Critical residues of class II PHA synthase for expanding the substrate specificity and enhancing the biosynthesis of polyhydroxyalkanoate

Enzyme and Microbial Technology

Volumn 56, Issue , 2014, Pages 60-66

  Chen, Yi Jr ^a,b   Tsai, Pei Chien ^a   Hsu, Chun Hua ^a   Lee, Chia Yin ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b CHANG GUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Taiwan)

Author keywords

Class II PHA synthase; Polyhydroxyalkanoates; Saturation mutagenesis; Site directed mutagenesis; Substrate specificity

Indexed keywords

PHA SYNTHASES; POLYHYDROXYALKANOATES; SATURATION MUTAGENESIS; SITE DIRECTED MUTAGENESIS; SUBSTRATE SPECIFICITY;

AMINO ACIDS; BIOCHEMISTRY; COPOLYMERIZATION; ENZYME ACTIVITY; EXPERIMENTS; MUTAGENESIS;

SUBSTRATES;

3 HYDROXYBUTYRIC ACID; ACETYL COENZYME A ACETYLTRANSFERASE; ACETYL COENZYME A ACYLTRANSFERASE; ALANINE; AMINO ACID; MONOMER; POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYALKANOATE SYNTHASE CLASS II; UNCLASSIFIED DRUG; VALINE;

ARTICLE; BIOSYNTHESIS; ENZYME SPECIFICITY; GENE EXPRESSION; GENE LOCATION; GENE MUTATION; MOLECULAR MODEL; MUTAGENESIS; MUTATIONAL ANALYSIS; NONHUMAN; PSEUDOMONAS PUTIDA;

CLASS II PHA SYNTHASE; POLYHYDROXYALKANOATES; SATURATION MUTAGENESIS; SITE-DIRECTED MUTAGENESIS; SUBSTRATE SPECIFICITY;

ACYLTRANSFERASES; AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CATALYSIS; CLONING, MOLECULAR; CONSENSUS SEQUENCE; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION, MISSENSE; POLYHYDROXYALKANOATES; PROTEIN CONFORMATION; PSEUDOMONAS PUTIDA; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 84893460687     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2014.01.005     Document Type: Article

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