Measurement of HMG CoA reductase activity in different human cell lines by ultra-performance liquid chromatography tandem mass spectrometry

Biochemical and Biophysical Research Communications

Volumn 443, Issue 2, 2014, Pages 641-645

  Kuzaj, Patricia ^a   Kuhn, Joachim ^a   Faust, Isabel ^a   Knabbe, Cornelius ^a   Hendig, Doris ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Cell culture; Cholesterol biosynthesis; Hydroxymethylglutaryl coenzyme A reductase; Mevalonate

Indexed keywords

CHOLESTEROL; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; LIPOPROTEIN; MEVALONOLACTONE;

ARTICLE; CALIBRATION; CHOLESTEROL SYNTHESIS; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; LIMIT OF QUANTITATION; MEASUREMENT; PRIORITY JOURNAL; SAMPLE; STANDARD; TANDEM MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

3-HYDROXY-3-METHYLGLUTARYL-COA; CELL CULTURE; CHOLESTEROL BIOSYNTHESIS; HMG-COA; HMGCR; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; LLOQ; LOWER LIMIT OF QUANTIFICATION; MEVALONATE; MEVALONIC ACID; MEVALONIC ACID LACTONE; MVA; MVL; ULTRA-PERFORMANCE LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY; UPLC-MS/MS;

ALGORITHMS; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME ACTIVATION; HEK293 CELLS; HEP G2 CELLS; HUMANS; HYDROXYMETHYLGLUTARYL COA REDUCTASES; SPECIMEN HANDLING; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 84892439425     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.12.013     Document Type: Article

References (24)

1. R. Zetterström The 1964 Nobel Prize for the discovery of the biosynthesis of cholesterol Acta Paediatr. 98 2009 1223 1227
2. F.D. Porter, and G.E. Herman Malformation syndromes caused by disorders of cholesterol synthesis J. Lipid Res. 52 2011 6 34
3. H.R. Waterham Defects of cholesterol biosynthesis FEBS Lett. 580 2006 5442 5449
4. L. Goedeke, and C. Fernández-Hernando Regulation of cholesterol homeostasis Cell. Mol. Life Sci. 2012 1 16
5. M.S. Brown, and J.L. Goldstein Multivalent feedback regulation of HMG CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth J. Lipid Res. 21 1980 505 517
6. J.L. Goldstein, and M.S. Brown et al. Regulation of the mevalonate pathway Nature 343 1990 425
7. J.W. Porter, and S.L. Spurgeon Biosynthesis of Isoprenoid Compounds 1981 Wiley New York
8. Z.H. Beg, J.A. Stonik, and H.B. Brewer 3-Hydroxy-3-methylglutaryl coenzyme A reductase: regulation of enzymatic activity by phosphorylation and dephosphorylation Proc. Natl. Acad. Sci. 75 1978 3678 3682
9. M.S. Brown, S.E. Dana, and J.L. Goldstein Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in human fibroblasts by lipoproteins Proc. Natl. Acad. Sci. 70 1973 2162 2166
10. K. Gibson, G. Hoffmann, A. Schwall, R. Broock, S. Aramaki, and L. Sweetman et al. 3-Hydroxy-3-methylglutaryl coenzyme A reductase activity in cultured fibroblasts from patients with mevalonate kinase deficiency: differential response to lipid supplied by fetal bovine serum in tissue culture medium J. Lipid Res. 31 1990 515 521
11. G. Popjak, G. Boehm, T.S. Parker, J. Edmond, P.A. Edwards, and A.M. Fogelman Determination of mevalonate in blood plasma in man and rat. Mevalonate "tolerance" tests in man J. Lipid Res. 20 1979 716 728
12. D.J. Shapiro, J.L. Nordstrom, J.J. Mitschelen, V.W. Rodwell, and R.T. Schimke Micro assay for 3-hdyroxy-3-methylglutaryl-CoA reductase in rat liver and in L-cell fibroblasts Biochim. Biophys. Acta (BBA)-Enzymol. 370 1974 369 377
13. M. Hiramatsu, A. Hayashi, H. Hidaka, H. Ueshima, and T. Kanno Enzyme immunoassay of urinary mevalonic acid and its clinical application Clin. Chem. 44 1998 2152 2157
14. T. Yoshida, A. Honda, N. Tanaka, Y. Matsuzaki, B. He, and T. Osuga et al. Simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma by gas chromatography - mass spectrometry as indices of cholesterol and bile acid biosynthesis J. Chromatogr. B Biomed. Sci. Appl. 613 1993 185 193
15. G. Nicolau, S. Shefer, G. Salen, and E. Mosbach Determination of hepatic 3-hydroxy-3-methylglutaryl CoA reductase activity in man J. Lipid Res. 15 1974 94 98
16. J. Waldron, and C. Webster Liquid chromatography-tandem mass spectrometry method for the measurement of serum mevalonic acid: a novel marker of hydroxymethylglutaryl coenzyme A reductase inhibition by statins Ann. Clin. Biochem. 48 2011 223 232
17. A. Honda, Y. Mizokami, Y. Matsuzaki, T. Ikegami, M. Doy, and H. Miyazaki Highly sensitive assay of HMG-CoA reductase activity by LC-ESI-MS/MS J. Lipid Res. 48 2007 1212 1220
18. E. Kindt, G. Szekely-Klepser, and S.T. Fountain The validation of a simple LC/MS/MS method for determining the level of mevalonic acid in human plasma Biomed. Chromatogr. 25 2011 323 329
19. J. Kuhn, C. Götting, and K. Kleesiek et al. Simultaneous measurement of amiodarone and desethylamiodarone in human plasma and serum by stable isotope dilution liquid chromatography-tandem mass spectrometry assay J. Pharm. Biomed. Anal. 51 2010 210
20. P.M. Bossuyt, J.B. Reitsma, D.E. Bruns, C.A. Gatsonis, P.P. Glasziou, and L.M. Irwig et al. The STARD statement for reporting studies of diagnostic accuracy: explanation and elaboration Ann. Intern. Med. 138 2003 W1 12
21. T. Yoshida, A. Honda, H. Miyazaki, and Y. Matsuzaki Determination of key intermediates in cholesterol and bile acid biosynthesis by stable isotope dilution mass spectrometry Anal. Chem. Insights 3 2008 45
22. G. Saini, T. Wani, A. Gautam, B. Varshney, T. Ahmed, and K. Rajan et al. Validation of the LC-MS/MS method for the quantification of mevalonic acid in human plasma and determination of the matrix effect J. Lipid Res. 47 2006 2340 2345
23. M.Y. Ndong-Akoume, D. Mignault, S. Perwaiz, G.L. Plaa, and I.M. Yousef Simultaneous evaluation of HMG-CoA reductase and cholesterol 7α-hydroxylase activities by electrospray tandem MS Lipids 37 2002 1101 1107
24. S.M. Houten, M.S. Schneiders, R.J.A. Wanders, and H.R. Waterham Regulation of isoprenoid/cholesterol biosynthesis in cells from mevalonate kinase-deficient patients J. Biol. Chem. 278 2003 5736 5743