Positively charged nanogold label allows the observation of fine cell filopodia and flagella in solution by atmospheric scanning electron microscopy

Microscopy Research and Technique

Volumn 77, Issue 2, 2014, Pages 153-160

  Nishiyama, Hidetoshi ^a   Teramoto, Kanae ^a   Suga, Mitsuo ^a   Sato, Chikara ^b  

^a JEOL LTD   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

ASEM; Filopodia; Flagella; Nanogold; Pili

Indexed keywords

ATMOSPHERIC PRESSURE; CELL MEMBRANES; CYTOLOGY; GOLD; SCANNING ELECTRON MICROSCOPY;

ASEM; FILOPODIUM; FLAGELLUM; HIGH RESOLUTION; NANO-GOLD; NANOGOLD PARTICLES; PILUS; POSITIVELY CHARGED; SAMPLE PRETREATMENT; TARGET STRUCTURE;

BACTERIA;

ANIMALIA; BACTERIA (MICROORGANISMS);

GOLD; NANOPARTICLE; PILI;

ANIMAL; ARTICLE; ASEM; BACTERIUM; CELL SURFACE; CHLOROCEBUS AETHIOPS; COS 1 CELL LINE; EPITHELIUM CELL; FILOPODIA; FLAGELLUM; METABOLISM; METHODOLOGY; NANOGOLD; SCANNING ELECTRON MICROSCOPY; STAINING; STATIC ELECTRICITY; ULTRASTRUCTURE;

ASEM; FILOPODIA; FLAGELLA; NANOGOLD; PILI;

ANIMALS; BACTERIA; CELL SURFACE EXTENSIONS; CERCOPITHECUS AETHIOPS; COS CELLS; EPITHELIAL CELLS; GOLD; MICROSCOPY, ELECTRON, SCANNING; NANOPARTICLES; STAINING AND LABELING; STATIC ELECTRICITY;

EID: 84892783068     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.22322     Document Type: Article

References (29)

