PiggyBac transposon plus insulators overcome epigenetic silencing to provide for stable signaling pathway reporter cell lines

PLoS ONE

Volumn 8, Issue 12, 2013, Pages

  Mossine, Valeri V ^a,b   Waters, James K ^b   Hannink, Mark ^a   Mawhinney, Thomas P ^a,b  

^a University of Missouri   (United States)

^b UNIVERSITY OF MISSOURI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYPOXIA INDUCIBLE FACTOR 1ALPHA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; TRANSCRIPTION FACTOR NRF2;

ANIMAL CELL; ARTICLE; CANCER CELL CULTURE; CELL STRAIN K 562; CELL STRAIN THP 1; CELL STRAIN TRAMP C2; CONTROLLED STUDY; DENDRITIC CELL; EPIGENETICS; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INSULATOR ELEMENT; INTRACELLULAR SIGNALING; INVERTED TERMINAL REPEAT; MACROPHAGE; MALE; MONOCYTE; MOUSE; NONHUMAN; PIGGYBAC TRANSPOSON; PROSTATE ADENOCARCINOMA; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REPORTER GENE; TRANSPOSON;

ANIMALS; CELL LINE, TUMOR; DNA TRANSPOSABLE ELEMENTS; GENE TRANSFER TECHNIQUES; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HEMATOPOIETIC STEM CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; LUCIFERASES; MICE;

EID: 84893205165     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0085494     Document Type: Article

References (42)

