Enumeration of viable Enterococcus faecalis, a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real-time polymerase chain reaction

Microbiology and Immunology

Volumn 55, Issue 12, 2011, Pages 889-892

  Takahashi, Yusuke ^a   Yoshida, Akihiro ^a   Nagayoshi, Masato ^a   Kitamura, Chiaki ^a   Nishihara, Tatsuji ^a   Awano, Shuji ^a   Ansai, Toshihiro ^a  

^a KYUSHU DENTAL COLLEGE   (Japan)

Author keywords

Apical periodontitis; Enterococcus faecalis; Propidium monoazide; Viable cell

Indexed keywords

PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BACTERIAL VIABILITY; BACTERIUM DETECTION; BACTERIUM ISOLATION; CONTROLLED STUDY; ENTEROCOCCUS FAECALIS; HUMAN; HUMAN TISSUE; NONHUMAN; PROCESS DEVELOPMENT; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; TOOTH PERIAPICAL DISEASE;

AZIDES; COLONY COUNT, MICROBIAL; DENTAL PULP CAVITY; ENTEROCOCCUS FAECALIS; GENES, BACTERIAL; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; MICROBIAL VIABILITY; PERIAPICAL PERIODONTITIS; PROPIDIUM; REAL-TIME POLYMERASE CHAIN REACTION;

ENTEROCOCCUS FAECALIS;

EID: 84855252026     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2011.00390.x     Document Type: Article

References (12)

1. Bonten M.J., Willems R., Weinstein R.A. (2001) Vancomycin-resistant enterococci: why are they here, and where do they come from? Lancet Infect Dis 1: 314-25.
2. Sun J., Song X., Kristiansen B.E., Kjaereng A., Willems R.J., Eriksen H.M., Sundsfjord A., Sollid J.E. (2009) Occurrence, population structure, and antimicrobial resistance of enterococci in marginal and apical periodontitis. J Clin Microbiol 47: 2218-25.
3. Peciuliene V., Balciuniene I., Eriksen H.M., Haapasalo M. (2000) Isolation of Enterococcus faecalis in previously root-filled canals in a Lithuanian population. J Endod 26: 593-5.
4. Yoshida A., Suzuki N., Nakano Y., Kawada M., Oho T., Koga T. (2003) Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus. J Clin Microbiol 41: 4438-41.
5. Nagashima S., Yoshida A., Suzuki N., Ansai T., Takehara T. (2005) Use of the genomic subtractive hybridization technique to develop a real-time PCR assay for quantitative detection of Prevotella spp. in oral biofilm samples. J Clin Microbiol 43: 2948-51.
6. Nocker A., Cheung C.Y., Camper A.K. (2006) Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J Microbiol Methods 67: 310-20.
7. Nocker A., Camper A.K. (2006) Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Appl Environ Microbiol 72: 1997-2004.
8. Pan Y., Breidt F. Jr. (2007) Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells. Appl Environ Microbiol 73: 8028-31.
9. Hixon S.C., White W.E. Jr, Yielding K.L. (1975) Selective covalent binding of an ethidium analog to mitochondrial DNA with production of petite mutants in yeast by photoaffinity labelling. J Mol Biol 92: 319-29.
10. Lee J.L., Levin R.E. (2006) Use of ethidium bromide monoazide for quantification of viable and dead mixed bacterial flora from fish fillets by polymerase chain reaction. J Microbiol Methods 67: 456-62.
11. Chen H., Wang R.F., Cerniglia C.E. (2004) Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis. Protein Expr Purif 34: 302-10.
12. Kato H., Yoshida A., Ansai T., Watari H., Notomi T., Takehara T. (2007) Loop-mediated isothermal amplification method for the rapid detection of Enterococcus faecalis in infected root canals. Oral Microbiol Immunol 22: 131-5.