1. Abrams IM, McBain JW. 1944. A closed cell for electron microscopy. J Appl Phys 15:607-609.
2. Brinton CC, Jr, Gemski P, Jr, Carnahan J. 1964. A new type of bacterial pilus genetically controlled by the fertility factor of E. Coli K 12 and its role in chromosome transfer. Proc Natl Acad Sci USA 52:776-783.
3. de Jonge N, Peckys DB, Kremers GJ, Piston DW. 2009. Electron microscopy of whole cells in liquid with nanometer resolution. Proc Natl Acad Sci USA 106:2159-2164.
4. Deerinck TJ, Martone M, Ellisman MH. 2006. Preparative methods for transmission electron microscopy. In: Spector DL, Goldman RD, editors. Basic methods in microscopy. New York: Spector Goldman. pp. 307-342.
5. Fukami A, Fukushima K, Kohyama N. 1990. Observation technique for wet clay minerals using film-sealed environmental cell equipment attached to high-resolution electron m icroscope. In: Bennett RH, Bryant WR, Hulbert MH, editors. Microstructure of fine-grained sediments: From mud to shale. Heidelberg: Springer. pp. 321-332.
6. Futamura Y, Kawatani M, Kazami S, Tanaka K, Muroi M, Shimizu T, Tomita K, Watanabe N, Osada H. 2012. Morphobase, an encyclopedic cell morphology database, and its use for drug target identification. Chem Biol 19:1620-1630.
7. Gai PL. 2002. Development of wet environment TEM (wet-ETEM) for in situ studies of liquid-catalyst reactions on the nanoscale. Microsc Microanal 8:21-28.
8. Gaietta GM, Giepmans BN, Deerinck TJ, Smith WB, Ngan L, Llopis J, Adams SR, Tsien RY, Ellisman MH. 2006. Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy. Proc Natl Acad Sci USA 103:17777-17782.
9. Hainfeld JF, Furuya FR, Powell RD, Liu W. 2001. DNA nanowires. In: Bailey GW, Price RL, Voelkl E, Musselman IH, editors. Proc. of 59th Annu. Mtng Micros. Soc. Am. Springer-Verlag, New York, NY. pp. 1034-1035.
10. Harris SL, Elliott DA, Blake MC, Must LM, Messenger M, Orndorff PE. 1990. Isolation and characterization of mutants with lesions affecting pellicle formation and erythrocyte agglutination by type 1 piliated Escherichia coli. J Bacteriol 172:6411-6418.
11. Inaga S, Katsumoto T, Tanaka K, Kameie T, Nakane H, Naguro T. 2007. Platium blue as an alternative to uranyl scetate for staining in transmission electron microscopy. Arch Histlol Cytol 70:43-49.
12. Jahn KA, Barton DA, Kobayashi K, Ratinac KR, Overall RL, Braet F. 2011. Correlative microscopy: Providing new understanding in the biomedical and plant sciences. Micron 43:565-582.
13. Kaehr B, Brinker CJ. 2010. Using bacterial cell growth to template catalytic asymmetry. Chem Commun 46:5268-5270.
14. Maruyama Y, Ebihara T, Nishiyama H, Konyuba Y, Senda M, Numaga-Tomita T, Senda T, Suga M, Sato C. 2012a. Direct observation of protein microcrystals in crystallization buffer by atmospheric scanning electron microscopy. Int J Mol Sci 13:10553-10567.
15. Maruyama Y, Ebihara T, Nishiyama H, Suga M, Sato C. 2012b. Immuno EM-OM correlative microscopy in solution by atmospheric scanning electron microscopy (ASEM). J Struct Biol 180:259-270.
16. Murai T, Maruyama Y, Mio K, Nishiyama H, Suga M, Sato C. 2010. Low cholesterol triggers membrane microdomain-dependent CD44 shedding and suppresses tumor cell migration. J Biol Chem 286:1999-2007.
17. Murphy TF, Kirkham C. 2002. Biofilm formation by nontypeable Haemophilus influenzae: Strain variability, outer membrane antigen expression and role of pili. BMC Microbiol 2:7.
18. Nishiyama H, Suga M, Ogura T, Maruyama Y, Koizumi M, Mio K, Kitamura S, Sato C. 2010. Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film. J Struct Biol 169:438-449.
19. Noh SM, Kim W-K, Kim SJ, Kim JM, Baek K-H, Oh Y-K. 2007. Enhanced cellular delivery and transfection efficiency of plasmid DNA using positively charged biocompatible colloidal gold nanoparticles. Biochim Biophys Acta 1770:747-752.
20. Pratt LA, Kolter R. 1998. Genetic analysis of Escherichia coli biofilm formation: Roles of flagella, motility, chemotaxis and type I pili. Mol Microbiol 30:285-293.
21. Prescianotto-Baschong C, Riezman H. 1998. Morphology of the yeast endocytic pathway. Mol Biol Cell 9:173-189.
22. Sartori A, Gatz R, Beck F, Rigort A, Baumeister W, Plitzko JM. 2007. Correlative microscopy: Bridging the gap between fluorescence light microscopy and cryo-electron tomography. J Struct Biol 160:135-145.
23. Sato C, Manaka S, Nakane D, Nishiyama H, Suga M, Nishizaka T, Miyata M, Maruyama Y. 2012. Rapid imaging of mycoplasma in solution using atmospheric scanning electron microscopy (ASEM). Biochem Biophys Res Commun 417:1213-1218.
24. Sato S, Takaki T, Nishiyama H, Omi T. 2013. TiO2 nanoparticles induced genotoxicity in cultured cells using atmospheric scanning electron microscopy (ASEM). J Cosmet Dermatol Sci Appl 3:121-123.
25. Suga M, Nishiyama H, Konyuba Y, Iwamatsu S, Watanabe Y, Yoshiura C, Ueda T, Sato C. 2011. The atmospheric scanning electron microscope with open sample space observes dynamic phenomena in liquid or gas. Ultramicroscopy 111:1650-1658.
26. Teramoto K. 2010. Rapid characterization of bacteria using ClairScope and SpiralTOF. JEOL News 45:8-41.
27. Thiberge S, Nechushtan A, Sprinzak D, Gileadi O, Behar V, Zik O, Chowers Y, Michaeli S, Schlessinger J, Moses E. 2004a. Scanning electron microscopy of cells and tissues under fully hydrated conditions. Proc Natl Acad Sci USA 101:3346-3351.
28. Thiberge S, Zik O, Moses E. 2004b. An apparatus for imaging liquids, cells, and other wet samples in the scanning electron microscopy. Rev Sci Instrum 75:2280-2289.
29. Turroni F, Serafini F, Foroni E, Duranti S, O'Connell Motherway M, Taverniti V, Mangifesta M, Milani C, Viappiani A, Roversi T, Sanchez B, Santoni A, Gioiosa L, Ferrarini A, Delledonne M, Margolles A, Piazza L, Palanza P, Bolchi A, Guglielmetti S, van Sinderen D, Ventura M. 2013. Role of sortase-dependent pili of Bifidobacterium bifidum PRL2010 in modulating bacterium-host interactions. Proc Natl Acad Sci USA 110:11151-11156.