Quantitative real-time PCR combined with propidium monoazide for the selective quantification of viable periodontal pathogens in an in vitro subgingival biofilm model

Journal of Periodontal Research

Volumn 49, Issue 1, 2014, Pages 20-28

  Sanchez M C ^a   Marin M J ^a   Figuero, E ^a   Llama Palacios, A ^a   Leon R ^b   Blanc, V ^b   Herrera, D ^a   Sanz, M ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b Parc Tecnologic del Valles   (Spain)

Author keywords

Aggregatibacter actinomycetemcomitans; Antimicrobial agents; Fusobacterium nucleatum; Oral biofilm; Porphyromonas gingivalis; Propidium monoazide; Quantitative real time PCR

Indexed keywords

2 PROPANOL; ANTIINFECTIVE AGENT; AZIDE; BACTERIAL DNA; COLORING AGENT; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE;

ACTINOMYCES; AGGREGATIBACTER ACTINOMYCETEMCOMITANS; ANALOGS AND DERIVATIVES; BACTERIAL LOAD; BIOFILM; CHEMISTRY; CLASSIFICATION; DIAGNOSTIC USE; DRUG EFFECTS; FUSOBACTERIUM NUCLEATUM; HUMAN; ISOLATION AND PURIFICATION; MICROBIAL VIABILITY; PORPHYROMONAS GINGIVALIS; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; SALIVA; STREPTOCOCCUS ORALIS; TIME; VEILLONELLA;

2-PROPANOL; ACTINOMYCES; AGGREGATIBACTER ACTINOMYCETEMCOMITANS; ANTI-INFECTIVE AGENTS; AZIDES; BACTERIAL LOAD; BIOFILMS; COLORING AGENTS; DNA, BACTERIAL; FUSOBACTERIUM NUCLEATUM; HUMANS; MICROBIAL VIABILITY; PORPHYROMONAS GINGIVALIS; PROPIDIUM; REAL-TIME POLYMERASE CHAIN REACTION; SALIVA; STREPTOCOCCUS ORALIS; TIME FACTORS; VEILLONELLA;

EID: 84891824737     PISSN: 00223484     EISSN: 16000765     Source Type: Journal    
DOI: 10.1111/jre.12073     Document Type: Article

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