A cost-effective approach for detection of toxigenic Clostridium difficile: Toxigenic culture using ChromID Clostridium difficile agar

Journal of Clinical Microbiology

Volumn 52, Issue 2, 2014, Pages 671-673

  Luk, Shik ^a   To, Wing Kin ^a   Ng, Tak Keung ^a   Hui, Wai Ting ^a   Lee, Wing Keung ^a   Lau, Florence ^a   Ching, Almond Man Wai ^a  

^a PRINCESS MARGARET HOSPITAL   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR MEDIUM; ARTICLE; BACTERIUM COLONY; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM DIFFICILE INFECTION; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DIAGNOSTIC KIT; DIAGNOSTIC TEST ACCURACY STUDY; FECES ANALYSIS; INTERMETHOD COMPARISON; LIQUID FECES; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

AGAR; BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CHROMOGENIC COMPOUNDS; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; COST-BENEFIT ANALYSIS; CULTURE MEDIA; FECES; HUMANS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 84893126428     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.03113-13     Document Type: Article

References (20)

1. Bartlett JG, Moon N, Chang TW, Taylor N, Onderdonk AB. 1978. Role of Clostridium difficile in antibiotic-associated pseudomembranous colitis. Gastroenterology 75:778-782.
2. Planche T, Aghaizu A, Holliman R, Riley P, Poloniecki J, Breathnach A, Krishna S. 2008. Diagnosis of Clostridium difficile infection by toxin detection kits: a systematic review. Lancet Infect. Dis. 8:777-784. http://dx.doi.org/10. 1016/S1473-3099(08)70233-0.
3. Deshpande A, Pasupuleti V, Rolston DD, Jain A, Deshpande N, Pant C, Hernandez AV. 2011. Diagnostic accuracy of real-time polymerase chain reaction in detection of Clostridium difficile in the stool samples of patients with suspected Clostridium difficile infection: a metaanalysis. Clin. Infect. Dis. 53:e81-e90. http://dx.doi.org/10.1093/cid/cir505.
4. Carroll KC. 2011. Tests for the diagnosis of Clostridium difficile infection: the next generation. Anaerobe 17:170-174. http://dx.doi.org/10.1016/ j.anaerobe.2011.01.002.
5. Ferguson JK, Cheng AC, Gilbert GL, Gottlieb T, Korman T, McGregor A, Richards M, Roberts S, Robson J, Van Gessel H, Riley TV. 2011. Clostridium difficile laboratory testing in Australia and New Zealand: national survey results and Australasian Society for Infectious Diseases recommendations for best practice. Pathology 43:482-487. http://dx.doi.org/10.1097/PAT. 0b013e328348c9b4.
6. Peterson LR, Manson RU, Paule SM, Hacek DM, Robicsek A, Thomson RB, Jr, Kaul KL. 2007. Detection of toxigenic Clostridium difficile in stool samples by real-time polymerase chain reaction for the diagnosis of C. difficile-associated diarrhea. Clin. Infect. Dis. 45:1152-1160. http://dx.doi.org/10.1086/522185.
7. Marler LM, Siders JA, Wolters LC, Pettigrew Y, Skitt BL, Allen SD. 1992. Comparison of five cultural procedures for isolation of Clostridium difficile from stools. J. Clin. Microbiol. 30:514-516.
8. Cohen SH, Gerding DN, Johnson S, Kelly CP, Loo VG, McDonald LC, Pepin J, Wilcox MH, Society for Healthcare Epidemiology of America and Infectious Diseases Society of America. 2010. Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the Society for Healthcare Epidemiology of America (SHEA) and the Infectious Diseases Society of America (IDSA). Infect. Control. Hosp. Epidemiol. 31:431-455. http://dx.doi.org/10.1086/ 651706.
