Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli

Applied Microbiology and Biotechnology

Volumn 90, Issue 2, 2011, Pages 603-614

  Yang, Taek Ho ^a   Jung, Yu Kyung ^b   Kang, Hye Ok ^a   Kim, Tae Wan ^a   Park, Si Jae ^d   Lee, Sang Yup ^b,c  

^a LG Chemical Ltd   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^c Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^d Korea Research Institute of Chemical Technology   (South Korea)

Author keywords

P(3HB co LA); PLA; Pseudomonas PhaC1 variants; Site directed mutagenesis

Indexed keywords

3-HYDROXYBUTYRATE; E. COLI; HOMOPOLYMERS; MOLE FRACTION; P(3HB-CO-LA); PHA SYNTHASES; PLA; POLY LACTIC ACID; POLYHYDROXYALKANOATES; POLYMER CONTENT; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS CHLORORAPHIS; PSEUDOMONAS PHAC1 VARIANTS; PSEUDOMONAS PUTIDA; PSEUDOMONAS SP; RECOMBINANT E. COLI; RECOMBINANT ESCHERICHIA COLI; SHORT CHAIN LENGTHS; SITE-DIRECTED MUTAGENESIS; SYNTHASES; TYPE II; WILD TYPES;

BIOCHEMISTRY; MOLECULAR WEIGHT; MUTAGENESIS; ORGANIC POLYMERS; POLYMERS; SUBSTRATES;

ESCHERICHIA COLI;

3 HYDROXYBUTYRYL COENZYME A; COENZYME A; COPOLYMER; LACTYL COENZYME A; POLYHYDROXYALKANOATE SYNTHASE; POLYLACTIC ACID; UNCLASSIFIED DRUG;

BACTERIUM; BIOENGINEERING; ENZYME ACTIVITY; ESTER; MUTATION; POLYMER; RECOMBINATION;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; BIOSYNTHESIS; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE EXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS CHLORORAPHIS; PSEUDOMONAS PUTIDA; PSEUDOMONAS RESINOVORANS; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

ACYL COENZYME A; ACYLTRANSFERASES; AMINO ACID SEQUENCE; COENZYME A-TRANSFERASES; DNA, RECOMBINANT; ESCHERICHIA COLI; LACTIC ACID; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; MUTATION; POLYMERS; PSEUDOMONAS; SEQUENCE ANALYSIS, DNA;

CLOSTRIDIUM PROPIONICUM; ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS CHLORORAPHIS; PSEUDOMONAS PUTIDA; PSEUDOMONAS RESINOVORANS; PSEUDOMONAS SP.; PSEUDOMONAS SP. 61-3;

EID: 79954952536     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-3077-2     Document Type: Article

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