Extracellular matrix domain formation as an indicator of chondrocyte dedifferentiation and hypertrophy

Tissue Engineering - Part C: Methods

Volumn 20, Issue 2, 2014, Pages 160-168

  Wu, Ling ^a   Gonzalez, Stephanie ^a   Shah, Saumya ^a   Kyupelyan, Levon ^a   Petrigliano, Frank A ^a   Mcallister, David R ^a   Adams, John S ^a,b   Karperien, Marcel ^c   Tuan, Tai Lan ^d   Benya, Paul D ^a   Evseenko, Denis ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF TWENTE   (Netherlands)

^d CHILDREN S HOSPITAL LOS ANGELES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARTILAGE; CELLS; COLLAGEN; CYTOLOGY; FLOW CYTOMETRY; HYDROGELS; POLYMERASE CHAIN REACTION; REPAIR;

ARTICULAR CHONDROCYTES; EXTRACELLULAR MATRICES; FLUORESCENCE ACTIVATED CELL SORTING; HYPERTROPHIC CHONDROCYTES; IMMUNOHISTOCHEMISTRY; QUANTITATIVE POLYMERASE CHAIN REACTION; THERMOREVERSIBLE HYDROGEL; THREE-DIMENSIONAL ENVIRONMENT;

BODY FLUIDS;

COLLAGEN TYPE 1; COLLAGEN TYPE 10; COLLAGEN TYPE 2; GLYCOSAMINOGLYCAN;

ADULT; ARTICLE; CARTILAGE CELL; CELL AGGREGATION; CELL DEDIFFERENTIATION; CELL DENSITY; CELL HYPERPLASIA; CELL ISOLATION; CELL SELECTION; EXTRACELLULAR MATRIX; EXTRACELLULAR MATRIX DOMAIN DETECTION; FETUS; FIBROCARTILAGE; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GROWTH PLATE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HYALINE CARTILAGE; HYDROGEL; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN;

ADULT; CELL DEDIFFERENTIATION; CELL SEPARATION; CELL SHAPE; CHONDROCYTES; COLLAGEN TYPE II; EXTRACELLULAR MATRIX; HUMANS; HYDROGELS; HYPERTROPHY; PHENOTYPE;

EID: 84893425793     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2013.0056     Document Type: Article

References (21)

1. Brittberg, M., Lindahl, A., Nilsson, A., Ohlsson, C., Isaksson, O., and Peterson, L. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 331, 889, 1994
2. Ryu, J.H., Kim, S.J., Kim, S.H., Oh, C.D., Hwang, S.G., Chun, C.H., et al. Regulation of the chondrocyte phenotype by betacatenin. Development 129, 5541, 2002
3. Yoon, Y.M., Kim, S.J., Oh, C.D., Ju, J.W., Song, W.K., Yoo, Y.J., et al. Maintenance of differentiated phenotype of articular chondrocytes by protein kinase C and extracellular signal-regulated protein kinase. J Biol Chem 277, 8412, 2002
4. Binette, F., McQuaid, D.P., Haudenschild, D.R., Yaeger, P.C., McPherson, J.M., and Tubo, R. Expression of a stable articular cartilage phenotype without evidence of hypertrophy by adult human articular chondrocytes in vitro. J Orthopaed Res 16, 207, 1998
5. Benya, P.D., and Shaffer, J.D. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30, 215, 1982
6. Bonaventure, J., Kadhom, N., Cohen-Solal, L., Ng, K.H., Bourguignon, J., Lasselin, C., et al. Reexpression of cartilagespecific genes by dedifferentiated human articular chondrocytes cultured in alginate beads. Exp Cell Res 212, 97, 1994
7. Pacifici, M., Golden, E.B., Adams, S.L., and Shapiro, I.M. Cell hypertrophy and type X collagen synthesis in cultured articular chondrocytes. Exp Cell Res 192, 266, 1991
8. Diaz-Romero, J., Gaillard, J.P., Grogan, S.P., Nesic, D., Trub, T., and Mainil-Varlet, P. Immunophenotypic analysis of human articular chondrocytes: Changes in surface markers associated with cell expansion in monolayer culture. J Cell Physiol 202, 731, 2005
9. Diaz-Romero, J., Nesic, D., Grogan, S.P., Heini, P., and Mainil-Varlet, P. Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells. J Cell Physiol 214, 75, 2008
10. Grogan, S.P., Barbero, A., Diaz-Romero, J., Cleton-Jansen, A.M., Soeder, S., Whiteside, R., et al. Identification of markers to characterize and sort human articular chondrocytes with enhanced in vitro chondrogenic capacity. Arthritis Rheum 56, 586, 2007
11. Wu, L., Leijten, J.C., Georgi, N., Post, J.N., van Blitterswijk, C.A., and Karperien, M. Trophic effects of mesenchymal stem cells increase chondrocyte proliferation and matrix formation. Tissue Eng Part A 17, 1425, 2011
12. Wu, L., Prins, H.J., Helder, M.N., van Blitterswijk, C.A., and Karperien, M. Trophic effects of mesenchymal stem cells in chondrocyte co-cultures are independent of culture conditions and cell sources. Tissue Eng Part A 18, 1542, 2012
13. Zuba-Surma, E.K., Kucia, M., Abdel-Latif, A., Lillard, J.W., Jr., and Ratajczak, M.Z. The ImageStream System: A key step to a new era in imaging. Folia Histochem Cytobiol 45, 279, 2007
14. Livak, K.J., and Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) Method. Methods 25, 402, 2001
15. De Maistre, E., Bene, M.C., Foliguet, B., Touati, F., and Faure, G.C. Centrifugation on Percoll gradient enhances fluorescent lectin binding on human sperm: A flow cytometric analysis. Arch Androl 37, 179, 1996
16. Ahmed, T.A., and Hincke, M.T. Strategies for articular cartilage lesion repair and functional restoration. Tissue Eng Part B Rev 16, 305, 2010
17. van Dongen, G.A., and Vosjan, M.J. Immuno-positron emission tomography: Shedding light on clinical antibody therapy. Cancer Biother Radiopharm 25, 375, 2010
18. Solorio, L.D., Vieregge, E.L., Dhami, C.D., and Alsberg, E. High-density cell systems incorporating polymer microspheres as microenvironmental regulators in engineered cartilage tissues. Tissue Eng Part B Rev 19, 209, 2013
19. Wu, L., Cai, X., Zhang, S., Karperien, M., and Lin, Y. Regeneration of articular cartilage by adipose tissue derived mesenchymal stem cells: Perspectives from stem cell biology and molecular medicine. J Cell Physiol 228, 938, 2013
20. Leijten, J.C., Georgi, N., Wu, L., van Blitterswijk, C.A., and Karperien, M. Cell sources for articular cartilage repair strategies: Shifting from monocultures to cocultures. Tissue Eng Part B Rev 19, 31, 2013
21. Brenner, J.M., Kunz, M., Tse, M.Y., Winterborn, A., Bardana, D.D., Pang, S.C., et al. Development of large engineered cartilage constructs from a small population of cells. Biotechnol Prog 29, 213, 2013