1. Naylor LH (1999) Reporter gene technology: the future looks bright. Biochem Pharmacol 58: 749-757. doi:10.1016/S0006-2952(99)00096-9. PubMed: 10449183.
2. Michelini E, Cevenini L, Mezzanotte L, Coppa A, Roda A (2010) Cell-based assays: fuelling drug discovery. Anal Bioanal Chem 398: 227-238. doi:10.1007/s00216-010-3933-z. PubMed: 20623273.
3. Maggi A, Ciana P (2005) Innovation: Reporter mice and drug discovery and development. Nat Rev Drug Discovery 4: 249-255. doi:10.1038/nrd1661. (Pubitemid 40372558)
4. Yagi K (2007) Applications of whole-cell bacterial sensors in biotechnology and environmental science. Appl Microbiol Biotechnol 73: 1251-1258. doi:10.1007/s00253-006-0718-6. PubMed: 17111136.
5. Emter R, Ellis G, Natsch A (2010) Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro. Toxicol Appl Pharmacol 245: 281-290. doi:10.1016/j.taap.2010.03.009. PubMed: 20307559.
6. Vandebriel RJ, Hoefnagel MHN (2012) Dendritic cell-based in vitro assays for vaccine immunogenicity. Hum Vaccines - Journal of Immunother 8: 1323-1325. doi:10.4161/hv.21350. PubMed: 22951585.
7. Kimber I, Dearman RJ, Basketter DA (2013) Dendritic cells and the assessment in vitro of skin sensitizing potential. Cutan Ocul Toxicol 32: 54-59. doi:10.3109/15569527.2012.692135. PubMed: 22668204.
8. Hansel TT, Johnston SL, Openshaw PJ (2013) Microbes and mucosal immune responses in asthma. Lancet 381: 861-873. doi:10.1016/S0140-6736(12)62202-8. PubMed: 23428115.
9. Kau AL, Ahern PP, Griffin NW, Goodman AL, Gordon JI (2011) Human nutrition, the gut microbiome and the immune system. Nature 474: 327-336. doi:10.1038/nature10213. PubMed: 21677749.
10. Conti L, Gessani S (2008) GM-CSF in the generation of dendritic cells from human blood monocyte precursors: recent advances. Immunobiology 213: 859-870. doi:10.1016/j.imbio.2008.07.017. PubMed: 18926300.
11. Lindemann C, Schilz AJ, Emons B, Baum C, Löw R et al. (2002) Down-regulation of retroviral transgene expression during differentiation of progenitor-derived dendritic cells. Exp Hematol 30: 150-157. doi: 10.1016/S0301-472X(01)00778-0. PubMed: 11823050.
12. Auwerx J (1991) The human leukemia cell line, THP-1: a multifacetted model for the study of monocyte-macrophage differentiation. Experientia 47: 22-31. doi:10.1007/BF02041244. PubMed: 1999239.
13. Qin Z (2012) The use of THP-1 cells as a model for mimicking the function and regulation of monocytes and macrophages in the vasculature. Atherosclerosis 221: 2-11. doi:10.1016/j.atherosclerosis.2011.09.003. PubMed: 21978918.
14. Ade N, Leon F, Pallardy M, Peiffer J-L, Kerdine-Romer S et al. (2009) HMOX1 and NQO1 genes are upregulated in response to contact sensitizers in dendritic cells and THP-1 cell line: role of the Keap1/Nrf2 pathway. Toxicol Sci 107: 451-460. PubMed: 19033392.
15. Burke B, Sumner S, Maitland N, Lewis CE (2002) Macrophages in gene therapy: cellular delivery vehicles and in vivo targets. J Leukoc Biol 72: 417-428. PubMed: 12223508.
16. Li Z, Michael IP, Zhou D, Nagy A, Rini JM (2013) Simple piggyBac transposon-based mammalian cell expression system for inducible protein production. Proc Natl Acad Sci U S A 110: 5004-5009. doi: 10.1073/pnas. 1218620110. PubMed: 23476064.
17. Maston GA, Evans SK, Green MR (2006) Transcriptional regulatory elements in the human genome. Annu Rev Genomics Hum Genet 7: 29-59. doi:10.1146/annurev. genom.7.080505.115623. PubMed: 16719718. (Pubitemid 44627921)
18. Recillas-Targa F (2006) Multiple strategies for gene transfer, expression, knockdown, and chromatin influence in mammalian cell lines and transgenic animals. Mol Biotechnol 34: 337-354. doi: 10.1385/MB:34:3:337. PubMed: 17284781.
19. Atkinson H, Chalmers R (2010) Delivering the goods: viral and non-viral gene therapy systems and the inherent limits on cargo DNA and internal sequences. Genetica 138: 485-498. doi:10.1007/s10709-009-9434-3. PubMed: 20084428.
20. Di Matteo M, Mátrai J, Belay E, Firdissa T, VandenDriessche T et al. (2012) PiggyBac toolbox. Methods Mol Biol 859: 241-254. doi: 10.1007/978-1-61779-603-6-14. PubMed: 22367876.
21. Li R, Zhuang Y, Han M, Xu T, Wu X (2013) piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice. Dis Models. Journal of Mech 6: 828-833.
22. Emery DW (2011) The use of chromatin insulators to improve the expression and safety of integrating gene transfer vectors. Hum Gene Ther 22: 761-774. doi:10.1089/hum.2010.233. PubMed: 21247248.
