.; Avci, L.; Pascual, D. W. Expression of Escherichia coli virulence usher protein attenuates wild-type Salmonella. Virulence, 2012, 3(1), 29-41.
88. Yang, X.; Thornburg, T.; Suo, Z.; Jun, S.; Robison, A.; Li, J.; Lim, T.; Cao, L.; Hoyt, T.; Avci, R.; Pascual, D. W. Flagella overexpression attenuates Salmonella pathogenesis. PLoS One, 2012, 7, e46828.
89. Pascual, D. W.; Suo, Z.; Cao, L.; Avci, R.; Yang, X. Attenuating gene expression (AGE) for vaccine development. Virulence, 2013, 4(5), 384-390.
90. Cao, L.; Lim, T.; Jun, S.; Thornburg, T.; Avci, R.; Yang, X. Vulnerabilities in Yersinia pestis caf operon are unveiled by a Salmonella vector. PLoS One, 2012, 7(4), e36283.
91. Lemiale, F.; Kong, W. P.; Akyurek, L. M.; Ling, X.; Huang, Y.; Chakrabarti, B. K.; Eckhaus, M.; Nabel, G. J. Enhanced mucosal immunoglobulin A response of intranasal adenoviral vector human immunodeficiency virus vaccine and localization in the central nervous system. J. Virol., 2003, 77(18), 10078-10087.
92. Armstrong, M. E.; Lavelle, E. C.; Loscher, C. E.; Lynch, M. A.; Mills, K. H. Proinflammatory responses in the murine brain after intranasal delivery of cholera toxin: implications for the use of AB toxins as adjuvants in intranasal vaccines. J. Infect. Dis., 2005, 192(9), 1628-1633.
93. Jun, S.; Clapp, B.; Zlotkowska, D.; Hoyt, T.; Holderness, K.; Maddaloni, M.; Pascual, D. W. Sublingual immunization with adenovirus F protein-based vaccines stimulates protective immunity against botulinum neurotoxin A intoxication. Int. Immunol., 2012, 24(2), 117-128.
94. Sheiham, A. Dietary effects on dental diseases. Public Health Nutr., 2001, 4, (2B), 569-591.
95. Moynihan, P.; Petersen, P. E. Diet, nutrition and the prevention of dental diseases. Public Health Nutr., 2004, 7(1A), 201-226.