9. Reller ME, Lema CA, Perl TM, Cai M, Ross TL, Speck KA, Carroll KC. 2007. Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile. J. Clin. Microbiol. 45:3601-3605. http://dx.doi.org/10.1128/JCM.01305-07.
10. Fenner K, Widmer AF, Goy G, Rudin S, Frei R. 2008. Rapid and reliable diagnostic algorithm for detection of Clostridium difficile. J. Clin. Microbiol. 46:328-330. http://dx.doi.org/10.1128/JCM.01503-07.
11. Sharp SE, Ivie WM, Buckles MR, Coover DM, Pohl JC, Hatcher PA. 2009. A simple 3-step algorithm for improved laboratory detection of Clostridium difficile toxin without the need for tissue culture cytotoxicity neutralization assays. Diagn. Microbiol. Infect. Dis. 64:344-346. http://dx.doi.org/10.1016/j. diagmicrobio.2009.03.009.
12. Perry JD, Asir K, Halimi D, Orenga S, Dale J, Payne M, Carlton R, Evans J, Gould FK. 2010. Evaluation of a chromogenic culture medium for isolation of Clostridium difficile within 24 hours. J. Clin. Microbiol. 48:3852-3858. http://dx.doi.org/10.1128/JCM.01288-10.
13. Carson KC, Boseiwaqa LV, Thean SK, Foster NF, Riley TV. 2013. Isolation of Clostridium difficile from faecal specimens-a comparison of ChromID C. difficile agar and cycloserine cefoxitin fructose agar. J. Med. Microbiol. 62:1423-1427. http://dx.doi.org/10.1099/jmm.0.056515-0.
14. Walker AS, Eyre DW, Wyllie DH, Dingle KE, Harding RM, O'Connor L, Griffiths D, Vaughan A, Finney J, Wilcox MH, Crook DW, Peto TE. 2012. Characterisation of Clostridium difficile hospital ward-based transmission using extensive epidemiological data and molecular typing. PLoS Med. 9:e1001172. http://dx.doi.org/10.1371/journal.pmed.1001172.
15. Planche TD, Davies KA, Coen PG, Finney JM, Monahan IM, Morris KA, O'Connor L, Oakley SJ, Pope CF, Wren MW, Shetty NP, Crook DW, Wilcox MH. 2013. Differences in outcome according to Clostridium difficile testing method: a prospective multicentre diagnostic validation study of C difficile infection. Lancet Infect. Dis. 13:936-945. http://dx.doi.org/10.1016/S1473-3099(13)70200-7.
16. Dubberke ER, Han Z, Bobo L, Hink T, Lawrence B, Copper S, Hoppe-Bauer J, Burnham CD, Dunne WM. 2011. Impact of clinical symptoms on interpretation of diagnostic assays for Clostridium difficile infections. J. Clin. Microbiol. 49:2887-2893. http://dx.doi.org/10.1128/JCM.00891-11.
17. Sloan LM, Duresko BJ, Gustafson DR, Rosenblatt JE. 2008. Comparison of real-time PCR for detection of the tcdC gene with four toxin immunoassays and culture in diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 46:1996-2001. http://dx.doi.org/10.1128/JCM.00032-08.
18. Jayaratne PA, Monkman L, Broukhanski G, Pillai DR, Lee C. 2013. Real-time polymerase chain reaction method for detection of toxigenic Clostridium difficile from stools and presumptive identification of NAP1 clone. Diagn. Microbiol. Infect. Dis. 75:121-123. http://dx.doi.org/10.1016/j.diagmicrobio. 2012.10.002.
19. Bakker D, Smits WK, Kuijper E, Corver J. 2012. TcdC does not significantly repress toxin expression in Clostridium difficile 630-Erm. PLoS One 7:e43247. http://dx.doi.org/10.1371/journal.pone.0043247.
20. Knetsch CW, Hensgens MPM, Harmanus C, van der Bijl MW, Savelkoul PHM, Kuijper EJ, Corver J, van Leeuwen HC. 2011. Genetic markers for Clostridium difficile lineages linked to hypervirulence. Microbiology 157:3113-3123. http://dx.doi.org/10.1099/mic.0.051953-0.