23. Ramezani A, Hawley RG (2010) Strategies to insulate lentiviral vector-expressed transgenes. Methods Mol Biol 614: 77-100. doi: 10.1007/978-1-60761-533-0-5. PubMed: 20225037.
24. Holm CK, Paludan SR, Fitzgerald KA (2013) DNA recognition in immunity and disease. Curr Opin Immunol 25: 13-18. doi:10.1016/j.coi.2012.12.006. PubMed: 23313533.
25. Schnoor M, Buers I, Sietmann A, Brodde MF, Hofnagel O et al. (2009) Efficient non-viral transfection of THP-1 cells. J Immunol Methods 344: 109-115. doi:10.1016/j.jim.2009.03.014. PubMed: 19345690.
26. Foster BA, Gingrich JR, Kwon ED, Madias C, Greenberg NM (1997) Characterization of prostatic epithelial cell lines derived from transgenic adenocarcinoma of the mouse prostate (TRAMP) model. Cancer Res 57: 3325-3330. PubMed: 9269988. (Pubitemid 27355467)
27. Jeet V, Ow K, Doherty E, Curley B, Russell PJ et al. (2008) Broadening of transgenic adenocarcinoma of the mouse prostate (TRAMP) model to represent late stage androgen depletion independent cancer. Prostate 68: 548-562. doi:10.1002/pros.20714. PubMed: 18247402. (Pubitemid 351500215)
28. McCabe MT, Low JA, Daignault S, Imperiale MJ, Wojno KJ et al. (2006) Inhibition of DNA methyltransferase activity prevents tumorigenesis in a mouse model of prostate cancer. Cancer Res 66: 385-392. doi:10.1158/0008-5472.CAN-05- 2020. PubMed: 16397253. (Pubitemid 43166046)
29. Camoriano M, Morey Kinney SR, Moser MT, Foster BA, Mohler JL et al. (2008) Phenotype-specific CpG island methylation events in a murine model of prostate cancer. Cancer Res 68: 4173-4182. doi: 10.1158/0008-5472.CAN-07-6715. PubMed: 18519676.
30. Migliaccio AR, Bengra C, Ling J, Pi W, Li C et al. (2000) Stable and unstable transgene integration sites in the human genome: extinction of the Green Fluorescent Protein transgene in K562 cells. Gene 256: 197-214. doi:10.1016/S0378-1119(00)00353-X. PubMed: 11054549.
31. Berges C, Naujokat C, Tinapp S, Wieczorek H, Höh A et al. (2005) A cell line model for the differentiation of human dendritic cells. Biochem Biophys Res Commun 333: 896-907. doi:10.1016/j.bbrc.2005.05.171. PubMed: 15963458. (Pubitemid 40854243)
32. Owen MR, Stamper IJ, Muthana M, Richardson GW, Dobson J et al. (2011) Mathematical modeling predicts synergistic antitumor effects of combining a macrophage-based, hypoxia-targeted gene therapy with chemotherapy. Cancer Res 71: 2826-2837. doi: 10.1158/1538-7445.AM2011-2826. PubMed: 21363914.
33. Corsini E, Aeby P, Python F, Nelissen I, Kjaer T et al. (2011) The use of myeloid cell lines for the identification of allergens. In: EL RoggenHU WeltzienH Hermans. Prog Novel Test Strategies In Vitro Assess Allergens. Trivandrum, India: Transworld Research Network. pp. 103-116.
34. Meir Y-JJ, Wu SC-Y (2011) Transposon-based vector systems for gene therapy clinical trials: challenges and considerations. Chang Gung Med J 34: 565-579. PubMed: 22196059.
35. Grabundzija I, Irgang M, Mátés L, Belay E, Matrai J et al. (2010) Comparative analysis of transposable element vector systems in human cells. Mol Ther 18: 1200-1209. doi:10.1038/mt.2010.47. PubMed: 20372108.
36. Li X, Burnight Erin R, Cooney Ashley L, Malani N, Brady T et al. (2013) piggyBac transposase tools for genome engineering. Proc Natl Acad Sci U S A 110: E2279-E2287. doi:10.1073/pnas.1305987110. PubMed: 23723351.
37. Voigt K, Gogol-Döring A, Miskey C, Chen W, Cathomen T et al. (2012) Retargeting Sleeping Beauty transposon insertions by engineered zinc finger DNA-binding domains. Mol Ther 20: 1852-1862. doi:10.1038/mt.2012.126. PubMed: 22776959.
38. Liang Q, Kong J, Stalker J, Bradley A (2009) Chromosomal mobilization and reintegration of Sleeping Beauty and PiggyBac transposons. Genesis 47: 404-408. doi:10.1002/dvg.20508. PubMed: 19391106.
39. Bradburne C, Robertson K, Thach D (2009) Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection. Genet Vaccines Ther 7: 6. doi:10.1186/1479-0556-7-6. PubMed: 19432988.
40. Baens M, Noels H, Broeckx V, Hagens S, Fevery S et al. (2006) The dark side of EGFP: defective polyubiquitination. PLOS ONE 1: e54. doi: 10.1371/journal.pone.0000054. PubMed: 17183684.
41. Leone C, Le Pavec G, Même W, Porcheray F, Samah B et al. (2006) Characterization of human monocyte-derived microglia-like cells. Glia 54: 183-192. doi:10.1002/glia.20372. PubMed: 16807899. (Pubitemid 44230855)
42. Takahashi N, Udagawa N, Kobayashi Y, Suda T (2007) Generation of osteoclasts in vitro, and assay of osteoclast activity. Methods Mol Med 135: 285-301. doi:10.1007/978-1-59745-401-8-18. PubMed: 17951666.