Characterization of calcium responses and electrical activity in differentiating mouse neural progenitor cells in vitro

Toxicological Sciences

Volumn 137, Issue 2, 2014, Pages 428-435

  de Groot, Martje W G D M ^a   Dingemans, Milou M L ^a   Rus, Katinka H ^a   de Groot, Aart ^a   Westerink, Remco H S ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Calcium homeostasis; Differentiating mouse neural progenitor cells; Electrical activity; Functional endpoints for in vitro neurotoxicity testing; Fura 2 single cell fluorescent microscopy; Multielectrode array

Indexed keywords

4 AMINOBUTYRIC ACID; ACETYLCHOLINE; ADENOSINE TRIPHOSPHATE; CALCIUM; DOPAMINE; FURA 2; GLUTAMIC ACID; NEUROTRANSMITTER RECEPTOR; SEROTONIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CALCIUM CELL LEVEL; CALCIUM SIGNALING; CELL TYPE; CELLULAR PARAMETERS; CONTROLLED STUDY; DEVELOPMENTAL NEUROTOXICITY TESTING; ELECTRIC ACTIVITY; GENE EXPRESSION; GLIA CELL; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; MEMBRANE DEPOLARIZATION; MOUSE; NERVE CELL DIFFERENTIATION; NERVE CELL NETWORK; NERVE FUNCTION; NERVE STIMULATION; NEURAL STEM CELL; NEUROTOXICITY; NEUROTRANSMITTER RELEASE; NONHUMAN; TOXICITY TESTING;

CALCIUM HOMEOSTASIS; DIFFERENTIATING MOUSE NEURAL PROGENITOR CELLS; ELECTRICAL ACTIVITY.; FUNCTIONAL ENDPOINTS FOR IN VITRO NEUROTOXICITY TESTING; FURA-2 SINGLE-CELL FLUORESCENT MICROSCOPY; MULTIELECTRODE ARRAY;

ANIMAL USE ALTERNATIVES; ANIMALS; CALCIUM; CALCIUM SIGNALING; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELLS, CULTURED; EMBRYONIC STEM CELLS; EVOKED POTENTIALS; IMMUNOHISTOCHEMISTRY; MICE; MICROELECTRODES; NEURONS; NEUROTRANSMITTER AGENTS;

EID: 84893361903     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kft261     Document Type: Article

References (16)

1. Araque, A., and Navarrete, M. (2010). Glial cells in neuronal network function. Philos. Trans. R. Soc. Lond. B Biol. Sci. 365, 2375-2381.
2. Azari, H., Sharififar, S., Rahman, M., Ansari, S., and Reynolds, B. A. (2011). Establishing embryonic mouse neural stem cell culture using the neurosphere assay. J. Vis. Exp. 47, pii: 2457, doi:10.3791/2457
3. Bard, J. L., Kaufman, M. H., Dubreuil, C., Brune, R. M., Burger, A., Baldock, R. A., and Davidson, D. R. (1998). An internet-accessible database of mouse developmental anatomy based on a systematic nomenclature. Mech. Dev. 74, 111-120.
4. Breier, J. M., Gassmann, K., Kayser, R., Stegeman, H., De Groot, D., Fritsche, E., and Shafer, T. J. (2010). Neural progenitor cells as models for highthroughput screens of developmental neurotoxicity: State of the science. Neurotoxicol. Teratol. 32, 4-15.
5. de Groot, M. W., Westerink, R. H., and Dingemans, M. M. (2013). Don't judge a neuron only by its cover: Neuronal function in in vitro developmental neurotoxicity testing. Toxicol. Sci. 132, 1-7.
6. Gassmann, K., Abel, J., Bothe, H., Haarmann-Stemmann, T., Merk, H. F., Quasthoff, K. N., Rockel, T. D., Schreiber, T., and Fritsche, E. (2010). Species-specific differential AhR expression protects human neural progenitor cells against developmental neurotoxicity of PAHs. Environ. Health Perspect. 118, 1571-1577.
7. Gassmann, K., Baumann, J., Giersiefer, S., Schuwald, J., Schreiber, T., Merk, H. F., and Fritsche, E. (2012). Automated neurosphere sorting and plating by the COPAS large particle sorter is a suitable method for high-throughput 3D in vitro applications. Toxicol. In Vitro 26, 993-1000.
8. Grynkiewicz, G., Poenie, M., and Tsien, R. Y. (1985). A new generation of Ca2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260, 3440-3450.
9. Hendriks, H. S., van Kleef, R. G., van den Berg, M., and Westerink, R. H. (2012). Multiple novel modes of action involved in the in vitro neurotoxic effects of tetrabromobisphenol-A. Toxicol. Sci. 128, 235-246.
10. Hogberg, H. T., Sobanski, T., Novellino, A., Whelan, M., Weiss, D. G., and Bal-Price, A. K. (2011). Application of micro-electrode arrays (MEAs) as an emerging technology for developmental neurotoxicity: Evaluation of domoic acid-induced effects in primary cultures of rat cortical neurons. Neurotoxicology 32, 158-168.
11. Lohmann, C. (2009). Calcium signaling and the development of specific neuronal connections. Prog. Brain Res. 175, 443-452.
12. McConnell, E. R., McClain, M. A., Ross, J., Lefew, W. R., and Shafer, T. J. (2012). Evaluation of multi-well microelectrode arrays for neurotoxicity screening using a chemical training set. Neurotoxicology 36, 34-41.
13. Moors, M., Rockel, T. D., Abel, J., Cline, J. E., Gassmann, K., Schreiber, T., Schuwald, J., Weinmann, N., and Fritsche, E. (2009). Human neurospheres as three-dimensional cellular systems for developmental neurotoxicity testing. Environ. Health Perspect. 117, 1131-1138.
14. Neher, E., and Sakaba, T. (2008). Multiple roles of calcium ions in the regulation of neurotransmitter release. Neuron 59, 861-872.
15. Novellino, A., Scelfo, B., Palosaari, T., Price, A., Sobanski, T., Shafer, T. J., Johnstone, A. F., Gross, G. W., Gramowski, A., Schroeder, O., et al. (2011). Development of micro-electrode array based tests for neurotoxicity: Assessment of interlaboratory reproducibility with neuroactive chemicals. Front. Neuroeng. 4, 4.
16. Robinette, B. L., Harrill, J. A., Mundy, W. R., and Shafer, T. J. (2011). In vitro assessment of developmental neurotoxicity: Use of microelectrode arrays to measure functional changes in neuronal network ontogeny. Front. Neuroeng. 4, 1.