Establishing embryonic mouse neural stem cell culture using the neurosphere assay

Journal of Visualized Experiments

Volumn , Issue 47, 2010, Pages

  Azari, Hassan ^a,b   Sharififar, Sharareh ^b   Rahman, Maryam ^b   Ansari, Saeed ^b   Reynolds, Brent A ^b  

^a SHIRAZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

Embryonic Neural Stem Cells; Expansion; Isolation; Issue 47; Neuroscience; Neurosphere Assay

Indexed keywords

EID: 79958830732     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2457     Document Type: Article

References (7)

1. Reynolds, B.A. & Weiss, S., Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255 (5052), 1707-1710 (1992).
2. Morshead, C.M. et al., Neural stem cells in the adult mammalian forebrain: a relatively quiescent subpopulation of subependymal cells. Neuron 13 (5), 1071-1082 (1994).
3. Craig, C.G. et al., In vivo growth factor expansion of endogenous subependymal neural precursor cell populations in the adult mouse brain. J Neurosci 16 (8), 2649-2658 (1996).
4. Golmohammadi, M.G. et al., Comparative analysis of the frequency and distribution of stem and progenitor cells in the adult mouse brain. Stem Cells 26 (4), 979-987 (2008).
5. Azari H, Rahman M, Sharififar S, Reynolds BA (2010). Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay. J Vis Exp. http://www.jove.com/index/details.stp?id=2393, doi: 10.3791/2393
6. Reynolds, B.A. & Rietze, R.L., Neural stem cells and neurospheres--re-evaluating the relationship. Nat Methods 2 (5), 333-336 (2005).
7. Louis, S.A. et al., Enumeration of neural stem and progenitor cells in the neural colony-forming cell assay. Stem Cells 26 (4), 988-996 (2008).